{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:16:12Z","timestamp":1792034172649,"version":"4.4.0"},"reference-count":48,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,28]],"date-time":"2021-11-28T00:00:00Z","timestamp":1638057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>In this study, non-chlorinated solvents such as cyclohexanone (CYC) and three ionic liquids, (ILs) (1-ethyl-3-methylimidazolium dimethylphosphate, [EMIM][DMP], 1-ethyl-3-methylimidazolium diethylphosphate, [EMIM][DEP] and 1-ethyl-3-methylimidazolium methylphosphite, [EMIM][MP]) were tested to extract polyhydroxyalkanoates (PHAs) from the purple non-sulfur photosynthetic bacterium (PNSB) Rhodovulumsulfidophilum DSM-1374. The photosynthetic bacterium was cultured in a new generation photobioreactor with 4 L of working volume using a lactate-rich medium. The extracted PHAs were characterized using a thermogravimetric analysis, differential scanning calorimetry, infrared spectroscopy, proton nuclear magnetic resonance and gel permeation chromatography. The most promising results were obtained with CYC at 125 \u00b0C with an extraction time of above 10 min, obtaining extraction yields higher than 95% and a highly pure poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with around 2.7 mol% of hydroxylvalerate (HV). A similar yield and purity were obtained with chloroform (CHL) at 10 \u00b0C for 24 h, which was used as the referent solvent Although the three investigated ILs at 60 \u00b0C for 4 and 24 h with biomass\/IL up to 1\/30 (w\/w) obtained PHAs strongly contaminated by cellular membrane residues, they were not completely solubilized by the investigated ILs.<\/jats:p>","DOI":"10.3390\/polym13234163","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T02:34:05Z","timestamp":1638326045000},"page":"4163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Extraction of Polyhydroxyalkanoates from Purple Non-Sulfur Bacteria by Non-Chlorinated Solvents"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9772-7254","authenticated-orcid":false,"given":"Sara","family":"Filippi","sequence":"first","affiliation":[{"name":"Department of Civil and Industrial Engineering, University of Pisa, L.go Lucio Lazzarino 1, 56122 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2401-133X","authenticated-orcid":false,"given":"Patrizia","family":"Cinelli","sequence":"additional","affiliation":[{"name":"Department of Civil and Industrial Engineering, University of Pisa, L.go Lucio Lazzarino 1, 56122 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4540-9948","authenticated-orcid":false,"given":"Andrea","family":"Mezzetta","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, Via Bonanno, 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6197-5563","authenticated-orcid":false,"given":"Pietro","family":"Carlozzi","sequence":"additional","affiliation":[{"name":"Research Institute on Terrestrial Ecosystems, CNR, Via Madonna del Piano 10, 50019 Florence, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9628-7178","authenticated-orcid":false,"given":"Maurizia","family":"Seggiani","sequence":"additional","affiliation":[{"name":"Department of Civil and Industrial Engineering, University of Pisa, L.go Lucio Lazzarino 1, 56122 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1016\/S0079-6700(00)00035-6","article-title":"Synthesis, Structure and Properties of Polyhydroxyalkanoates: Biological Polyesters","volume":"25","author":"Sudesh","year":"2000","journal-title":"Prog. 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