{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:53:27Z","timestamp":1790996007673,"version":"4.1.0"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordinaci\u00f3n de la Investigaci\u00f3n Cient\u00edfica (UMSNH)","award":["2.22"],"award-info":[{"award-number":["2.22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Plants face a variety of biotic and abiotic stresses including attack by microbial phytopathogens and nutrient deficiencies. Some bacterial volatile organic compounds (VOCs) activate defense and iron-deficiency responses in plants. To establish a relationship between defense and iron deficiency through VOCs, we identified key genes in the defense and iron-deprivation responses of the legume model Medicago truncatula and evaluated the effect of the rhizobacterial VOC N,N-dimethylhexadecylamine (DMHDA) on the gene expression in these pathways by RT-qPCR. DMHDA increased M. truncatula growth 1.5-fold under both iron-sufficient and iron-deficient conditions compared with untreated plants, whereas salicylic acid and jasmonic acid decreased growth. Iron-deficiency induced iron uptake and defense gene expression. Moreover, the effect was greater in combination with DMHDA. Salicylic acid, Pseudomonas syringae, jasmonic acid, and Botrytis cinerea had inhibitory effects on growth and iron response gene expression but activated defense genes. Taken together, our results showed that the VOC DMHDA activates defense and iron-deprivation pathways while inducing a growth promoting effect unlike conventional phytohormones, highlighting that DMHDA does not mimic jasmonic acid but induces an alternative pathway. This is a novel aspect in the complex interactions between biotic and abiotic stresses.<\/jats:p>","DOI":"10.3390\/plants9050624","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T10:27:19Z","timestamp":1589452039000},"page":"624","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Bacterial Compound N,N-Dimethylhexadecylamine Modulates Expression of Iron Deficiency and Defense Response Genes in Medicago truncatula Independently of the Jasmonic Acid Pathway"],"prefix":"10.3390","volume":"9","author":[{"given":"Vicente","family":"Montejano-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Edifico B3, Ciudad Universitaria, Morelia 58030, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Garc\u00eda-Pineda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Edifico B3, Ciudad Universitaria, Morelia 58030, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9354-7176","authenticated-orcid":false,"given":"Eduardo","family":"Valencia-Cantero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones Qu\u00edmico Biol\u00f3gicas, Universidad Michoacana de San Nicol\u00e1s de Hidalgo, Edifico B3, Ciudad Universitaria, Morelia 58030, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1146\/annurev-phyto-082712-102340","article-title":"Induced systemic resistance by beneficial microbes","volume":"52","author":"Pieterse","year":"2014","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1146\/annurev.arplant.57.032905.105346","article-title":"A renaissance of elicitors: Perception of microbe-associated molecular patterns and danger signals by pattern-recognition receptors","volume":"60","author":"Boller","year":"2009","journal-title":"Annu. Rev. Plant. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.pbi.2015.05.029","article-title":"Cues from chewing insects the intersection of DAMPs, HAMPs, MAMPs and effectors","volume":"26","author":"Acevedo","year":"2015","journal-title":"Curr. Opin. Plant Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1186\/s12870-016-0921-2","article-title":"DAMPs, MAMPs, and NAMPs in plant innate immunity","volume":"16","author":"Choi","year":"2016","journal-title":"BMC Plant Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1038\/nature05286","article-title":"The plant immune system","volume":"444","author":"Jones","year":"2006","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3389\/fpls.2013.00030","article-title":"Long-distance communication and signal amplification in systemic acquired resistance","volume":"4","author":"Shah","year":"2013","journal-title":"Front. Plant Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/nchembio.164","article-title":"Networking by small-molecule hormones in plant immunity","volume":"5","author":"Pieterse","year":"2009","journal-title":"Nat. Chem. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0065-2296(09)51006-3","article-title":"Rhizobacteria-induced systemic resistance","volume":"51","author":"Ofte","year":"2009","journal-title":"Adv. Bot. Res."