{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T03:55:50Z","timestamp":1781322950595,"version":"3.54.1"},"reference-count":48,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Chinese Academy of Sciences (CAS) President\u2019s International Fellowship Initiative (CAS-PIFI) projects","award":["2018PB0089"],"award-info":[{"award-number":["2018PB0089"]}]},{"name":"The Chinese Academy of Sciences (CAS) President\u2019s International Fellowship Initiative (CAS-PIFI) projects","award":["2019VBA0026"],"award-info":[{"award-number":["2019VBA0026"]}]},{"name":"the Major Project for Special Technology Innovation of Hubei Province","award":["2017AHB054"],"award-info":[{"award-number":["2017AHB054"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Plukenetia huayllabambana is an edible plant traditionally used to cure wounds and various infections. The present work assessed, for the first time, the antibacterial efficacy of solvent fractions from P. huayllabambana fruits. The crude methanol extract was obtained applying ultrasound-assisted extraction, followed by partitioning through successive depletion among solvents of increasing polarity to yield fractions (n-hexane, dichloromethane, ethyl acetate, and n-butanol). The minimal inhibitory concentration (MIC) was determined following antibacterial testing, using the broth microdilution technique against a panel of drug-resistant Gram-negative and Gram-positive bacteria. Possible modes of action of the most active fraction were also investigated. Gas chromatography\u2013mass spectrometry (GC\u2013MS) was used to identify phytocompounds that may account for the recorded activities. Methanol, n-hexane (PH-n-Hex), and ethyl acetate extracts inhibited 100% of studied bacteria, with the recorded MIC ranging from 0.125\u20131 mg\/mL. PH-n-Hex appeared as the most active partition, exerting a bacteriostatic effect. PH-n-Hex probably acts by interfering with bacterial biofilm formation, proton pumps, and bacterial cell membrane integrity. The GC\u2013MS analysis of PH-n-Hex led to the identification of 11 potentially bioactive components, including fatty acids, phytosterol, and diterpene alcohol as major ones. P. huayllabambana can be considered as a plant of pharmacological value\u2014a source of potent anti-infective drug entities.<\/jats:p>","DOI":"10.3390\/plants9091111","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T09:17:08Z","timestamp":1598606228000},"page":"1111","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5501-3111","authenticated-orcid":false,"given":"Armel Jackson","family":"Seukep","sequence":"first","affiliation":[{"name":"CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, P.O. Box 63, Buea, Cameroon"},{"name":"Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minxia","family":"Fan","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4038-0514","authenticated-orcid":false,"given":"Satyajit Dey","family":"Sarker","sequence":"additional","affiliation":[{"name":"Centre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Kuete","sequence":"additional","affiliation":[{"name":"Unit of Research in Microbiology and Antimicrobial Substances\/Laboratory of Cancer Research, Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Quan","family":"Guo","sequence":"additional","affiliation":[{"name":"CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 437004, China"},{"name":"Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,8,28]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization [WHO] (2017). Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections, Including Tuberculosis, World Health Organization. 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