{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:46:40Z","timestamp":1790999200506,"version":"4.1.0"},"reference-count":46,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T00:00:00Z","timestamp":1621987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kasetsart University Research and Development Institute  (KURDI), Kasetsart University","award":["\u0e1b-3.1(\u0e14)27.61"],"award-info":[{"award-number":["\u0e1b-3.1(\u0e14)27.61"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>This paper is aimed at investigating the usage of biosynthesized poly(3-hydroxybutyrate) (P(3-HB)) for a coating on pineapple leaf fiber paper (PLFP). For this purpose, (P(3-HB)) was produced by Rhodococcus pyridinivorans BSRT1-1, a highly potential P(3-HB) producing bacterium, with a weight-average molecular weight (Mw) of 6.07 \u00d7 10 \u22125 g\/mol. This biosynthesized P(3-HB) at 7.5% (w\/v) was then coated on PLFP through the dip-coating technique with chloroform used as a solvent. The respective coated PLFP showed that P(3-HB) could be well coated all over on the PLFP surface as confirmed by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The brightness and mechanical properties of PLFP could be improved by coating with biosynthesized P(3-HB) in comparison to commercially available P(3-HB) and non-coated PLFP. Furthermore, coating of P(3-HB) significantly increased the water drop penetration time on the surface of PLFP and was similar to that of the commercial P(3-HB) with the same content. The results showed that all the coated PLPF samples can be degraded under the soil burial test conditions. We have demonstrated that the P(3-HB) coated PLFP paper has the ability to prevent water drop penetration and could undergo biodegradation. Taken together, the P(3-HB) coated PLFP can be applied as a promising biodegradable paper packaging.<\/jats:p>","DOI":"10.3390\/polym13111733","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T21:56:44Z","timestamp":1622066204000},"page":"1733","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biosynthesized Poly(3-Hydroxybutyrate) on Coated Pineapple Leaf Fiber Papers for Biodegradable Packaging Application"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4280-2426","authenticated-orcid":false,"given":"Pilanee","family":"Vaithanomsat","sequence":"first","affiliation":[{"name":"Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand"},{"name":"Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunat","family":"Kongsin","sequence":"additional","affiliation":[{"name":"Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand"},{"name":"Biotechnology of Biopolymers and Bioactive Compounds Special Research Unit, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0174-7651","authenticated-orcid":false,"given":"Chanaporn","family":"Trakunjae","sequence":"additional","affiliation":[{"name":"Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand"},{"name":"Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirachaya","family":"Boonyarit","sequence":"additional","affiliation":[{"name":"Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amnat","family":"Jarerat","sequence":"additional","affiliation":[{"name":"Food Technology Program, Kanchanaburi Campus, Mahidol University, Kanchanaburi 71150, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4756-9192","authenticated-orcid":false,"given":"Kumar","family":"Sudesh","sequence":"additional","affiliation":[{"name":"Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9659-7854","authenticated-orcid":false,"given":"Rungsima","family":"Chollakup","sequence":"additional","affiliation":[{"name":"Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1080\/15440478.2014.984060","article-title":"Pulping process and the potential of using non-wood pineapple leaves fiber for pulp and paper production: A review","volume":"13","author":"Laftah","year":"2015","journal-title":"J. Nat. Fibers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"106927","DOI":"10.1016\/j.compositesb.2019.106927","article-title":"Review on mechanical properties evaluation of pineapple leaf fibre (PALF) reinforced polymer composites","volume":"174","author":"Todkar","year":"2019","journal-title":"Compos. B Eng."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1002\/app.32910","article-title":"Pineapple leaf fiber reinforced thermoplastic composites: Effects of fiber length and fiber content on their characteristics","volume":"119","author":"Chollakup","year":"2011","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1177\/0892705713489701","article-title":"Effect of pineapple leaf fiber-reinforced thermoplastic starch\/poly(lactic acid) green composite","volume":"28","author":"Smitthipong","year":"2013","journal-title":"J. Thermoplast. Compos. Mater."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1177\/0892705716632860","article-title":"Modification of pineapple leaf fiber surfaces with silane and isocyanate for reinforcing thermoplastic","volume":"30","author":"Suwanruji","year":"2016","journal-title":"J. Thermoplast. Compos. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1177\/0892705715598356","article-title":"Initial properties and ageing behaviour of pineapple leaf and palm fibre as reinforcement for polypropylene","volume":"30","author":"Chollakup","year":"2016","journal-title":"J. Thermoplast. Compos. Mater."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"5978","DOI":"10.1016\/j.jece.2017.11.026","article-title":"Production of paper from pineapple leaves","volume":"5","author":"Sibaly","year":"2017","journal-title":"J. Environ. Chem. Eng."