{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T03:26:10Z","timestamp":1782444370673,"version":"3.54.5"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Faculty of Medical Technology, Prince of Songkla University and by Ernst Mach Grant (ASEA-UNINET)."}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Herein we demonstrate molecularly imprinted polymers (MIP) as plastic antibodies for a microplate-based assay. As the most abundant plasma protein, human serum albumin (HSA) was selected as the target analyte model. Thin film MIP was synthesized by the surface molecular imprinting approach using HSA as the template. The optimized polymer consisted of acrylic acid (AA) and N-vinylpyrrolidone (VP) in a 2:3 (w\/w) ratio, crosslinked with N,N\u2032-(1,2-dihydroxyethylene) bisacrylamide (DHEBA) and then coated on the microplate well. The binding of MIP toward the bound HSA was achieved via the Bradford reaction. The assay revealed a dynamic detection range toward HSA standards in the clinically relevant 1\u201310 g\/dL range, with a 0.01 g\/dL detection limit. HSA-MIP showed minimal interference from other serum protein components: \u03b3-globulin had 11% of the HSA response, \u03b1-globulin of high-density lipoprotein had 9%, and \u03b2-globulin of low-density lipoprotein had 7%. The analytical accuracy of the assay was 89\u2013106% at the 95% confidence interval, with precision at 4\u20139%. The MIP-coated microplate was stored for 2 months at room temperature without losing its binding ability. The results suggest that the thin film plastic antibody system can be successfully applied to analytical\/pseudoimmunological HSA determinations in clinical applications.<\/jats:p>","DOI":"10.3390\/polym13111763","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T11:07:02Z","timestamp":1622113622000},"page":"1763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Thin Film Plastic Antibody-Based Microplate Assay for Human Serum Albumin Determination"],"prefix":"10.3390","volume":"13","author":[{"given":"Worachote","family":"Boonsriwong","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Bangkok Thonburi University, Bangkok 10170, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5154-6439","authenticated-orcid":false,"given":"Suticha","family":"Chunta","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Faculty of Medical Technology, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nonthawat","family":"Thepsimanon","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Faculty of Medical Technology, Prince of Songkla University, Songkhla 90110, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanita","family":"Singsanan","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Faculty of Allied Health Sciences, Burapha University, Chonburi 20131, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1596-0584","authenticated-orcid":false,"given":"Peter A.","family":"Lieberzeit","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Faculty for Chemistry, University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0167-7799(00)01523-7","article-title":"Molecular imprinting: At the edge of the third millennium","volume":"19","author":"Piletsky","year":"2001","journal-title":"Trends Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/j.medengphy.2006.05.004","article-title":"Molecularly imprinted polymers in clinical diagnostics\u2014Future potential and existing problems","volume":"28","author":"Piletsky","year":"2006","journal-title":"Med. 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