{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T17:05:56Z","timestamp":1778605556816,"version":"3.51.4"},"reference-count":22,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T00:00:00Z","timestamp":1518652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p> Molecularly imprinted thin layers were prepared in silica capillaries by using two different surface polymerization strategies, the first using 4,4\u2032-azobis(4-cyanovaleric acid) as a surface-coupled radical initiator, and the second, S-carboxypropyl-S\u2019-benzyltrithiocarbonate as a reversible addition-fragmentation chain transfer (RAFT) agent in combination with 2,2\u2032-azobisisobutyronitrile as a free radical initiator. The ability to generate imprinted thin layers was tested on two different polymerization systems: (i) a 4-vinylpyridine\/ethylene dimethacrylate (4VP-EDMA) in methanol-water solution with 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) as a template; and (ii) methacrylic acid\/ethylene dimethacrylate (MAA-EDMA) in a chloroform solution with warfarin as the template molecule. The binding properties of the imprinted capillaries were studied and compared with those of the corresponding non-imprinted polymer coated capillaries by injecting the template molecule and by measuring its migration times relative to a neutral and non-retained marker. The role of running buffer hydrophobicity on recognition was investigated by studying the influence of varying buffer acetonitrile concentration. The 2,4,5-T-imprinted capillary showed molecular recognition based on a reversed phase mechanism, with a decrease of the template recognition in the presence of higher acetonitrile content; whereas warfarin-imprinted capillaries showed a bell-shaped trend upon varying the acetonitrile percentage, illustrating different mechanisms underlying imprinted polymer-ligand recognition. Importantly, the results demonstrated the validity of affinity capillary electrochromatography (CEC) to screen the binding properties of imprinted layers.<\/jats:p>","DOI":"10.3390\/polym10020192","type":"journal-article","created":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T03:54:22Z","timestamp":1519098862000},"page":"192","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Affinity Capillary Electrochromatography of Molecularly Imprinted Thin Layers Grafted onto Silica Capillaries Using a Surface-Bound Azo-Initiator and Living Polymerization"],"prefix":"10.3390","volume":"10","author":[{"given":"Cristina","family":"Giovannoli","sequence":"first","affiliation":[{"name":"Laboratory of Bioanalytical Chemistry, Department of Chemistry, Via Giuria 7, University of Torino, 10125, Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cinzia","family":"Passini","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalytical Chemistry, Department of Chemistry, Via Giuria 7, University of Torino, 10125, Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Di","family":"Nardo","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalytical Chemistry, Department of Chemistry, Via Giuria 7, University of Torino, 10125, Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2920-0140","authenticated-orcid":false,"given":"Laura","family":"Anfossi","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalytical Chemistry, Department of Chemistry, Via Giuria 7, University of Torino, 10125, Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1024-2532","authenticated-orcid":false,"given":"Claudio","family":"Baggiani","sequence":"additional","affiliation":[{"name":"Laboratory of Bioanalytical Chemistry, Department of Chemistry, Via Giuria 7, University of Torino, 10125, Torino, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0407-6542","authenticated-orcid":false,"given":"Ian A.","family":"Nicholls","sequence":"additional","affiliation":[{"name":"Bioorganic & Biophysical Chemistry Laboratory, Centre for Biomaterials Chemistry, Department of Chemistry & Biomedical Sciences, Linnaeus University, SE-39182 Kalmar, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,2,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1039\/C6CS00061D","article-title":"Molecular imprinting: Perspectives and applications","volume":"45","author":"Chen","year":"2016","journal-title":"Chem. 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