{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:52:16Z","timestamp":1790787136875,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T00:00:00Z","timestamp":1597104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Downy mildew of grapevine is one of the most destructive grapevine diseases worldwide. Nowadays, downy mildew control relies almost exclusively on the use of chemical pesticides, including copper products, which are efficient but controversial due to their environmental toxicity. Natural plant protection products have become important solutions in the quest for the sustainable production of food and pest management. However, most biocontrol agents currently on the market, such as biofungicides or elicitors, have a limited efficacy; thus, they cannot replace chemical compounds in full. Our innovation is a natural active substance, which is a lysate of the amoeba Willaertia magna C2c Maky. This active substance is not only able to elicit grapevine defenses, but it also demonstrates direct fungicidal activity against Plasmopara viticola. The efficacy of this new natural substance was demonstrated both in a greenhouse and in a field. The amoeba lysate provided up to 77% protection to grapevine bunches in the field in a natural and safe way.<\/jats:p>","DOI":"10.3390\/plants9081013","type":"journal-article","created":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T10:56:12Z","timestamp":1597143372000},"page":"1013","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A New Active Substance Derived from Lyzed Willaertia magna C2c Maky Cells to Fight Grapevine Downy Mildew"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-9209","authenticated-orcid":false,"given":"Sandrine","family":"Deman\u00e8che","sequence":"first","affiliation":[{"name":"R&amp;D Department, Amo\u00e9ba, 69680 Chassieu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laur\u00e8ne","family":"Mirabel","sequence":"additional","affiliation":[{"name":"R&amp;D Department, Amo\u00e9ba, 69680 Chassieu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Abbe","sequence":"additional","affiliation":[{"name":"R&amp;D Department, Amo\u00e9ba, 69680 Chassieu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Eberst","sequence":"additional","affiliation":[{"name":"R&amp;D Department, Amo\u00e9ba, 69680 Chassieu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Souche","sequence":"additional","affiliation":[{"name":"R&amp;D Department, Amo\u00e9ba, 69680 Chassieu, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,11]]},"reference":[{"key":"ref_1","unstructured":"(2020, April 30). 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