},{"key":"ref_9","first-page":"135","article-title":"Significance of inducible defense-related proteins in infected plants","volume":"44","author":"Rep","year":"2010","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.chom.2019.07.006","article-title":"Stressed out about hormones: How plants orchestrate immunity","volume":"26","author":"Chory","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1146\/annurev.phyto.43.040204.135923","article-title":"Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens","volume":"43","author":"Glazebrook","year":"2005","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/ejss.12158","article-title":"Rhizospheric organic compounds in the soil-microorganism-plant system: Their role in iron availability","volume":"65","author":"Mimmo","year":"2014","journal-title":"Eur. J. Soil Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1104\/pp.104.3.815","article-title":"Iron: Nutritious, noxious, and not readily available","volume":"104","author":"Guerinot","year":"1994","journal-title":"Plant Physiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.plaphy.2011.01.026","article-title":"Towards a knowledge-based correction of iron chlorosis","volume":"49","year":"2011","journal-title":"Plant Physiol. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1111\/j.1469-8137.2009.02908.x","article-title":"Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots","volume":"183","author":"Santi","year":"2009","journal-title":"New Phytol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/17800","article-title":"A ferric-chelate reductase for iron uptake from soils","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5624","DOI":"10.1073\/pnas.93.11.5624","article-title":"A novel ironregulated metal transporter from plants identified by functional expression in yeast","volume":"93","author":"Eide","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3400","DOI":"10.1105\/tpc.104.024315","article-title":"The essential bHLH protein FIT1 is required for the iron deficiency response","volume":"16","author":"Colangelo","year":"2004","journal-title":"Plant Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1038\/cr.2008.26","article-title":"FIT interacts with AtbHLH38 and AtbHLH39 in regulating iron uptake gene expression for iron homeostasis in Arabidopsis","volume":"18","author":"Yuan","year":"2008","journal-title":"Cell Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1093\/mp\/sss089","article-title":"Requirement and functional redundancy of Ib subgroup bHLH proteins for iron deficiency responses and uptake in Arabidopsis thaliana","volume":"6","author":"Wang","year":"2013","journal-title":"Mol. Plant."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1104\/pp.113.220426","article-title":"Mutually exclusive alterations in secondary metabolism are critical for the uptake of insoluble iron compounds by Arabidopsis and Medicago truncatula","volume":"162","author":"Lin","year":"2013","journal-title":"Plant Physiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.resmic.2015.04.004","article-title":"Effects of plant antimicrobial phenolic compounds on virulence of the genus Pectobacterium","volume":"166","author":"Joshi","year":"2015","journal-title":"Res. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1094\/MPMI-05-14-0142-R","article-title":"\u03b2-Aminobutyric acid (BABA)-induced resistance in Arabidopsis thaliana: Link with iron homeostasis","volume":"27","author":"Koen","year":"2014","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1111\/j.1365-313X.2008.03775.x","article-title":"NRAMP genes function in Arabidopsis thaliana resistance to Erwinia chrysanthemi infection","volume":"58","author":"Segond","year":"2009","journal-title":"Plant J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.plaphy.2016.04.025","article-title":"Paenibacillus polymyxa BFKC01 enhances plant iron absorption via improved root systems and activated iron acquisition mechanisms","volume":"105","author":"Zhou","year":"2016","journal-title":"Plant Physiol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/s11104-010-0583-z","article-title":"A volatile organic compound analysis from Arthrobacter agilis identifies dimethylhexadecylamine, an amino-containing lipid modulating bacterial growth and Medicago sativa morphogenesis in vitro","volume":"339","year":"2011","journal-title":"Plant Soil"},{"key":"ref_27","first-page":"1","article-title":"Rhizobacterium Arthrobacter agilis UMCV2 increases organ-specific expression of FRO genes in conjunction with genes associated with the systemic resistance pathways of Medicago truncatula","volume":"40","year":"2018","journal-title":"Acta Physiol. Plant."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2691","DOI":"10.1111\/pce.13016","article-title":"Airborne signals from Trichoderma fungi stimulate iron uptake responses in roots resulting in priming of jasmonic acid-dependent defences in shoots of Arabidopsis thaliana and Solanum lycopersicum","volume":"40","author":"VanWees","year":"2017","journal-title":"Plant Cell Environ."