{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:02:51Z","timestamp":1787029371697,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plants"],"abstract":"<jats:p>Satureja montana herbal species belongs to aromatic medicinal plants with a significant place in traditional medicine. However, products produced with conventional procedures do not meet the requirements of the modern market which include environmentally-safe processes that provide quality, safe, and standardized products. In this study, the antiproliferative activity of S. montana extracts obtained by supercritical carbon dioxide and solid\u2013liquid extraction followed by spray drying was investigated using the in vivo model of Ehrlich ascites carcinoma (EAC) in mice. The impact of two concentrations of extracts on the growth of tumor and the redox status of malignant cells was monitored. It was determined that the extracts induced oxidative stress in the malignant cells which was confirmed by the changes in activity of biochemical indicators of oxidative stress. The posttreatment was not an efficient approach, while the extracts applied as pretreatment and treatment resulted in an increase in the xanthine oxidase (XOD) activity, a decrease in catalase (CAT) activity, and an increase in the intensity of lipid peroxidation (LPx). Furthermore, a decrease in the values of reduced glutathione (GSH) and an increase in glutathione reductase (GR) and glutathione peroxidase (GSHPx) in EAC cells were recorded.<\/jats:p>","DOI":"10.3390\/plants9111532","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T14:10:41Z","timestamp":1605017441000},"page":"1532","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of Anticancer Activity of Satureja montana Supercritical and Spray-Dried Extracts on Ehrlich\u2019s Ascites Carcinoma Bearing Mice"],"prefix":"10.3390","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3891-7266","authenticated-orcid":false,"given":"Jelena","family":"Vladi\u0107","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"\u0106ebovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6936-3289","authenticated-orcid":false,"given":"Senka","family":"Vidovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0678-7660","authenticated-orcid":false,"given":"Stela","family":"Joki\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuha\u010da 20, 31000 Osijek, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.1002\/cbdv.201100376","article-title":"Chemotaxonomic and micromorphological traits of Satureja montana L. and S. subspicata Vis. (Lamiaceae)","volume":"9","author":"Kremer","year":"2012","journal-title":"Chem. Biodivers."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1080\/10826070601063559","article-title":"HPLC screening of phenolic compounds in winter savory (Satureja montana L.) extracts","volume":"30","author":"Djilas","year":"2007","journal-title":"J. Liq. Chromatogr. Relat. Technol."},{"key":"ref_3","first-page":"150","article-title":"A review of therapeutic and pharmacological effects of thymol","volume":"8","author":"Parsaei","year":"2016","journal-title":"Der Pharm. Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.1002\/ptr.6103","article-title":"Carvacrol and human health: A comprehensive review","volume":"32","author":"Varoni","year":"2018","journal-title":"Phytother. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1080\/13880200390502180","article-title":"Chemical composition and antidiarrhoeal activities of winter savory (Satureja montana L.) essential oil","volume":"41","year":"2003","journal-title":"Pharm. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1002\/ptr.2650070508","article-title":"Diuretic activity of Satureja montana subsp. montana extracts and oil in rats","volume":"7","author":"Stanic","year":"1993","journal-title":"Phytother. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1248\/bpb.21.829","article-title":"Anti-HIV-1 activity of herbs in Labiatae","volume":"21","author":"Yamasaki","year":"1998","journal-title":"Biol. Pharm. Bull."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1016\/j.foodcont.2005.11.009","article-title":"Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes","volume":"18","author":"Oussalah","year":"2007","journal-title":"Food Control."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4679","DOI":"10.1002\/jsfa.8472","article-title":"The synergistic potential of various teas, herbs and therapeutic drugs in health improvement: A review","volume":"97","author":"Malongane","year":"2017","journal-title":"J. Sci. Food Agric."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S1383-5742(99)00057-5","article-title":"Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances","volume":"428","author":"Surh","year":"1999","journal-title":"Mutat. Res.\/Fundam. Mol. Mech. Mutagenesis"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5757","DOI":"10.21873\/anticanres.11159","article-title":"Antioxidant and antiproliferative properties of the essential oils of Satureja thymbra and Satureja parnassica and their major constituents","volume":"36","author":"Fitsiou","year":"2016","journal-title":"Anticancer Res."