{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T23:32:59Z","timestamp":1773185579773,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T00:00:00Z","timestamp":1590364800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Zinc compounds in polyolefin films regulate the transmission of UV-VIS radiation, affect mechanical properties and antimicrobial activity. According to hypothesis, the use of zinc- containing masterbatches in polyethylene films (PE) with different chemical nature\u2014hydrophilic zinc oxide (ZO) and hydrophobic zinc stearate (ZS)\u2014can cause a synergistic effect, especially due to their antimicrobial properties. PE films obtained on an industrial scale containing zinc oxide and zinc stearate masterbatches were evaluated for antimicrobial activity against E. coli and S. aureus strains. The morphology of the samples (SEM), composition (EDX), UV barrier and transparency, mechanical properties and global migration level were also determined. SEM micrographs confirmed the good dispersion of zinc additives in the PE matrix. The use of both masterbatches in one material caused a synergistic effect of antimicrobial activity against both bacterial strains. The ZO masterbatch reduced the transparency of films, increased their UV-barrier ability and improved tensile strength, while the ZS masterbatch did not significantly change the tested parameters. The global migration limit was not exceeded for any of the samples. The use of ZO and ZS masterbatch mixtures enables the design of packaging with high microbiological protection with a controlled transmission for UV and VIS radiation.<\/jats:p>","DOI":"10.3390\/polym12051198","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T11:42:02Z","timestamp":1590406922000},"page":"1198","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The Synergistic Microbiological Effects of Industrial Produced Packaging Polyethylene Films Incorporated with Zinc Nanoparticles"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2748-0393","authenticated-orcid":false,"given":"Szymon","family":"Mania","sequence":"first","affiliation":[{"name":"Department of Chemistry, Technology, and Biotechnology of Food, Chemical Faculty, Gdansk University of Technology, 11\/12 G. Narutowicza Str., 80-233 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mateusz","family":"Cie\u015blik","sequence":"additional","affiliation":[{"name":"Department of Electrochemistry, Corrosion and Material Engineering, Chemical Faculty, Gdansk University of Technology, 11\/12 G. Narutowicza Str., 80-233 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Konzorski","sequence":"additional","affiliation":[{"name":"NAN company Andrzej Nelson Ma\u0142gorzata Nelson Sp. J., 35D Zaj\u0105czkowo Str., 83-111 Zaj\u0105czkowo, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawe\u0142","family":"\u015awi\u0119cikowski","sequence":"additional","affiliation":[{"name":"NAN company Andrzej Nelson Ma\u0142gorzata Nelson Sp. J., 35D Zaj\u0105czkowo Str., 83-111 Zaj\u0105czkowo, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Nelson","sequence":"additional","affiliation":[{"name":"NAN company Andrzej Nelson Ma\u0142gorzata Nelson Sp. J., 35D Zaj\u0105czkowo Str., 83-111 Zaj\u0105czkowo, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrianna","family":"Banach","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Technology, and Biotechnology of Food, Chemical Faculty, Gdansk University of Technology, 11\/12 G. Narutowicza Str., 80-233 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Tylingo","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Technology, and Biotechnology of Food, Chemical Faculty, Gdansk University of Technology, 11\/12 G. Narutowicza Str., 80-233 Gdansk, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"ref_1","unstructured":"Smithers Pira (2020, April 17). The Future of Global Plastic Films to 2021. Available online: https:\/\/www.smithers.com\/services\/market-reports\/materials\/the-future-of-global-plastic-films-to-2021."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1365-2621.2004.tb13375.x","article-title":"Antimicrobial Activity of Citric, Lactic, Malic or Tartaric Acids and Nisin-incorporated Soy Protein Film Against Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella gaminara","volume":"69","author":"Eswaranandam","year":"2004","journal-title":"J. Food Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"833","DOI":"10.4315\/0362-028X-67.4.833","article-title":"Antimicrobial Edible Films and Coatings","volume":"67","author":"Cagri","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1016\/j.ultsonch.2011.11.011","article-title":"Synthesis and characterization of Ag nanoparticles@polyethylene fibers under ultrasound irradiation","volume":"19","author":"Abbasi","year":"2002","journal-title":"Ultrason. Sonochem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.jcis.2004.02.012","article-title":"Silver nanoparticles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria","volume":"275","author":"Sondi","year":"2004","journal-title":"J. Colloid Interface Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1186\/s40824-016-0062-6","article-title":"Antibacterial potency of different deposition methods of silver and copper containing diamond-like carbon coated polyethylene","volume":"20","author":"Harrasser","year":"2016","journal-title":"Biomater. