{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:52:20Z","timestamp":1760550740136,"version":"build-2065373602"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2019,1,6]],"date-time":"2019-01-06T00:00:00Z","timestamp":1546732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005632","name":"The National Centre for Research and Development","doi-asserted-by":"publisher","award":["LIDER\/154\/L-6\/14\/NCBR\/2015."],"award-info":[{"award-number":["LIDER\/154\/L-6\/14\/NCBR\/2015."]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Polymers modified with bioactive nanoparticles are a promising solution for patients who need a tissue replacement. Modern implants, thanks to bioactive and bactericidal functions, facilitate the healing and regeneration process of the replaced tissue. The aim of this study was to assess whether silver nanoparticles (AgNPs) could support antibacterial function without cytotoxic effect and deterioration of biostability. This article describes biological and physiochemical aspects concerning a new polymeric middle ear implant (Otoimplant) enriched with silver nanoparticles. This kind of prosthesis is a promising implant for the reconstruction of ossicles in ossiculoplasty. We found that incorporation of silver nanoparticles into a polymeric matrix resulted in bactericidal efficacy against Gram-positive and Gram-negative bacteria, both resistant to antibiotics and basic strains. Our prostheses do not show cytotoxic effect and are a suitable biomaterial platform for effective culture of Saos2 and NHOst osteoblastic cells. The in vitro incubation of the samples in distilled water revealed that surface parameters, such as roughness, may slightly increase as a result of unveiling nanoparticles. However, the prolonged immersion does not change mechanical parameters. During one-year incubation, the prosthesis proved to retain stable values of Young\u2019s modulus, tensile strength, propagation of longitudinal ultrasonic waves, pH, and conductivity.<\/jats:p>","DOI":"10.3390\/polym11010079","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T03:06:06Z","timestamp":1547003166000},"page":"79","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Biological and Physicochemical Assessment of Middle Ear Prosthesis"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0702-7137","authenticated-orcid":false,"given":"Magdalena","family":"Zi\u0105bka","sequence":"first","affiliation":[{"name":"AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Ceramics and Refractories, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5661-5467","authenticated-orcid":false,"given":"Micha\u0142","family":"Dziadek","sequence":"additional","affiliation":[{"name":"AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Glass Technology and Amorphous Coatings, 30-059 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Kr\u00f3licka","sequence":"additional","affiliation":[{"name":"University of Gdansk, Intercollegiate Faculty of Biotechnology UG-GUMed, Department of Biotechnology, Laboratory of Biologically Active Compounds, 80-307 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,1,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.12998\/wjcc.v3.i1.52","article-title":"Implant biomaterials: A comprehensive review","volume":"16","author":"Saini","year":"2015","journal-title":"World J. Clin. Cases"},{"key":"ref_2","first-page":"260","article-title":"Tragal Cartilage versus Polytetrafluoroethylene (TEFLON) Partial Ossicular Replacement Prosthesis (PORP): A Comparative Study of Outcomes of Ossiculoplasty","volume":"1","author":"Pathan","year":"2016","journal-title":"Int. J. Innov. Res. Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1203\/01.pdr.0000305937.26105.e7","article-title":"Polymeric Biomaterials in Tissue Engineering","volume":"63","author":"Kohane","year":"2008","journal-title":"Pediatr. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1001\/archoto.2012.637","article-title":"Bacterial Biofilm Associated with Chronic Laryngitis","volume":"138","author":"Kinnari","year":"2012","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1177\/0194599812472297","article-title":"Surgical Site Infections in Ear Surgery: Hair Removal Effect; a Preliminary, Randomized Trial Study","volume":"148","author":"Kattipattanapong","year":"2013","journal-title":"Otolaryngol. Head Neck Surg."