{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:47:06Z","timestamp":1790232426992,"version":"4.1.0"},"reference-count":60,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,19]],"date-time":"2020-07-19T00:00:00Z","timestamp":1595116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["15H02121"],"award-info":[{"award-number":["15H02121"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>We measured the changes in the higher-order structure of DNA molecules (\u03bb phage DNA, 48 kbp) at different concentrations of 1- and 2-propanol through single-molecular observation. It is known that 2-propanol is usually adapted for the procedure to isolate genomic DNA from living cells\/organs in contrast to 1-propanol. In the present study, it was found that with an increasing concentration of 1-propanol, DNA exhibits reentrant conformational transitions from an elongated coil to a folded globule, and then to an unfolded state. On the other hand, with 2-propanol, DNA exhibits monotonous shrinkage into a compact state. Stretching experiments under direct current (DC) electrical potential revealed that single DNA molecules intermediately shrunk by 1- and 2-propanol exhibit intrachain phase segregation, i.e., coexistence of elongated and compact parts. The characteristic effect of 1-propanol causing the reentrant transition is argued in terms of the generation of water-rich nanoclusters.<\/jats:p>","DOI":"10.3390\/polym12071607","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T05:10:30Z","timestamp":1595394630000},"page":"1607","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Marked Difference in the Conformational Transition of DNA Caused by Propanol Isomer"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8924-2860","authenticated-orcid":false,"given":"Yue","family":"Ma","sequence":"first","affiliation":[{"name":"Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe 610-0394, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe 610-0394, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidehiro","family":"Oana","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, The University of Tokyo, Tokyo 113-8656, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2751-7136","authenticated-orcid":false,"given":"Kenichi","family":"Yoshikawa","sequence":"additional","affiliation":[{"name":"Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe 610-0394, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,19]]},"reference":[{"key":"ref_1","first-page":"3","article-title":"Ethanol precipitation: Ammonium acetate as an alternative to sodium acetate","volume":"9","author":"Crouse","year":"1987","journal-title":"Focus"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1093\/nar\/18.2.378","article-title":"Ethanol precipitation of DNA with linear polyacrylamide as carrier","volume":"18","author":"Gaillard","year":"1990","journal-title":"Nucleic Acids Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1089\/hum.1995.6.5-565","article-title":"Cancer gene therapy using plasmid DNA: Purification of DNA for human clinical trials","volume":"6","author":"Horn","year":"1995","journal-title":"Hum. 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