{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:37:55Z","timestamp":1784043475141,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T00:00:00Z","timestamp":1603152000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002848","name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["FONDECYT\/1170264"],"award-info":[{"award-number":["FONDECYT\/1170264"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["ACM170002"],"award-info":[{"award-number":["ACM170002"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"Comisi\u00f3n Nacional de Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["ANID\/FONDAP\/15130015"],"award-info":[{"award-number":["ANID\/FONDAP\/15130015"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>This work examined the capability of Imperata cylindrica to respond, tolerate and accumulate Cu when growing at high Cu concentration (300 mg kg\u22121 of substrate) at different times of exposure (2, 14 and 21 days). The Cu accumulation in plants was examined by atomic absorption spectroscopy (AAS) and Cu localized by Scanning Electron Microscopy-Energy Dispersive X-Ray spectroscopy. Additionally, the phenolic compound identifications and concentrations were determined using liquid chromatography coupled to mass spectrometry. Our results showed that root biomass decreased significantly at high Cu levels, with a greater decrease at 21 days (39.8% less biomass in comparison to control). The root showed 328 mg Cu kg\u22121 dry weight at 21 days of exposure to Cu, being the tissue that accumulates most of the Cu. Lipid peroxidation was a clear indicator of Cu stress, principally in shoots. The exposure to Cu significantly increased the synthesis of phenolic compounds in shoots of plants exposed 21 days to Cu, where 5-caffeoylquinic acid reached the highest concentrations. Our results support that I. cylindrica is a Cu accumulator plant in root organs with a medium level of accumulation (between 200\u2013600 mg Cu kg\u22121 biomass), which can tolerate the exposure to high Cu levels by means of increasing the synthesis of phenolic compound in shoots, suggesting a potential use as phytoremediation tool in Cu polluted environments.<\/jats:p>","DOI":"10.3390\/plants9101397","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T20:50:07Z","timestamp":1603227007000},"page":"1397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Antioxidant Responses of Phenolic Compounds and Immobilization of Copper in Imperata cylindrica, a Plant with Potential Use for Bioremediation of Cu Contaminated Environments"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8146-3127","authenticated-orcid":false,"given":"Catalina","family":"Vidal","sequence":"first","affiliation":[{"name":"Centro de Investigaci\u00f3n en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8578-3475","authenticated-orcid":false,"given":"Antonieta","family":"Ruiz","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Laboratorio de Biorremediaci\u00f3n, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9658-7752","authenticated-orcid":false,"given":"Giovanni","family":"Larama","sequence":"additional","affiliation":[{"name":"Centro de Modelaci\u00f3n y Computaci\u00f3n Cient\u00edfica, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1398-4195","authenticated-orcid":false,"given":"Rodrigo","family":"Perez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"},{"name":"Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Santander","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5622-6303","authenticated-orcid":false,"given":"Paulo Ademar Avelar","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Solos, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul 97105-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2124-3100","authenticated-orcid":false,"given":"Pablo","family":"Cornejo","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Micorrizas y Sustentabilidad Agroambiental, CIMYSA, Universidad de La Frontera, Avda. Francisco Salazar, 01145 Temuco, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.2134\/jeq2011.0145","article-title":"The Influence of Biochar and Black Carbon on Reduction and Bioavailability of Chromate in Soils","volume":"41","author":"Choppala","year":"2012","journal-title":"J. Environ. Qual."