{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T15:18:53Z","timestamp":1780499933715,"version":"3.54.1"},"reference-count":48,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T00:00:00Z","timestamp":1576195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA 2146\/22-1"],"award-info":[{"award-number":["HA 2146\/22-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003542","name":"Ministerium f\u00fcr Wissenschaft, Forschung und Kunst Baden-W\u00fcrttemberg","doi-asserted-by":"publisher","award":["7132000401"],"award-info":[{"award-number":["7132000401"]}],"id":[{"id":"10.13039\/501100003542","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universit\u00e4t T\u00fcbingen","award":["5132000501"],"award-info":[{"award-number":["5132000501"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>The transition to reproduction is a crucial step in the life cycle of any organism. In Arabidopsis thaliana the establishment of reproductive growth can be divided into two phases: Firstly, cauline leaves with axillary meristems are formed and internode elongation begins. Secondly, lateral meristems develop into flowers with defined organs. Floral shoots are usually determinate and suppress the development of lateral shoots. Here, we describe a transposon insertion mutant in the Nossen accession with defects in floral development and growth. Most strikingly is the outgrowth of stems from the axillary bracts of the primary flower carrying secondary flowers. Therefore, we named this mutant flower-in-flower (fif). However, the transposon insertion in the annotated gene is not the cause for the fif phenotype. By means of classical and genome sequencing-based mapping, the mutation responsible for the fif phenotype was found to be in the LEAFY gene. The mutation, a G-to-A exchange in the second exon of LEAFY, creates a novel lfy allele and results in a cysteine-to-tyrosine exchange in the \u03b11-helix of LEAFY\u2019s DNA-binding domain. This exchange abolishes target DNA-binding, whereas subcellular localization and homomerization are not affected. To explain the strong fif phenotype against these molecular findings, several hypotheses are discussed.<\/jats:p>","DOI":"10.3390\/plants8120599","type":"journal-article","created":{"date-parts":[[2019,12,13]],"date-time":"2019-12-13T11:27:22Z","timestamp":1576236442000},"page":"599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Striking Flower-in-Flower Phenotype of Arabidopsis thaliana Nossen (No-0) is Caused by a Novel LEAFY Allele"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8049-9934","authenticated-orcid":false,"given":"Anne","family":"Mohrholz","sequence":"first","affiliation":[{"name":"Center for Plant Molecular Biology, Eberhard Karls University T\u00fcbingen, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2046-2109","authenticated-orcid":false,"given":"Hequan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Gl\u00f6ckner","sequence":"additional","affiliation":[{"name":"Center for Plant Molecular Biology, Eberhard Karls University T\u00fcbingen, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Hummel","sequence":"additional","affiliation":[{"name":"Center for Plant Molecular Biology, Eberhard Karls University T\u00fcbingen, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8656-5963","authenticated-orcid":false,"given":"\u00dcner","family":"Kolukisaoglu","sequence":"additional","affiliation":[{"name":"Center for Plant Molecular Biology, Eberhard Karls University T\u00fcbingen, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korbinian","family":"Schneeberger","sequence":"additional","affiliation":[{"name":"Department of Plant Developmental Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2150-6970","authenticated-orcid":false,"given":"Klaus","family":"Harter","sequence":"additional","affiliation":[{"name":"Center for Plant Molecular Biology, Eberhard Karls University T\u00fcbingen, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1104\/pp.117.1.1","article-title":"The control of flowering time and floral identity in Arabidopsis","volume":"117","author":"Pineiro","year":"1998","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/S0168-9525(97)01309-7","article-title":"To be, or not to be, a flower\u2013control of floral meristem identity","volume":"14","author":"Ma","year":"1998","journal-title":"Trends Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/S1360-1385(98)01369-7","article-title":"The making of a flower: Control of floral meristem identity in Arabidopsis","volume":"4","author":"Pidkowich","year":"1999","journal-title":"Trends Plant Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.pbi.2016.09.003","article-title":"A flower is born: An update on Arabidopsis floral meristem formation","volume":"35","author":"Denay","year":"2017","journal-title":"Curr. 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