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1007\/s12223-013-0243-9","article-title":"Medicago truncatula increases its iron-uptake mechanisms in response to volatile organic compounds produced by Sinorhizobium meliloti","volume":"58","author":"Santoyo","year":"2013","journal-title":"Folia Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.biocontrol.2014.11.011","article-title":"Characterization of the antifungal and plant-growth promoting effects of diffusible and volatile organic compoundd produced by Pseudomonas fluorescens strains","volume":"81","author":"Santoyo","year":"2015","journal-title":"Biol. Control"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11104-012-1263-y","article-title":"Arthrobacter agilis UMCV2 induces iron acquisition in Medicago truncatula (strategy I plant) in vitro via dimethylhexadecylamine emission","volume":"362","author":"Santoyo","year":"2013","journal-title":"Plant Soil"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1007\/s00344-015-9495-8","article-title":"Volatile organic compounds produced by the rhizobacterium Arthrobacter agilis UMCV2 modulate Sorghum bicolor (strategy II plant) morphogenesis and SbFRO1 transcription in vitro","volume":"34","author":"Santoyo","year":"2015","journal-title":"J. Plant Growth Regul."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1007\/s00709-016-1031-6","article-title":"N,N-dimethyl hexadecylamine and related amines regulate root morphogenesis via jasmonic acid signaling in Arabidopsis thaliana","volume":"254","year":"2017","journal-title":"Protoplasma"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s11103-017-0694-5","article-title":"Volatile compounds from beneficial or pathogenic bacteria differentially regulate root exudation, transcription of iron transporters, and defense signaling pathways in Sorghum bicolor","volume":"96","author":"Santoyo","year":"2018","journal-title":"Plant Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1105\/tpc.114.132704","article-title":"The bHLH transcription factor bHLH104 interacts with IAA-LEUCINE RESISTANT3 and modulates iron homeostasis in Arabidopsis","volume":"27","author":"Zhang","year":"2015","journal-title":"Plant Cell"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.4238\/2012.October.9.7","article-title":"Identification and expression analysis of multiple FRO gene copies in Medicago truncatula","volume":"11","author":"Santoyo","year":"2012","journal-title":"Genet. Mol. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1007\/s11103-005-5567-7","article-title":"Defensin gene family in Medicago truncatula: Structure, expression and induction by signal molecules","volume":"58","author":"Hanks","year":"2005","journal-title":"Plant Mol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1016\/j.plaphy.2009.07.009","article-title":"Time course induction of several key enzymes in Medicago truncatula roots in response to Fe deficiency","volume":"47","author":"Andaluz","year":"2009","journal-title":"Plant Physiol. Biochem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1111\/j.1364-3703.2012.00790.x","article-title":"Iron deficiency affects plant defence responses and confers resistance to Dickeya dadantii and Botrytis cinereal","volume":"13","author":"Kieu","year":"2012","journal-title":"Mol. Plant Pathol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9175","DOI":"10.1038\/s41598-017-08497-7","article-title":"The Arabidopsis defensin gene, AtPDF1.1, mediates defence against Pectobacterium carotovorum subsp. carotovorum via an iron-withholding defence system","volume":"7","author":"Hsiao","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1111\/tpj.12440","article-title":"The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3\/EIL1 through subunit MED25","volume":"77","author":"Yang","year":"2014","journal-title":"Plant J."},{"key":"ref_42","first-page":"1333","article-title":"Role and functioning of bHLH transcription factors in jasmonate signaling","volume":"68","author":"Goossens","year":"2017","journal-title":"J. Exp. Bot."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4294","DOI":"10.1105\/tpc.112.103317","article-title":"The Arabidopsis mediator complex subunit 16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate\/ethylene-induced defense pathways","volume":"24","author":"Zhang","year":"2012","journal-title":"Plant Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.tplants.2013.01.006","article-title":"Signal from chloroplasts and mitochondria for iron homeostasis regulation","volume":"18","author":"Vigani","year":"2013","journal-title":"Trends Plant Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0176-1617(96)80173-8","article-title":"Effects of salicylic acid on growth and photosynthesis in barley plants","volume":"149","author":"Pancheva","year":"1996","journal-title":"J. Plant Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-10634-1","article-title":"Antagonistic interaction between jasmonic acid and cytokinin in xylem development","volume":"7","author":"Jang","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3389\/fpls.2019.00006","article-title":"The transcriptional control of iron homeostasis in plants: A tale of bHLH transcription factors?","volume":"10","author":"Gao","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1111\/j.1365-313X.2006.02712.x","article-title":"WRKY70 modulates the selection of signaling pathways in plant defense","volume":"46","author":"Li","year":"2006","journal-title":"Plant J."