},{"key":"ref_6","first-page":"433","article-title":"The Differential Diagnosis of Hearing Loss","volume":"108","author":"Zahnert","year":"2011","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_7","first-page":"8","article-title":"Symptom: Congenital Conductive Hearing Loss","volume":"67","author":"Djalilian","year":"2014","journal-title":"Hear. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s40136-017-0152-6","article-title":"Streptococcus pneumoniae Otitis Media Pathogenesis and How It Informs Our Understanding of Vaccine Strategies","volume":"5","author":"Bergenfelz","year":"2017","journal-title":"Curr. Otorhinolaryngol. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1111\/2049-632X.12129","article-title":"Residence of Streptococcus pneumoniae and Moraxella catarrhalis within polymicrobial biofilm promotes antibiotic resistance and bacterial persistence in vivo","volume":"70","author":"Perez","year":"2014","journal-title":"Pathog. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Angoulvant, F., Cohen, R., Doit, C., Elbez, A., Werner, A., B\u00e9chet, S., Bonacorsi, S., Varon, E., and Levy, C. (2015). Trends in antibiotic resistance of Streptococcus pneumoniae and Haemophilus influenzae isolated from nasopharyngeal flora in children with acute otitis media in France before and after valent pneumococcal conjugate vaccine introduction. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-0978-9"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.2147\/IJN.S121956","article-title":"The antimicrobial activity of nanoparticles: Present situation and prospects for the future","volume":"12","author":"Wang","year":"2017","journal-title":"Int. J. Nanomed."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.jconrel.2011.07.002","article-title":"Nanoantibiotics: A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era","volume":"156","author":"Huh","year":"2011","journal-title":"J. Controll."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1179\/016164107X229902","article-title":"Silver segregation and bacterial growth of intraventricular catheters impregnated with silver nanoparticles in cerebrospinal fluid drainages","volume":"30","author":"Galiano","year":"2008","journal-title":"Neurol. Res."},{"key":"ref_14","first-page":"4131","article-title":"Silver nanoparticle\/chitosan oligosaccharide\/poly (vinylalcohol) nanofibers as wound dressings: A preclinical study","volume":"8","author":"Li","year":"2013","journal-title":"Int. J. Nanomed."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"055014","DOI":"10.1088\/1748-6041\/10\/5\/055014","article-title":"Antibacterial and bioactive composite bone cements containing surface silver-doped glass particles","volume":"10","author":"Miola","year":"2015","journal-title":"Biomed. Mater."},{"key":"ref_16","first-page":"200","article-title":"Comparison of antibacterial effects of ZnO and CuO nanoparticles coated brackets against Streptococcus mutans","volume":"16","author":"Ramazanzadeh","year":"2015","journal-title":"J. Dent."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1002\/adhm.201100032","article-title":"Mitigation of Staphylococcus aureus\u2014Mediated Surgical Site Infections with IR Photoactivated TiO2 coatings on Ti Implants","volume":"1","author":"Aboelzahab","year":"2012","journal-title":"Adv. Health Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1007\/s12070-011-0373-9","article-title":"The Efficiency of Titanium Middle Ear Prosthesis in Ossicular Chain Reconstruction: Our Experience","volume":"65","author":"Shah","year":"2013","journal-title":"Indian J. Otolaryngol. Head Neck Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1097\/MAO.0b013e318064e8e8","article-title":"Audiometric outcomes of tympanoplasty with hydroxylapatite prosthesis: Consultant versus trainees","volume":"28","author":"Charlett","year":"2007","journal-title":"Otol. Neurotol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1097\/mao.0b013e31815c2575","article-title":"Titanium CliP piston versus platinum-ribbon Teflon piston: Piston and fenestra size affect air-bone gap","volume":"29","author":"Mangham","year":"2008","journal-title":"Otol. Neurotol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zi\u0105bka, M., Dziadek, M., Menaszek, E., Banasiuk, R., and Kr\u00f3licka, A. (2017). Middle ear prosthesis with bactericidal efficacy\u2014In vitro investigation. Molecules, 22.","DOI":"10.3390\/molecules22101681"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zi\u0105bka, M., Menaszek, E., Tarasiuk, J., and Wro\u0144ski, S. (2018). Biocompatible Nanocomposite Implant with Silver Nanoparticles for Otology\u2014In Vivo Evaluation. Nanomaterials, 