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"035009","DOI":"10.1088\/1748-6041\/8\/3\/035009","article-title":"In vivo evaluation of copper release and acute local tissue reactions after implantation of copper-coated titanium implants in rats","volume":"8","author":"Hoene","year":"2013","journal-title":"Biomed. Mater."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.3389\/fmicb.2018.01218","article-title":"Mechanism of Anti-bacterial Activity of Zinc Oxide Nanoparticle against Carbapenem-Resistant Acinetobacter baumannii","volume":"9","author":"Tiwari","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_9","unstructured":"(2020, April 17). FDA (2011) Part 182\u2014Substances Generally Recognized as Safe, Available online: https:\/\/www.accessdata.fda.gov\/scripts\/cdrh\/cfdocs\/cfcfr\/CFRSearch.cfm?CFRPart=182&showFR=1."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1007\/s11947-012-0797-6","article-title":"Zinc Oxide Nanoparticles: Synthesis, Antimicrobial Activity and Food Packaging Applications","volume":"5","author":"Espitia","year":"2012","journal-title":"Food Bioprocess. Technol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1111\/j.1574-6968.2007.01012.x","article-title":"Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms","volume":"279","author":"Jones","year":"2008","journal-title":"Fems Microbiol. Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1080\/02652030701744538","article-title":"Applications and implications of nanotechnologies for the food sector","volume":"25","author":"Chaudhry","year":"2008","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"100301","DOI":"10.1016\/j.fpsl.2019.100301","article-title":"Zinc migration and its effect on the functionality of a low-density polyethylene-ZnO nanocomposite film","volume":"20","author":"Bumbudsanpharoke","year":"2019","journal-title":"Food Packag. Shelf."},{"key":"ref_14","unstructured":"(2011). Commission Regulation (EU) No 10\/2011 of 14 January 2011 on plastic materials and articles intended to come into contact with food. Off. J. Eur. Union, L12, 1\u201389."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/8756087918822677","article-title":"Long- and short-term antibacterial properties of low-density polyethylene-based films coated with zinc oxide nanoparticles for potential use in food packaging","volume":"35","author":"Rokbani","year":"2019","journal-title":"J. Plast Film Sheet."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Al-Naamani, L., Dutta, J., and Dobretsov, S. (2018). Nanocomposite Zinc Oxide-Chitosan Coatings on Polyethylene Films for Extending Storage Life of Okra (Abelmoschus esculentus). Nanomaterials, 8.","DOI":"10.3390\/nano8070479"},{"key":"ref_17","first-page":"125915","article-title":"Antimicrobial food packaging based on sustainable Bio-based materials for reducing foodborne Pathogens: A review","volume":"310","author":"Youssef","year":"2019","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"055301","DOI":"10.1088\/2053-1591\/aaff8b","article-title":"Dynamic mechanical properties of zinc oxide reinforced linear low-density polyethylene composites","volume":"6","author":"Tharayil","year":"2019","journal-title":"Mater. Res. Express"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.compositesb.2019.05.054","article-title":"Effective antimicrobial materials based on low-density polyethylene (LDPE) with zinc oxide (ZnO) nanoparticles","volume":"172","author":"Rojas","year":"2019","journal-title":"Compos. Part B Eng."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.polymertesting.2018.11.018","article-title":"A high-density polyethylene container based on ZnO\/lignin dual fillers with potential antimicrobial activity","volume":"73","author":"Klapiszewski","year":"2019","journal-title":"Polym. Test."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"030041","DOI":"10.1063\/1.5021234","article-title":"UV-screening, transparency and water barrier properties of semi refined iota carrageenan packaging film incorporated with ZnO nanoparticles","volume":"1927","author":"Khoirunnisa","year":"2018","journal-title":"AIP Conf. Proc."