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Simona, J., Dani, D., Petr, S., Marcela, N., Jakub, T., and Bohuslava, T. (2021). Edible films from carrageenan\/orange essential oil\/trehalose\u2014structure, optical properties, and antimicrobial activity. Polymers, 13.","DOI":"10.3390\/polym13030332"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1541-4337.2009.00095.x","article-title":"Biopolymer coatings on paper packaging materials","volume":"9","author":"Khwaldia","year":"2010","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"887","DOI":"10.3390\/coatings5040887","article-title":"Bio-based coatings for paper applications","volume":"5","author":"Rastogi","year":"2015","journal-title":"Coatings"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1111\/1751-7915.12129","article-title":"Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): A review of recent research work","volume":"7","author":"Pena","year":"2014","journal-title":"Microb. Biotechnol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"124737","DOI":"10.1016\/j.biortech.2021.124737","article-title":"Polyhydroxyalkanoates: Trends and advances toward biotechnological applications","volume":"326","author":"Kalia","year":"2021","journal-title":"Bioresour. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ragaert, P., Buntinx, M., Maes, C., Vanheusden, C., Peeters, R., Wang, S., D\u2019Hooge, D.R., and Cardon, L. (2019). Polyhydroxyalkanoates for Food Packaging Applications. Reference Module in Food Science, Elsevier.","DOI":"10.1016\/B978-0-08-100596-5.22502-X"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.colsurfa.2012.09.052","article-title":"Modification of paper using polyhydroxybutyrate to obtain biomimetic superhydrophobic substrates","volume":"416","author":"Obeso","year":"2013","journal-title":"Colloids Surf. Physicochem. Eng. Aspects"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"6274","DOI":"10.1016\/j.polymer.2009.10.065","article-title":"Biocomposites based on renewable resource: Acetylated and non acetylated cellulose cardboard coated with polyhydroxybutyrate","volume":"50","author":"Cyras","year":"2009","journal-title":"Polymer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1002\/app.26663","article-title":"Biodegradable double-layer films based on biological resources: Polyhydroxybutyrate and cellulose","volume":"106","author":"Cyras","year":"2007","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17884","DOI":"10.1038\/srep17884","article-title":"Tuning the properties of polyhydroxybutyrate films using acetic acid via solvent casting","volume":"5","author":"Anbukarasu","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1023\/A:1010124205584","article-title":"PHB\/V in extrusion coating of paper and paperboard: Part I: Study of functional properties","volume":"8","author":"Kuusipalo","year":"2000","journal-title":"J. Polym. Environ."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2419","DOI":"10.1007\/s10570-018-1729-z","article-title":"Bilayer biocomposites based on coated cellulose paperboard with films of polyhydroxybutyrate\/cellulose nanocrystals","volume":"25","author":"Seoane","year":"2018","journal-title":"Cellulose"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1016\/j.mspro.2015.04.139","article-title":"Properties and processing relationship of polyhydroxybutyrate and cellulose biocomposites","volume":"8","author":"Seoane","year":"2015","journal-title":"Procedia Mater. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"46872","DOI":"10.1002\/app.46872","article-title":"Enhancement of paperboard performance as packaging material by layering with plasticized polyhydroxybutyrate\/nanocellulose coatings","volume":"135","author":"Seoane","year":"2018","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1038\/s41598-021-81386-2","article-title":"Enhanced polyhydroxybutyrate (PHB) production by newly isolated rare actinomycetes Rhodococcus sp. strain BSRT1-1 using response surface methodology","volume":"11","author":"Trakunjae","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.jtusci.2015.09.003","article-title":"Recovery and subsequent characterization of polyhydroxybutyrate from Rhodococcus equi cells grown on crude palm kernel oil","volume":"10","author":"Altaee","year":"2016","journal-title":"J. Taibah Univ. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"141","DOI":"10.4028\/www.scientific.net\/KEM.847.141","article-title":"Development of rice straw paper coated with pomelo peel extract for bio-based and antibacterial packaging","volume":"847","author":"Chollakup","year":"2020","journal-title":"Key Eng. Mater."},{"key":"ref_25","unstructured":"TAPPI (2021, January 19). Standards Brightness of Pulp, Paper, and Paperboard (Directional Reflectance at 457 nm), Test Method. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T452.aspx."},{"key":"ref_26","unstructured":"TAPPI (2021, January 19). Tensile Properties of Paper and Paperboard (Using Constant Rate of Elongation Apparatus), Test Method TAPPI\/ANSI 2009. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T494.aspx."},{"key":"ref_27","unstructured":"TAPPI (2021, January 19). Folding Endurance of Paper (Schopper Type Tester), Test Method 2007. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T423.aspx."},{"key":"ref_28","unstructured":"TAPPI (2021, January 19). Internal Tearing Resistance of Paper (Elmendorf-Type Method), Test Method. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T414.aspx."},{"key":"ref_29","unstructured":"TAPPI (2021, January 19). Bursting Strength of Paper, Test Method 2009. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T414.aspx."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Seoane, I.T., Manfredi, L.B., Cyras, V.P., Torre, L., Fortunati, E., and Puglia, D. (2017). Effect of cellulose nanocrystals and bacterial cellulose on disintegrability in composting conditions of plasticized PHB nanocomposites. Polymers, 9.","DOI":"10.3390\/polym9110561"},{"key":"ref_31","unstructured":"TAPPI (2021, January 19). Water Absorption of Corrugating Medium: Water Drop Absoption Test, Test Method TAPPI\/ANSI 2009. Available online: https:\/\/imisrise.tappi.org\/TAPPI\/Products\/01\/T\/0104T835.aspx."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.bej.2011.12.013","article-title":"On the control of molecular weight distribution of polyhydroxybutyrate in Azohydromonas lata cultures","volume":"62","author":"Penloglou","year":"2012","journal-title":"Biochem. Eng. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1002\/pen.760220402","article-title":"Influence of molecular weight and molecular weight distribution on mechanical properties of polymers","volume":"22","author":"Nunes","year":"1982","journal-title":"Polym. Eng. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"McAdam, B., Brennan Fournet, M., McDonald, P., and Mojicevic, M. (2020). Production of polyhydroxybutyrate (PHB) and factors impacting its chemical and mechanical characteristics. Polymers, 12.","DOI":"10.3390\/polym12122908"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1002\/elsc.200900034","article-title":"Recovery of polyhydroxybutyrate (PHB) from Cupriavidus necator biomass by solvent extraction with 1,2-propylene carbonate","volume":"9","author":"Fiorese","year":"2009","journal-title":"Eng. Life Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ijbiomac.2013.04.040","article-title":"A statistical approach for optimization of polyhydroxybutyrate production by marine Bacillus subtilis MSBN17","volume":"59","author":"Sathiyanarayanan","year":"2013","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.jclepro.2018.12.239","article-title":"Production of bioplastic (poly-3-hydroxybutyrate) using waste paper as a feedstock: Optimization of enzymatic hydrolysis and fermentation employing Burkholderia sacchari","volume":"214","author":"Annamalai","year":"2019","journal-title":"J. Clean. Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/j.biortech.2006.02.022","article-title":"Characterization of poly(3-hydroxybutyrate) produced by Cupriavidus necator in solid-state fermentation","volume":"98","author":"Oliveira","year":"2007","journal-title":"Bioresour. Technol."},{"key":"ref_39","unstructured":"Chaijamrus, S., and Udpuay, N. (2021, January 19). Production and Characterization of Polyhydroxybutyrate from Molasses and Corn Steep Liquor Produced by Bacillus megaterium ATCC 6748. Available online: https:\/\/cigrjournal.org\/index.php\/Ejounral\/article\/view\/1216."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.ijbiomac.2014.09.028","article-title":"Production of copolymer, poly (hydroxybutyrate-co-hydroxyvalerate) by Halomonas campisalis MCM B-1027 using agro-wastes","volume":"72","author":"Kulkarni","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3438","DOI":"10.15376\/biores.8.3.3438-3452","article-title":"Physical and mechanical properties of hydroxypropyl methylcellulose\u2013coated paper as affected by coating weight and coating composition","volume":"8","author":"Khwaldia","year":"2013","journal-title":"BioResources"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.foodres.2008.12.003","article-title":"Effect of hydroxypropyl methylcellulose and lipid on mechanical properties and water vapor permeability of coated paper","volume":"42","author":"Sothornvit","year":"2009","journal-title":"Food. Res. Inter."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1002\/cjce.21708","article-title":"Effect of particle size of magnesium silicate filler on physical properties of paper","volume":"91","author":"Chauhan","year":"2013","journal-title":"Can. J. Chem. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.polymdegradstab.2014.01.034","article-title":"Disintegrability under composting conditions of plasticized PLA\u2013PHB blends","volume":"108","author":"Arrieta","year":"2014","journal-title":"Polym. Degrad. Stab."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Siakeng, R., Jawaid, M., Asim, M., and Siengchin, S. (2020). Accelerated weathering and soil burial effect on biodegradability, colour and textureof coir\/pineapple leaf fibres\/PLA biocomposites. Polymers, 12.","DOI":"10.3390\/polym12020458"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Arrieta, M.P., Samper, M.D., Aldas, M., and L\u00f3pez, J. (2017). On the use of PLA-PHB blends for sustainable food packaging applications. Materials, 10.","DOI":"10.3390\/ma10091008"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/11\/1733\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:07:52Z","timestamp":1760162872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/11\/1733"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,26]]},"references-count":46,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["polym13111733"],"URL":"https:\/\/doi.org\/10.3390\/polym13111733","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,26]]}}}