},{"key":"ref_12","first-page":"502","article-title":"In vitro Antimicrobial and cytotoxicity assays of Satureja bakhtiarica and Zataria multiflora essential oils","volume":"3","author":"Tahmasbpour","year":"2015","journal-title":"AJPCT"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.3109\/1547691X.2013.789939","article-title":"Toxicity of essential oil of Satureja khuzistanica: In vitro cytotoxicity and anti-microbial activity","volume":"11","author":"Yousefzadi","year":"2014","journal-title":"J. Immunotoxicol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/S0278-6915(99)00075-7","article-title":"Toxicity of selected plant volatiles in microbial and mammalian short-term assays","volume":"37","author":"Stammati","year":"1999","journal-title":"Food Chem. Toxicol."},{"key":"ref_15","first-page":"93","article-title":"Inhibitory effects of carvacrol on DMBA induced pulmonary tumorigenesis in rats","volume":"2","author":"Zeytinoglu","year":"1998","journal-title":"Acta Pharm. Turc."},{"key":"ref_16","first-page":"478","article-title":"Comparative study of DNA-damaging and DNA-protective effects of selected components of essential plant oils in human leukemic cells K562","volume":"54","author":"Horvathova","year":"2006","journal-title":"Neoplasma"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1078\/094471103322004785","article-title":"Inhibition of DNA synthesis by carvacrol in mouse myoblast cells bearing a human N-RAS oncogene","volume":"10","author":"Zeytinoglu","year":"2003","journal-title":"Phytomedicine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s11010-011-1043-7","article-title":"Potential preventive effect of carvacrol against diethylnitrosamine-induced hepatocellular carcinoma in rats","volume":"360","author":"Jayakumar","year":"2012","journal-title":"Mol. Cell. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.supflu.2017.05.025","article-title":"Antioxidative and cytotoxic activity of essential oils and extracts of Satureja montana L., Coriandrum sativum L. and Ocimum basilicum L. obtained by supercritical fluid extraction","volume":"128","author":"Elgndi","year":"2017","journal-title":"J. Supercrit. Fluids"},{"key":"ref_20","first-page":"443","article-title":"Antioxidative and antiproliferative effects of Satureja montana L. extracts","volume":"9","author":"Tumbas","year":"2004","journal-title":"J. BUON"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1002\/jssc.200800490","article-title":"Enrichment of the thymoquinone content in volatile oil from Satureja montana using supercritical fluid extraction","volume":"32","author":"Grosso","year":"2009","journal-title":"J. Sep. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"C713","DOI":"10.1111\/j.1750-3841.2009.01376.x","article-title":"Antioxidant activities of the supercritical and conventional Satureja montana extracts","volume":"74","author":"Grosso","year":"2009","journal-title":"J. Food Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.2298\/CICEQ150504034D","article-title":"Effect of matrix pretreatment on the supercritical CO2 extraction of Satureja montana essential oil","volume":"22","author":"Lu","year":"2016","journal-title":"Chem. Ind. Chem. Eng. Q."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1016\/j.supflu.2014.10.019","article-title":"Influence of pre-treatments on yield, chemical composition and antioxidant activity of Satureja montana extracts obtained by supercritical carbon dioxide","volume":"95","year":"2014","journal-title":"J. Supercrit. Fluids"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.supflu.2016.10.016","article-title":"Optimization of Satureja montana subcritical water extraction process and chemical characterization of volatile fraction of extracts","volume":"120","author":"Canli","year":"2017","journal-title":"J. Supercrit. Fluids"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Jakovljevi\u0107, M., Vladi\u0107, J., Vidovi\u0107, S., Pastor, K., Joki\u0107, S., Molnar, M., and Jerkovi\u0107, I. (2020). Application of deep eutectic solvents for the extraction of rutin and rosmarinic acid from Satureja montana L. and evaluation of the extracts antiradical activity. Plants, 9.","DOI":"10.3390\/plants9020153"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3136\/fstr.14.311","article-title":"Use of microwave-assisted hydrodistillation to extract the essential oils from Satureja hortensis and Satureja montana","volume":"14","author":"Rezvanpanah","year":"2008","journal-title":"Food Sci. Technol. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1080\/01496395.2017.1288744","article-title":"Optimization: Microwave irradiation effect on polyphenolic compounds extraction from winter savory (Satureja montana L.)","volume":"52","author":"Zekovic","year":"2017","journal-title":"Sep. Sci. Technol."},{"key":"ref_29","first-page":"907","article-title":"Pharmaceutical applications of supercritical carbon dioxide","volume":"56","author":"Kaiser","year":"2001","journal-title":"Die Pharm."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/j.foodres.2007.07.004","article-title":"Applications of spray-drying in microencapsulation of food ingredients: An overview","volume":"40","author":"Gharsallaoui","year":"2007","journal-title":"Food Res. Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.indcrop.2015.11.085","article-title":"Recycling of filter tea industry by-products: Production of A. millefolium powder using spray drying technique","volume":"80","author":"Ambrus","year":"2016","journal-title":"Ind. Crop. Prod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.supflu.2016.05.027","article-title":"Winter savory: Supercritical carbon dioxide extraction and mathematical modeling of extraction process","volume":"117","year":"2016","journal-title":"J. Supercrit. Fluids"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.powtec.2014.03.038","article-title":"Maltodextrin as a carrier of health benefit compounds in Satureja montana dry powder extract obtained by spray drying technique","volume":"258","year":"2014","journal-title":"Powder Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.cbi.2014.11.001","article-title":"Protective effect of Satureja montana extract on cyclophosphamide-induced testicular injury in rats","volume":"224","author":"Shahin","year":"2014","journal-title":"Chem. Biol. Interact."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"11557","DOI":"10.1021\/jf901786p","article-title":"Phytochemical profile and anticholinesterase and antimicrobial activities of supercritical versus conventional extracts of Satureja montana","volume":"57","author":"Silva","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_36","first-page":"5","article-title":"Assessment of antioxidant and hepatoprotective potential of Satureja montana extracts against CCl4 induced liver damage","volume":"39","year":"2019","journal-title":"Nat. Med. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1554","DOI":"10.1002\/jsfa.4347","article-title":"Antioxidant and antimicrobial activity of Satureja montana L. extracts","volume":"91","author":"Serrano","year":"2011","journal-title":"J. Sci. Food Agric."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ipek, E., T\u00fcyl\u00fc, B.A., and Zeytinoglu, H. (2003). Effects of carvacrol on sister chromatid exchanges in human lymphocyte cultures. Animal Cell Technology: Basic & Applied Aspects, Springer.","DOI":"10.1007\/978-94-017-0726-8_81"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/j.phymed.2009.12.008","article-title":"Anti-proliferative effects of carvacrol on a human metastatic breast cancer cell line, MDA-MB 231","volume":"17","author":"Arunasree","year":"2010","journal-title":"Phytomedicine"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s10616-011-9389-y","article-title":"Anti-proliferative and pro-apoptotic effect of carvacrol on human hepatocellular carcinoma cell line HepG-2","volume":"64","author":"Yin","year":"2012","journal-title":"Cytotechnology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1924","DOI":"10.1016\/j.fct.2011.04.007","article-title":"Antitumor properties of Boswellic acid against Ehrlich ascites cells bearing mouse","volume":"49","author":"Agrawal","year":"2011","journal-title":"Food Chem. Toxicol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1002\/ptr.2464","article-title":"Cytotoxic effects of the Viscum album L. extract on Ehrlich tumour cells in vivo","volume":"22","year":"2008","journal-title":"Phytother. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1039\/c3fo30315b","article-title":"Antiradical, antimicrobial and cytotoxic activities of commercial beetroot pomace","volume":"4","author":"Djilas","year":"2013","journal-title":"Food Funct."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1021\/jf9908188","article-title":"Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical","volume":"48","author":"Wichers","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Bergmayer, U.H. (1970). Methoden der Enzymatischen Analyse, Chemie.","DOI":"10.1515\/9783112719336"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0021-9258(19)50881-X","article-title":"Spectrophotometric method for measuring of breakdown of hydrogen peroxide by catalase","volume":"195","author":"Beers","year":"1950","journal-title":"J. Biol. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0015-3796(17)30073-2","article-title":"Study of metabolism enzymes during the development of Phaseolus vulgaris","volume":"166","author":"Simon","year":"1974","journal-title":"Biochem. Physiol. Pflanz."},{"key":"ref_48","unstructured":"Beutler, E. (1984). Glutathione reductase, glutathione peroxidase, catalase, glutathione. Red Cell Metabolism: A Manual of Biochemical Methods, Grune and Stratton."},{"key":"ref_49","unstructured":"Bergmayer, H.U. (1983). Glutathione reductase. Methods of Enzymatic Analysis, Chemie."},{"key":"ref_50","first-page":"882","article-title":"Improved methods for the determination of blood glutathione","volume":"61","author":"Beuthler","year":"1983","journal-title":"J. Lab. Clin. Med."},{"key":"ref_51","unstructured":"Fleisher, S., and Parker, L. (1978). Microsomal lipid peroxidation. Methods in Enzymology, Academic Press."}],"container-title":["Plants"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/11\/1532\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:31:44Z","timestamp":1760178704000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2223-7747\/9\/11\/1532"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,10]]},"references-count":51,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,11]]}},"alternative-id":["plants9111532"],"URL":"https:\/\/doi.org\/10.3390\/plants9111532","relation":{},"ISSN":["2223-7747"],"issn-type":[{"value":"2223-7747","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,10]]}}}