{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T17:35:44Z","timestamp":1786469744020,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,13]],"date-time":"2020-02-13T00:00:00Z","timestamp":1581552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Using N,N-dimethylacetamide (DMAc) as a reducing agent in the presence of PVP-K30, the stable silver nanoparticles (Ag-NPs) solution was prepared by a convenient method for the in situ reduction of silver nitrate. The cellulose\u2013Ag-NPs composite film (CANF) was cast in the same container using lithium chloride (LiCl) giving the Ag-NPs-PVP\/DMAc solution cellulose solubility as well as \u03b3-mercaptopropyltrimethoxysilane (MPTS) to couple Ag-NPs and cellulose. The results showed that the Ag-NPs were uniformly dispersed in solution, and the solution had strong antibacterial activities. It was found that the one-pot synthesis allowed the growth of and cross-linking with cellulose processes of Ag-NPs conducted simultaneously. Approximately 61% of Ag-NPs was successfully loaded in CANF, and Ag-NPs were uniformly dispersed in the surface and internal of the composite film. The composite film exhibited good tensile properties (tensile strength could reach up to 86.4 MPa), transparency (light transmittance exceeds 70%), thermal stability, and remarkable antibacterial activities. The sterilization effect of CANF0.04 against Staphylococcus aureus and Escherichia coli exceed 99.9%. Due to low residual LiCl\/DMAc and low diffusion of Ag-NPs, the composite film may have potential for applications in food packaging and bacterial barrier.<\/jats:p>","DOI":"10.3390\/polym12020440","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T08:12:22Z","timestamp":1582618342000},"page":"440","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["A One-Pot Synthesis and Characterization of Antibacterial Silver Nanoparticle\u2013Cellulose Film"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2244-3561","authenticated-orcid":false,"given":"Qi-Yuan","family":"Chen","sequence":"first","affiliation":[{"name":"College of Engineering and Technology, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Ling","family":"Xiao","sequence":"additional","affiliation":[{"name":"College of Engineering and Technology, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheldon Q.","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Energy Engineering, University of North Texas, Denton, TX 76203, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Ping","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Energy Engineering, University of North Texas, Denton, TX 76203, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1007\/s11051-010-9900-y","article-title":"A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment","volume":"12","author":"Hoek","year":"2010","journal-title":"J. Nanopart. Res."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ivask, A., Kurvet, I., Kasemets, K., Blinova, I., Aruoja, V., Suppi, S., Vija, H., K\u00e4kinen, A., Titma, T., and Heinlaan, M. (2014). Size-Dependent Toxicity of Silver Nanoparticles to Bacteria, Yeast, Algae, Crustaceans and Mammalian Cells In Vitro. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0102108"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.3109\/17435390.2010.483745","article-title":"Impact of silver nanoparticles on human cells: Effect of particle size","volume":"4","author":"Liu","year":"2010","journal-title":"Nanotoxicology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1743-8977-11-11","article-title":"Size-dependent cytotoxicity of silver nanoparticles in human lung cells: The role of cellular uptake, agglomeration and Ag release","volume":"11","author":"Gliga","year":"2014","journal-title":"Part. Fibre Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/j.nano.2015.11.016","article-title":"Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity","volume":"12","author":"Seabra","year":"2016","journal-title":"Nanomed. Nanotechnol. Biol. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1080\/17458080.2014.985750","article-title":"Antibacterial mechanism of biogenic silver nanoparticles of Lactobacillus acidophilus","volume":"10","author":"Rajesh","year":"2015","journal-title":"J. Exp. Nanosci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/1574-6968.12460","article-title":"Effect of silver nanoparticles and silver ions on growth and adaptive response mechanisms of Pseudomonas putida mt-2","volume":"355","author":"Hachicho","year":"2014","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1002\/jobm.201300457","article-title":"Interaction of silver nanoparticles with Escherichia coli and their cell envelope biomolecules","volume":"54","author":"Ansari","year":"2014","journal-title":"J. Basic Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1007\/s12275-014-4113-1","article-title":"Evaluation of the antimicrobial potency of silver nanoparticles biosynthesized by using an endophytic fungus, Cryptosporiopsis ericae PS4","volume":"52","author":"Devi","year":"2014","journal-title":"J. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/lam.12015","article-title":"Sequential interactions of silver\u2013silica nanocomposite (Ag\u2013SiO2NC) with cell wall, metabolism and genetic stability of Pseudomonas aeruginosa, a multiple antibiotic-resistant bacterium","volume":"56","author":"Anas","year":"2013","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.jiec.2015.04.005","article-title":"Biologically synthesized silver nanoparticles enhances antibiotic activity against Gram-negative bacteria","volume":"29","author":"Gurunathan","year":"2015","journal-title":"J. Ind. Eng. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1002\/jat.2792","article-title":"Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues","volume":"33","author":"Kim","year":"2013","journal-title":"J. Appl. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.arabjc.2014.10.036","article-title":"Biogenic silver nanoparticles synthesized with rhamnogalacturonan gum: Antibacterial activity, cytotoxicity and its mode of action","volume":"11","author":"Kora","year":"2018","journal-title":"Arab. J. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1056","DOI":"10.1002\/ppsc.201300165","article-title":"Single-Cell Investigations of Silver Nanoparticle\u2013Bacteria Interactions","volume":"30","author":"Vishnupriya","year":"2013","journal-title":"Part. Part. Syst. Charact."},{"key":"ref_15","first-page":"4","article-title":"Some nanoparticles effects on Proteus sp. and Klebsiella sp. isolated from water","volume":"2","author":"Ouda","year":"2014","journal-title":"Am. J. Infect. Dis. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.cis.2008.09.002","article-title":"Silver nanoparticles: Green synthesis and their antimicrobial activities","volume":"145","author":"Sharma","year":"2009","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1007\/s00253-009-2159-5","article-title":"Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli","volume":"85","author":"Li","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1002\/smll.200700954","article-title":"Analysis of the Toxic Mode of Action of Silver Nanoparticles Using Stress-Specific Bioluminescent Bacteria","volume":"4","author":"Hwang","year":"2008","journal-title":"Small"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.jallcom.2018.03.159","article-title":"Enhanced blue photoluminescence of B2O3-CaF2 glass-ceramics containing silver nanoparticles","volume":"749","author":"Naranjo","year":"2018","journal-title":"J. Alloy Compd."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"171113","DOI":"10.1098\/rsos.171113","article-title":"Silver nanoparticles as a medical device in healthcare settings: A five-step approach for candidate screening of coating agents","volume":"5","author":"Marassi","year":"2018","journal-title":"R. Soc. Open Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.impact.2018.02.002","article-title":"Effect of silver concentration and chemical transformations on release and antibacterial efficacy in silver-containing textiles","volume":"11","author":"Zaikova","year":"2018","journal-title":"NanoImpact"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.polymertesting.2017.10.023","article-title":"Antibacterial properties of films of cellulose composites with silver nanoparticles and antibiotics","volume":"65","author":"Volova","year":"2018","journal-title":"Polym. Test."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1080\/00914037.2017.1342247","article-title":"Synthesis, characterization, and antibacterial property of eco-friendly Ag\/cellulose nanocomposite film","volume":"67","author":"Zhai","year":"2018","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/j.biotechadv.2011.05.005","article-title":"Biomass pretreatment: Fundamentals toward application","volume":"29","author":"Agbor","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.fpsl.2018.06.012","article-title":"Antimicrobial poly(lactic acid)\/cellulose bionanocomposite for food packaging application: A review","volume":"17","author":"Gan","year":"2018","journal-title":"Food Packag. Shelf Life"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1007\/s10570-013-9870-1","article-title":"Antimicrobial porous hybrids consisting of bacterial nanocellulose and silver nanoparticles","volume":"20","author":"Berndt","year":"2013","journal-title":"Cellulose"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1016\/j.carbpol.2011.08.079","article-title":"Antimicrobial activity of silver nanoparticle impregnated bacterial cellulose membrane: Effect of fermentation carbon sources of bacterial cellulose","volume":"87","author":"Yang","year":"2012","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1016\/j.carbpol.2013.10.093","article-title":"In situ synthesis of silver-nanoparticles\/bacterial cellulose composites for slow-released antimicrobial wound dressing","volume":"102","author":"Wu","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.memsci.2008.07.010","article-title":"Effect of filler incorporation route on the properties of polysulfone\u2013silver nanocomposite membranes of different porosities","volume":"325","author":"Taurozzi","year":"2008","journal-title":"J. Membr. Sci."