8.","DOI":"10.3390\/nano8100764"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zi\u0105bka, M., Dziadek, M., and Menaszek, E. (2018). Biocompatibility of Poly(acrylonitrile-butadiene-styrene) Nanocomposites Modified with Silver Nanoparticles. Polymers, 10.","DOI":"10.3390\/polym10111257"},{"key":"ref_24","first-page":"315","article-title":"Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment","volume":"11","author":"Banasiuk","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_25","unstructured":"PN-EN (2010). ISO 10993-13:2010 Biological Evaluation of Medical Devices\u2014Part 13: Identification and Quantification of Degradation Products from Polymeric Medical Devices, International Organization for Standardization."},{"key":"ref_26","unstructured":"PN-EN (2012). ISO 527-1:2012 Plastics\u2014Determination of Tensile Properties\u2014Part 1: General Principles, International Organization for Standardization."},{"key":"ref_27","unstructured":"ASTM International (2012). ASTM E 2180\u201407 Norm (Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials), ASTM International."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"114","DOI":"10.5604\/01.3001.0012.5169","article-title":"Surface Properties of Polymeric Composites with Silver Nanoparticles","volume":"26","author":"Dziadek","year":"2018","journal-title":"Fibres Text. East. Eur."},{"key":"ref_29","first-page":"119","article-title":"Synthesis and characterization of silver nanoparticles for antibacterial activity","volume":"1","author":"Sadeghi","year":"2010","journal-title":"Int. J. Nano Dimen."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1002\/masy.201250527","article-title":"High Density Polyethylene Containing Antibacterial Silver Nanoparticles for Medical Applications","volume":"315","author":"Mertas","year":"2012","journal-title":"Macromol. Symp."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1016\/S0142-9612(00)00274-X","article-title":"Effect of surface roughness of the titanium alloy Ti\u20136Al\u20134V on human bone marrow cell response and on protein adsorption","volume":"22","author":"Deligianni","year":"2001","journal-title":"Biomaterials"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1016\/S0142-9612(02)00379-4","article-title":"Response of rat osteoblast-like cells to microstructured model surfaces in vitro","volume":"24","author":"Liao","year":"2003","journal-title":"Biomaterials"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1177\/154405910608500603","article-title":"Implant surface roughness and bone healing: A systematic review","volume":"85","author":"Shalabi","year":"2006","journal-title":"J. Dent. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1243\/09544119JEIM901","article-title":"Relative influence of surface topography and surface chemistry on cell response to bone implant materials. Part 2: Biological aspects","volume":"224","author":"Anselme","year":"2010","journal-title":"Proc. Inst. Mech. Eng. Part. H J. Eng. Med."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1179\/1743280414Y.0000000038","article-title":"The role of surface free energy in osteoblast\u2013biomaterial interactions","volume":"59","author":"Gentleman","year":"2014","journal-title":"Int. Mater. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15029","DOI":"10.1038\/boneres.2015.29","article-title":"Nanomaterials and bone regeneration","volume":"3","author":"Gong","year":"2015","journal-title":"Bone Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.bone.2004.03.009","article-title":"Roughness response genes in osteoblasts","volume":"35","author":"Brett","year":"2004","journal-title":"Bone"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1856","DOI":"10.1021\/bm100374n","article-title":"Synergistically Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells by Culture on Nanostructured Surfaces with Induction Media","volume":"11","author":"You","year":"2010","journal-title":"Biomacromolecules"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/11\/1\/79\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:23:53Z","timestamp":1760185433000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/11\/1\/79"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,6]]},"references-count":38,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,1]]}},"alternative-id":["polym11010079"],"URL":"https:\/\/doi.org\/10.3390\/polym11010079","relation":{},"ISSN":["2073-4360"],"issn-type":[{"type":"electronic","value":"2073-4360"}],"subject":[],"published":{"date-parts":[[2019,1,6]]}}}