},{"key":"ref_22","unstructured":"(2005). PN EN 118-1:2005 Materials and Articles in Contact with Foodstuffs-Plastics-Part 1: Guide to the Selection of Conditions and Test Methods for Overall Migration, Polish Committee for Standardization."},{"key":"ref_23","unstructured":"(2005). PN EN 118-3:2005 Materials and Articles in Contact with Foodstuffs-Plastics-Part 3: Test Methods for Overall Migration into Aqueous Food Simulants by Total Immersion, Polish Committee for Standardization."},{"key":"ref_24","unstructured":"(2005). PN EN 118-14:2005 Materials and Articles in Contact with Foodstuffs-Plastics-Part 14: Test Methods for \u2018Substitute Tests\u2019 for Overall Migration from Plastic Intended to Come into Contact with Fatty Foodstuffs Using Test Media Iso-octane and 95% Ethanol, Polish Committee for Standardization."},{"key":"ref_25","unstructured":"(2019). ISO 527-1:2019 Plastics-Determination of Tensile Properties-Part 1: General Principles, International Organization for Standardization."},{"key":"ref_26","unstructured":"(2018). ISO 527-3:2018 Plastics-Determination of tensile properties-Part 3: Test Conditions for Films and Sheets, International Organization for Standardization."},{"key":"ref_27","unstructured":"(2007). ISO 22196:2007 Measurement of Antimicrobial Activity on Plastics and Other Non-Porous Surfaces, International Organization for Standardization."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.fbp.2016.10.007","article-title":"The impact of zinc oxide particle morphology as an antimicrobial and when incorporated in poly(3-hydroxybutyrate-co-3-hydroxyvalerate) films for food packaging and food contact surfaces applications","volume":"101","author":"Fabra","year":"2017","journal-title":"Food Bioprod. Process."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1002\/pen.20279","article-title":"Properties of mLLDPE\/LDPE blends in film blowing","volume":"45","author":"Fang","year":"2005","journal-title":"Polym. Eng. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.foodhyd.2012.05.001","article-title":"Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese","volume":"30","author":"Ramos","year":"2013","journal-title":"Food Hydrocolloid."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11051-005-5131-z","article-title":"Effect of Particle Size on the Photocatalytic Activity of Nanoparticulate Zinc Oxide","volume":"8","author":"Dodd","year":"2006","journal-title":"J. Nanopart. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1016\/j.biortech.2008.12.045","article-title":"Preparation and characterization of glycerol plasticized-pea starch\/ZnO\u2013carboxymethylcellulose sodium nanocomposites","volume":"100","author":"Yu","year":"2009","journal-title":"Bioresour. Technol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/j.porgcoat.2012.01.007","article-title":"Preparation and characterization of UV-cured polyurethane acrylate\/ZnO nanocomposite films based on surface modified ZnO","volume":"74","author":"Kim","year":"2012","journal-title":"Prog. Org."},{"key":"ref_34","unstructured":"FDA (2014). Guidance for Industry, Assessing the Effects of Significant Manufacturing Process Changes, Including Emerging Technologies, on the Safety and Regulatory Status of Food Ingredients and Food Contact Substances, Including Food Ingredients that are Color Additives, Office of food additive safety, HFS-205 center for food safety and applied nutrition Food and Drug Administration. Available online: https:\/\/www.fda.gov\/regulatory-information\/search-fda-guidance-documents\/guidance-industry-assessing-effects-significant-manufacturing-process-changes-including-emerging."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jfda.2018.12.002","article-title":"The current application of nanotechnology in food and agriculture","volume":"27","author":"He","year":"2018","journal-title":"J. Food Drug Anal."},{"key":"ref_36","unstructured":"EC (2016). Commission Regulation (EU) 2016\/1416 of 24 August 2016 amending and correcting Regulation (EU) No 10\/2011 on plastic materials and articles intended to come into contact with food (Text with EEA relevance). Off. J. Eur. Union, L230, 22\u201342."