},{"key":"ref_30","first-page":"2150","article-title":"Review of Silver Nanoparticles (AgNPs)-Cellulose Antibacterial Composites","volume":"13","author":"Xu","year":"2018","journal-title":"BioResources"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8943","DOI":"10.15376\/biores.13.4.8943-8955","article-title":"Isolation of Cellulose from Poplar Wood by Nitric Acid-Ethanol Treatment and Its Effect on the Quality of Films Cast from Ionic Liquid","volume":"13","author":"Chen","year":"2018","journal-title":"BioResources"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1021\/ma00154a010","article-title":"Solution studies of cellulose in lithium chloride and N,N-dimethylacetamide","volume":"18","author":"McCormick","year":"1985","journal-title":"Macromolecules"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1021\/la980984u","article-title":"Formation and Stabilization of Silver Nanoparticles through Reduction by N,N-Dimethylformamide","volume":"15","year":"1999","journal-title":"Langmuir"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1016\/j.synthmet.2011.04.018","article-title":"Spontaneous formation of highly dispersed spheroidal metallic silver nanoparticles in surfactant-free N,N-dimethylacetamide","volume":"161","author":"Formiga","year":"2011","journal-title":"Synth. Met."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1007\/s10853-008-3204-y","article-title":"Synthesis and electrical properties of uniform silver nanoparticles for electronic applications","volume":"44","author":"Chen","year":"2009","journal-title":"J. Mater. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1002\/adfm.200801566","article-title":"N,N-Dimethylformamide as a Reaction Medium for Metal Nanoparticle Synthesis","volume":"19","year":"2009","journal-title":"Adv. Funct. Mater."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Pham, X.-H., Hahm, E., Kim, H.-M., Shim, S., Kim, T.H., Jeong, D.H., Lee, Y.-S., and Jun, B.-H. (2016). Silver Nanoparticle-Embedded Thin Silica-Coated Graphene Oxide as an SERS Substrate. Nanomaterials, 6.","DOI":"10.3390\/nano6100176"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"(2009). Cellulose modified fibres in cement based composites. Compos. Part A Appl. Sci. Manuf., 40, 2046\u20132053.","DOI":"10.1016\/j.compositesa.2009.09.016"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0169-4332(98)00920-9","article-title":"Evaluation of surface lignin on cellulose fibers with XPS","volume":"144\u2013145","author":"Johansson","year":"1999","journal-title":"Appl. Surf. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1039\/C5GC02576A","article-title":"Facile extraction of cellulose nanocrystals from wood using ethanol and peroxide solvothermal pretreatment followed by ultrasonic nanofibrillation","volume":"18","author":"Li","year":"2016","journal-title":"Green Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.jfoodeng.2011.10.030","article-title":"Development of cellulose-based bactericidal nanocomposites containing silver nanoparticles and their use as active food packaging","volume":"109","author":"Mattoso","year":"2012","journal-title":"J. Food Eng."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Rac-Rumijowska, O., Maliszewska, I., Fiedot-Tobo\u0142a, M., Karbownik, I., and Teterycz, H. (2019). Multifunctional Nanocomposite Cellulose Fibers Doped in Situ with Silver Nanoparticles. Polymers, 11.","DOI":"10.3390\/polym11030562"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1103\/PhysRevB.54.2249","article-title":"Interaction of oxygen with silver at high temperature and atmospheric pressure: A spectroscopic and structural analysis of a strongly bound surface species","volume":"54","author":"Bao","year":"1996","journal-title":"Phys. Rev. B"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"225103","DOI":"10.1088\/0957-4484\/18\/22\/225103","article-title":"Characterization of enhanced antibacterial effects of novel silver nanoparticles","volume":"18","author":"Shrivastava","year":"2007","journal-title":"Nanotechnology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"415","DOI":"10.15376\/biores.15.1.415-428","article-title":"Synthesis, Characterization, and Antibacterial Activity of N-substituted Quaternized Chitosan and Its Cellulose-based Composite Film","volume":"15","author":"Chen","year":"2020","journal-title":"BioResources"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"(2010). Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environ. Toxicol. Pharmacol., 30, 162\u2013168.","DOI":"10.1016\/j.etap.2010.05.004"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/2\/440\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:57:25Z","timestamp":1760173045000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/2\/440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,13]]},"references-count":46,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["polym12020440"],"URL":"https:\/\/doi.org\/10.3390\/polym12020440","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,13]]}}}