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.scitotenv.2017.10.223","article-title":"Copper immobilization by biochar and microbial community abundance in metal-contaminated soils","volume":"616\u2013617","author":"Moore","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s11104-019-03946-w","article-title":"Alleviation of metal stress by Pseudomonas orientalis and Chaetomium cupreum strains and their effects on Eucalyptus globulus growth promotion","volume":"436","author":"Ortiz","year":"2019","journal-title":"Plant Soil"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.envexpbot.2018.10.017","article-title":"Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism","volume":"161","author":"Berni","year":"2019","journal-title":"Environ. Exp. Bot."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.envexpbot.2019.03.005","article-title":"Efficiency of heavy metals-tolerant plant growth promoting bacteria for alleviating heavy metals toxicity on sorghum","volume":"162","author":"Youssef","year":"2019","journal-title":"Environ. Exp. Bot."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1016\/j.scitotenv.2018.04.268","article-title":"Copper distribution in European topsoils: An assessment based on LUCAS soil survey","volume":"636","author":"Jones","year":"2018","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1089\/ars.2012.5084","article-title":"Copper and Iron Homeostasis in Plants: The Challenges of Oxidative Stress","volume":"19","author":"Ravet","year":"2012","journal-title":"Antioxid. Redox Signal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.scitotenv.2008.07.045","article-title":"Glomalin-related soil protein in a Mediterranean ecosystem affected by a copper smelter and its contribution to Cu and Zn sequestration","volume":"406","author":"Cornejo","year":"2008","journal-title":"Sci. Total Environ."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S0045-6535(99)00385-9","article-title":"Effects of a copper smelter on a grassland community in the Puchuncav\u00ed Valley, Chile","volume":"41","author":"Ginocchio","year":"2000","journal-title":"Chemosphere"},{"key":"ref_10","first-page":"1","article-title":"Heavy Metal Tolerance in Plants: Role of Transcriptomics","volume":"6","author":"Singh","year":"2016","journal-title":"Proteomics"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Ghorbanpour, M., and Varma, A. (2017). The Impact of Drought Stress on the Quality of Spice and Medicinal Plants. Medicinal Plants and Environmental Challenges, Springer.","DOI":"10.1007\/978-3-319-68717-9"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s13765-016-0229-9","article-title":"Responses of the phenolic compounds of Zea mays under heavy metal stress","volume":"59","author":"Kisa","year":"2016","journal-title":"App. Biol. Chem."},{"key":"ref_13","first-page":"523","article-title":"Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress","volume":"15","author":"Michalak","year":"2006","journal-title":"Pol. J. Environ. Stud."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.numecd.2006.12.008","article-title":"Whole grain intake and cardiovascular disease: A meta-analysis","volume":"18","author":"Mellen","year":"2008","journal-title":"Nutr. Metab. Cardiovasc. Dis."},{"key":"ref_15","first-page":"112","article-title":"Antioxidant activity of commonly consumed cereals, millets, pulses and legumes in India","volume":"46","author":"Sreeramulu","year":"2009","journal-title":"Indian J. Biochem. Biophys."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.ecoenv.2017.12.050","article-title":"Interactive effects of aluminum and cadmium on phenolic compounds, antioxidant enzyme activity and oxidative stress in blueberry (Vaccinium corymbosum L.) plantlets cultivated in vitro","volume":"150","author":"Cruces","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jsb.2015.11.005","article-title":"Formation of biomineral iron oxides compounds in a Fe hyperaccumulator plant: Imperata cylindrica (L.) P. Beauv","volume":"193","author":"Fuente","year":"2016","journal-title":"J. Struct. Biol."},{"key":"ref_18","first-page":"1","article-title":"Composition, speciation and distribution of iron minerals in Imperata cylindrica","volume":"20","author":"Amils","year":"2007","journal-title":"Plant Physiol. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/j.1469-8137.2004.01264.x","article-title":"Internal iron biomineralization in Imperata cylindrica, a perennial grass: Chemical composition, speciation and plant localization","volume":"165","author":"Tornero","year":"2005","journal-title":"New Phytol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Wang, Y., and Shen, J.Z. (2018). Identification and Growth Inhibitory Activity of the Chemical Constituents from Imperata Cylindrica Aerial Part Ethyl Acetate Extract. Molecules, 23.","DOI":"10.3390\/molecules23071807"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.ecoenv.2011.08.029","article-title":"Influence of copper on root exudate patterns in some metallophytes and agricultural plants","volume":"75","author":"Meier","year":"2012","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_22","first-page":"173","article-title":"Plant response to Cu toxicity","volume":"40","author":"Tsay","year":"1995","journal-title":"Taiwana"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.tplants.2007.01.004","article-title":"Stress-induced morphogenic responses: Growing out of trouble?","volume":"12","author":"Potters","year":"2007","journal-title":"Trends Plant Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s11270-016-2900-5","article-title":"Black Oat (Avena strigosa Schreb.) Growth and Root Anatomical Changes in Sandy Soil with Different Copper and Phosphorus Concentrations","volume":"227","author":"Ambrosini","year":"2016","journal-title":"Water Air Soil Pollut."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.envpol.2016.05.080","article-title":"Effect of heavy metals and organic matter on root exudates (low molecular weight organic acids) of herbaceous species: An assessment in sand and soil conditions under different levels of contamination","volume":"216","year":"2016","journal-title":"Environ. Pollut."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ecoenv.2018.07.045","article-title":"Growth and chemical changes in the rhizosphere of black oat (Avena strigosa) grown in soils contaminated with copper","volume":"163","author":"Ceretta","year":"2018","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Furini, A. (2012). Plant Responses to Heavy Metal Toxicity. Plants and Heavy Metals, Springer. Springer Briefs in Molecular Science.","DOI":"10.1007\/978-94-007-4441-7"},{"key":"ref_28","first-page":"1","article-title":"Melatonin mitigates cadmium phytotoxicity through modulation of phytochelatins biosynthesis, vacuolar sequestration, and antioxidant potential in Solanum lycopersicum L.","volume":"6","author":"Yin","year":"2015","journal-title":"Front. Plant Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Shanker, A. (2011). Towards understanding plant response to heavy metal stress. Abiotic Stress in Plants\u2014Mechanisms and Adaptations, Tech Europe.","DOI":"10.5772\/895"},{"key":"ref_30","first-page":"279","article-title":"Copper accumulation in a plant community affected by mining contamination in Puchuncav\u00ed valley, central Chile","volume":"81","author":"Muena","year":"2008","journal-title":"Rev. Chil. Hist. Nat."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s11738-006-0009-y","article-title":"Cadmium-induced growth inhibition and alteration of biochemical parameters in almond seedlings grown in solution culture","volume":"29","author":"Nada","year":"2006","journal-title":"Acta Physiol. Plant."},{"key":"ref_32","first-page":"14","article-title":"Contribution of inoculation with arbuscular mycorrhizal fungi to the bioremediation of a copper polluted soil using Oenothera picensis","volume":"17","author":"Cornejo","year":"2017","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.apsoil.2011.04.005","article-title":"Alleviation of Cu toxicity in Oenothera picensis by copper-adapted arbuscular mycorrhizal fungi and treated agrowaste residue","volume":"48","author":"Meier","year":"2011","journal-title":"Appl. Soil Ecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1007\/s11368-015-1224-1","article-title":"Effects of biochar on copper immobilization and soil microbial communities in a metal-contaminated soil","volume":"17","author":"Meier","year":"2015","journal-title":"J. Soils Sediments"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.apsoil.2011.10.018","article-title":"Effects of arbuscular mycorrhizal inoculation on metallophyte and agricultural plants growing at increasing copper levels","volume":"61","author":"Meier","year":"2012","journal-title":"Appl. Soil Ecol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.plaphy.2012.03.008","article-title":"Mercury-induced biochemical and proteomic changes in rice roots","volume":"55","author":"Chen","year":"2012","journal-title":"Plant Physiol. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1590\/S1677-04202009000200003","article-title":"Heavy metals, Co, Ni, Cu, Zn and Cd, produce oxidative damage and evoke differential antioxidant responses in spinach","volume":"21","author":"Pandey","year":"2009","journal-title":"Brazilian J. Plant Physiol."},{"key":"ref_38","first-page":"77","article-title":"Two new chromones and a new flavone glycoside from Imperata cylindrica","volume":"11","author":"Xuan","year":"2013","journal-title":"Chin. J. Nat. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2404","DOI":"10.1002\/jsfa.9447","article-title":"Changes in the content of anthocyanins, flavonols, and antioxidant activity in Fragaria ananassa var. Camarosa fruits under traditional and organic fertilization","volume":"99","author":"Ruiz","year":"2018","journal-title":"J. Sci. Food Agric."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/rcm.6794","article-title":"Low energy induced homolytic fragmentation of flavonol 3- O-glycosides by negative electrospray ionization tandem mass spectrometry","volume":"28","author":"Yang","year":"2014","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"ref_41","first-page":"1","article-title":"Design and evaluation of microencapsulated systems containing extract of whole green coffee fruit rich in phenolic acids","volume":"100","author":"Costa","year":"2020","journal-title":"Food Hydrocoll."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jphotobiol.2018.08.007","article-title":"Eliminating interference by anthocyanins when determining the porphyrin ratio of red plant leaves","volume":"187","author":"Lee","year":"2018","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Aguilera, A., Tereuc\u00e1n, G., Ercoli, S., Cornejo, P., Gomez, R., Uhlmann, L., and Guigas, C. (2020). Influence of Organic and Chemical Fertilization on Antioxidant Compounds Profiles and Activities in Fruits of Fragaria ananassa var. Camarosa. J Soil Sci. Plant Nutr.","DOI":"10.1007\/s42729-019-00159-z"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1046\/j.1469-8137.2002.00482.x","article-title":"Anthocyanins in vegetative tissues: A proposed unified function in photoprotection","volume":"155","author":"Steyn","year":"2002","journal-title":"New Phytol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1016\/j.chemosphere.2013.04.080","article-title":"Growth and photosynthetic responses to copper in wild grapevine","volume":"93","author":"Ocete","year":"2013","journal-title":"Chemosphere"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/j.chemosphere.2014.06.044","article-title":"Evaluating wild grapevine tolerance to copper toxicity","volume":"120","author":"Figueroa","year":"2015","journal-title":"Chemosphere"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1080\/15226514.2019.1633263","article-title":"Characterization of physiochemical and anatomical features associated with enhanced phytostabilization of copper in Bruguiera cylindrica (L.) Blume","volume":"21","author":"Sruthi","year":"2019","journal-title":"Int. J. Phytoremediat."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"110264","DOI":"10.1016\/j.ecoenv.2020.110264","article-title":"Alteration of enzyme activities and functional diversity of a soil contaminated with copper and arsenic","volume":"192","author":"Aponte","year":"2020","journal-title":"Ecotox. Environ. Saf."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1566","DOI":"10.1021\/jf00021a018","article-title":"Modified Thiobarbituric Acid Assay for Measuring Lipid Oxidation in Sugar-Rich Plant Tissue Extracts","volume":"40","author":"Du","year":"1992","journal-title":"J. Agric. Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1002\/jsfa.10166","article-title":"Efficiency of two arbuscular mycorrhizal fungal inocula to improve saline stress tolerance in lettuce plants by changes of antioxidant defense mechanisms","volume":"100","author":"Santander","year":"2020","journal-title":"J. Sci. Food Agric."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1397","DOI":"10.1002\/jsfa.9316","article-title":"Effect of fertilization and arbuscular mycorrhizal fungal inoculation on antioxidant profiles and activities in Fragaria ananassa fruit","volume":"99","author":"Parada","year":"2019","journal-title":"J. Sci. Food Agric."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"144","DOI":"10.5344\/ajev.1965.16.3.144","article-title":"Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents","volume":"16","author":"Singleton","year":"1965","journal-title":"Am. J. Enol. Vitic."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/10\/1397\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:24:56Z","timestamp":1760178296000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/10\/1397"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,20]]},"references-count":52,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2020,10]]}},"alternative-id":["plants9101397"],"URL":"https:\/\/doi.org\/10.3390\/plants9101397","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,20]]}}}