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2814","DOI":"10.1002\/etc.340","article-title":"Zinc oxide-engineered nanoparticles: Dissolution and toxicity to marine phytoplankton","volume":"29","author":"Miao","year":"2010","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/toxsci\/kfs006","article-title":"Zeta potential and solubility to toxic ions as mechanisms of lung inflammation caused by metal\/metal oxide nanoparticles","volume":"126","author":"Cho","year":"2012","journal-title":"Toxicol Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1490","DOI":"10.1080\/19440049.2016.1217067","article-title":"Contents of Ag and other metals in food-contact plastics with nanosilver or Ag ion and their migration into food simulants","volume":"33","author":"Ozaki","year":"2016","journal-title":"Food Addit. Contam. A"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1080\/19440049.2013.878812","article-title":"Study of silver ion migration from melt-blended and layered-deposited silver polyethylene nanocomposite into food simulants and apple juice","volume":"31","author":"Jokar","year":"2014","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"488","DOI":"10.17113\/ftb.53.04.15.3817","article-title":"Preparation and Application of LDPE\/ZnO Nanocomposites for Extending Shelf Life of Fresh Strawberries","volume":"53","author":"Emamifar","year":"2015","journal-title":"Food Technol. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Cirillo, G., Kozlowski, M.A., and Spizzirri, U.G. (2018). Zinc Composite Materials and Food Packaging. Composites Materials for Food Packaging, Scrivener Publishing LLC. [1st ed.].","DOI":"10.1002\/9781119160243"},{"key":"ref_43","first-page":"61","article-title":"Edible and Biodegradable Polymer Films: Challenges and Opportunities","volume":"51","author":"Krochta","year":"1997","journal-title":"Food Technol."},{"key":"ref_44","first-page":"37","article-title":"Method for Testing the Antimicrobial Character of the Materials and Their Fitting to the Scope","volume":"7","author":"Damian","year":"2014","journal-title":"Bull. Transylv. Univ. Bras"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"105101","DOI":"10.1088\/0957-4484\/22\/10\/105101","article-title":"Antifungal activity of ZnO nanoparticles- the role of ROS mediated cell injury","volume":"22","author":"Lipovsky","year":"2011","journal-title":"Nanotechnology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1016\/j.envpol.2008.12.025","article-title":"Bacterial toxicity comparison between nano- and microscaled oxide particles","volume":"157","author":"Jiang","year":"2009","journal-title":"Environ. Pollut."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.colsurfa.2014.05.057","article-title":"The contribution of zinc ions of the antimicrobial activity of zinc oxide","volume":"457","author":"Pasquet","year":"2014","journal-title":"Colloids Surf. Physicochem. Eng. Asp."},{"key":"ref_48","first-page":"1368","article-title":"Antibacterial activity of ZnO nanoparticle on Gram-positive and Gram-negative bacteria","volume":"5","author":"Karvani","year":"2012","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_49","first-page":"869","article-title":"Antimicrobial modification of polypropylene with silver nanoparticles immobilized on zinc stearate","volume":"50","author":"Holcapkova","year":"2016","journal-title":"Mater Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1007\/s10118-018-2120-0","article-title":"Effect of a Hybrid Zinc Stearate-Silver System on the Properties of Polylactide and Its Abiotic and the Biotic Degradation and Antimicrobial Activity Thereof","volume":"36","author":"Stoplova","year":"2018","journal-title":"Chin. J. Polym. Sci."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0378-1119(96)00323-X","article-title":"Bacterial resistances to toxic metal ions\u2014a review","volume":"179","author":"Silver","year":"1996","journal-title":"Gene"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.mito.2004.11.001","article-title":"Zinc causes loss of membrane potential and elevates reactive oxygen species in rat brain mitochondria","volume":"5","author":"Dineley","year":"2005","journal-title":"Mitochondrion"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ijmm.2014.11.005","article-title":"Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia Coli","volume":"305","author":"Salem","year":"2015","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1046\/j.1471-4159.2003.01678.x","article-title":"Zinc inhibition of cellular energy production: Implications for mitochondria and neurodegeneration","volume":"85","author":"Dineley","year":"2003","journal-title":"J. Neurochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s12951-018-0336-3","article-title":"Synergistic mechanism of Ag+\u2013Zn2+ in anti-bacterial activity against Enterococcus faecalis and its application against dentin infection","volume":"16","author":"Fan","year":"2018","journal-title":"J. Nanobiotechnol."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/5\/1198\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T09:32:06Z","timestamp":1760175126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/12\/5\/1198"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,25]]},"references-count":55,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,5]]}},"alternative-id":["polym12051198"],"URL":"https:\/\/doi.org\/10.3390\/polym12051198","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,25]]}}}