{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:18:20Z","timestamp":1792617500979,"version":"4.5.0"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T00:00:00Z","timestamp":1603065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rural Development Administration of Republic of Korea","award":["PJ01347305"],"award-info":[{"award-number":["PJ01347305"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Sorghum (Sorghum bicolor (L.)) Moench is an important food for humans and feed for livestock. Sorghum contains dhurrin which can be degraded into toxic hydrogen cyanide. Here, we report the expression patterns of 14 candidate genes related to dhurrin ((S)-4-Hydroxymandelnitrile-\u03b2-D-glucopyranoside) metabolism and the effects of the gene expression on specific metabolite content in selected sorghum accessions. Dhurrin-related metabolism is vigorous in the early stages of development of sorghum. The dhurrin contents of most accessions tested were in the range of approximately 6\u201322 \u03bcg mg\u22121 fresh leaf tissue throughout growth. The p-hydroxybenzaldehyde (pHB) contents were high at seedling stages, but almost nonexistent at adult stages. The contents of p-hydroxyphenylacetic acid (pHPAAc) were relatively low throughout growth compared to those of dhurrin or pHB. Generally, the expression of the candidate genes was higher at seedling stage than at other stages and decreased gradually as plants grew. In addition, we identified significant SNPs, and six of them were potentially associated with non-synonymous changes in CAS1. Our results may provide the basis for choosing breeding materials to regulate cyanide contents in sorghum varieties to prevent HCN toxicity of livestock or to promote drought tolerance or pathogen resistance.<\/jats:p>","DOI":"10.3390\/plants9101390","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T20:44:41Z","timestamp":1603140281000},"page":"1390","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Prediction of Dhurrin Metabolism by Transcriptome and Metabolome Analyses in Sorghum"],"prefix":"10.3390","volume":"9","author":[{"given":"Sang Chul","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3121-7600","authenticated-orcid":false,"given":"Yong Suk","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"},{"name":"Department of Plant Resources and Environment, College of Applied Life Sciences, Jeju National University, Jeju 63243, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Gyeong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuna","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Ji","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2157-2246","authenticated-orcid":false,"given":"Sang Un","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3596-2934","authenticated-orcid":false,"given":"Changsoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"},{"name":"Department of Smart Agriculture Systems, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kimber, C.T., Dahlberg, J.A., and Kresovich, S. 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