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1104\/pp.15.01827","article-title":"Two bHLH transcription factors, bHLH34 and bHLH104, regulate iron homeostasis in Arabidopsis thaliana","volume":"170","author":"Li","year":"2016","journal-title":"Plant Physiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1007\/s11103-016-0486-3","article-title":"Jasmonate signaling is activated in the very early stages of iron deficiency responses in rice roots","volume":"4","author":"Kobayashi","year":"2016","journal-title":"Plant Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4179","DOI":"10.1093\/jxb\/erw196","article-title":"Involvement of endogenous salicylic acid in iron-deficiency responses in Arabidopsis","volume":"67","author":"Shen","year":"2016","journal-title":"J. Exp. Bot."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1104\/pp.107.113829","article-title":"MYB72 is required in early signaling steps of rhizobacteria-induced systemic resistance in Arabidopsis","volume":"146","author":"Verhagen","year":"2008","journal-title":"Plant Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3389\/fpls.2019.00287","article-title":"Induced systemic resistance (ISR) and Fe deficiency responses in dicot plants","volume":"10","author":"Romera","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Maurer, F., Naranjo Arcos, M.A., and Bauer, P. (2014). Responses of a triple mutant defective in three iron deficiency-induced BASIC HELIX-LXOOP-HELIX genes of the subgroup Ib(2) to iron deficiency and salicylic acid. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0099234"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1016\/j.plaphy.2011.01.025","article-title":"Suppression of Fe deficiency gene expression by jasmonate","volume":"49","author":"Maurer","year":"2011","journal-title":"Plant Physiol. Biochem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1016\/j.molp.2018.06.005","article-title":"Four IVa bHLH transcription factors are novel interactors of FIT and mediate JA inhibition of iron uptake in Arabidopsis","volume":"11","author":"Cui","year":"2018","journal-title":"Mol. Plant"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1046\/j.1365-313X.2003.01812.x","article-title":"Target genes for OBP3, a Dof transcription factor, include novel basic helix-loop-helix domain proteins inducible by salicylic acid","volume":"35","author":"Kang","year":"2003","journal-title":"Plant J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1111\/tpj.14124","article-title":"Differential regulation and interaction of homoeologous WRKY18 and WRKY40 in Arabidopsis allotetraploids and biotic stress responses","volume":"97","author":"Abeysinghe","year":"2019","journal-title":"Plant J."},{"key":"ref_59","first-page":"1077","article-title":"Plant defense genes are synergistically induced by ethylene and methyl jasmonate","volume":"8","author":"Xu","year":"1994","journal-title":"Plant Cell"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1094\/MPMI-19-1127","article-title":"Inducible overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection","volume":"19","author":"Mei","year":"2006","journal-title":"Mol. Plant Microbe Interact."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/mp\/ssu049","article-title":"Growth-defense tradeoffs in plants: A balancing act to optimize fitness","volume":"7","author":"Huot","year":"2014","journal-title":"Mol. Plant"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"12570","DOI":"10.1038\/ncomms12570","article-title":"Rewiring of jasmonate and phytochrome B signalling uncouples plant growth-defense tradeoffs","volume":"7","author":"Campos","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/mpp.12906","article-title":"Role of salicylic acid glucosyltransferase in balancing growth and defence for optimum plant fitness","volume":"21","author":"Kobayashi","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3389\/fpls.2018.00090","article-title":"Stereoisomers of the bacterial volatile compound 2,3-Butanediol differently elicit systemic defense responses of pepper against multiple viruses in the field","volume":"9","author":"Kong","year":"2018","journal-title":"Front. Plant Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1007\/s00709-018-1327-9","article-title":"The bacterial volatile dimethyl-hexa-decilamine reveals an antagonistic interaction between jasmonic acid and cytokinin in controlling primary root growth of Arabidopsis seedlings","volume":"256","year":"2019","journal-title":"Protoplasma"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/5\/624\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:28:36Z","timestamp":1760174916000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/5\/624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,14]]},"references-count":66,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["plants9050624"],"URL":"https:\/\/doi.org\/10.3390\/plants9050624","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,14]]}}}