{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T14:34:12Z","timestamp":1787927652814,"version":"build-2784847793"},"reference-count":49,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T00:00:00Z","timestamp":1638230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>A synbiotic comprising Saccharomyces cerevisiae yeast (SCY) and Moringa oleifera leaf extract (MOLE) has been encapsulated using nanotechnology. This duo is used as a dietary supplement for growing rabbits. Physicochemical analyses, in vitro antimicrobial activity, and gastrointestinal system evaluation were used to evaluate the quality of the nanofabricated synbiotic. The in vivo study was conducted using 40-day-old male growing rabbits (n = 16 rabbits\/group) to evaluate the effect of the nanofabricated synbiotic on the health and growth performance of examined rabbits. Rabbits were equally allocated into four groups; (a) NCS, which received a basal diet supplemented with a noncapsulated 11 \u00d7 1012 CFU SCY + 0.15 g MOLE\/kg diet, (b) LCS: those receiving a nanoencapsulated 5.5 \u00d7 1012 CFU SCY + 0.075 g MOLE\/kg diet, (c) HCS: those receiving an 11 \u00d7 1012 CFU SCY + 0.15 g MOLE\/kg diet, and (d) CON: those receiving a basal diet without treatment (control). The treatments continued from day 40 to day 89 of age. During the experimental period, growth performance variables, including body weight (BW), feed consumption, BW gain, and feed conversion ratio were recorded weekly. Blood samples were collected on day 40 of age and immediately before the start of the treatments to confirm the homogeneity of rabbits among groups. On day 89 of age, blood samples, intestinal, and cecal samples were individually collected from eight randomly selected rabbits. The size and polydispersity index of the nanofabricated synbiotic were 51.38 nm and 0.177, respectively. Results revealed that the encapsulation process significantly improved yeast survival through the gastrointestinal tract, specifically in stomach acidic conditions, and significantly increased in vitro inhibitory activities against tested pathogens. Furthermore, treatments had no negative effects on hematobiochemical variables but significantly improved levels of blood plasma, total protein, and insulin-like growth factor-l. Compared to the CON, NCS, and LCS treatments, the HCS treatment increased the amount of intestinal and cecal yeast cells (p &lt; 0.05) and Lactobacillus bacteria (p &lt; 0.05) and decreased number of Salmonella (p &lt; 0.05) and Coliform (p = 0.08) bacteria. Likewise, both LCS and HCS significantly improved the small intestine and cecum lengths compared to CON and NCS. The HCS treatment also significantly improved BW gain and feed conversion compared to CON treatment, whereas the NCS and LCS treatments showed intermediate values. Conclusively, the nanoencapsulation process improved the biological efficiency of the innovative synbiotic used in this study. A high dose of encapsulated synbiotic balanced the gut microflora, resulting in the growth of rabbits during the fattening period.<\/jats:p>","DOI":"10.3390\/polym13234191","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T02:34:05Z","timestamp":1638326045000},"page":"4191","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effect of Nanoencapsulated Alginate-Synbiotic on Gut Microflora Balance, Immunity, and Growth Performance of Growing Rabbits"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0058-9671","authenticated-orcid":false,"given":"Nesrein M.","family":"Hashem","sequence":"first","affiliation":[{"name":"Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourhan S.","family":"Hosny","sequence":"additional","affiliation":[{"name":"Department of Livestock Research, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Alexandria 21934, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagwa I.","family":"El-Desoky","sequence":"additional","affiliation":[{"name":"Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, Alexandria 21545, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed G.","family":"Shehata","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El Arab, Alexandria 21934, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.aoas.2017.11.003","article-title":"Inclusion of phytogenic feed additives comparable to vitamin E in diet of growing rabbits: Effects on metabolism and growth","volume":"62","author":"Hashem","year":"2017","journal-title":"Ann. Agric. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.molmed.2016.04.003","article-title":"Antibiotic-Induced changes in the intestinal microbiota and disease","volume":"22","author":"Becattini","year":"2016","journal-title":"Trends Mol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.livsci.2013.06.017","article-title":"Growth performance, apparent nutrient digestibility, caecal fermentation, ileal morphology and caecal microflora of growing rabbits fed diets containing probiotics and prebiotics","volume":"157","author":"Oso","year":"2013","journal-title":"Livest. Sci."},{"key":"ref_4","first-page":"1","article-title":"Effect of Dietary Supplementation with Probiotics and Prebiotics on Haematological Indices, Serum Chemistry and Gut Salmonella Count of Broilers Sourced from Salmonella-infected Hatcheries in South-west Zone of Nigeria","volume":"10","author":"Olorunsola","year":"2016","journal-title":"Am. J. Exp. Agric."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"47856","DOI":"10.1007\/s11356-021-15460-7","article-title":"The use of some plant-derived products as effective alternatives to antibiotic growth promoters in organic poultry production: A review","volume":"28","author":"Seidavi","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Chen, H.-J., Yang, W.-Y., and Wang, C.-Y. (2017, January 17\u201319). The review on the function of intestinal flora and the regulatory effects of probiotics on the intestinal health of rabbits. Proceedings of the 2017 2nd international conference on biological sciences and technology (BST 2017), Zhuhai, China.","DOI":"10.2991\/bst-17.2018.8"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2154","DOI":"10.3168\/jds.S0022-0302(05)72891-5","article-title":"Health and Growth of Veal Calves Fed Milk Replacers With or Without Probiotics","volume":"88","author":"Timmerman","year":"2005","journal-title":"J. Dairy Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.livsci.2019.08.012","article-title":"Effects of Moringa oleifera extracts and monensin on performance of growing rabbits","volume":"228","author":"Hashem","year":"2019","journal-title":"Livest. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.anifeedsci.2018.01.004","article-title":"Progressive adaptation of sheep to a microencapsulated blend of essential oils: Ruminal fermentation, methane emission, nutrient digestibility, and microbial protein synthesis","volume":"237","author":"Soltan","year":"2018","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13099-018-0250-0","article-title":"The role of probiotics, prebiotics and synbiotics in animal nutrition","volume":"10","author":"Markowiak","year":"2018","journal-title":"Gut Pathog."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetimm.2018.12.006","article-title":"Effects and immune responses of probiotic treatment in ruminants","volume":"208","author":"Raabis","year":"2019","journal-title":"Veter. Immunol. Immunopathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1549","DOI":"10.1017\/S1751731117000088","article-title":"Physiological response and semen quality of rabbit bucks supplemented with Moringa leaves ethanolic extract during summer season","volume":"11","author":"Hashem","year":"2017","journal-title":"Animal"},{"key":"ref_13","first-page":"86","article-title":"Effect of Moringa oleifera leaves extract as a growth factor on viability of some encapsulated probiotic bacteria","volume":"9","author":"Amer","year":"2014","journal-title":"World J. Dairy Food Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Staniszewski, A., and Kordowska-Wiater, M. (2021). Probiotic and Potentially Probiotic Yeasts\u2014Characteristics and Food Application. Foods, 10.","DOI":"10.3390\/foods10061306"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1016\/j.automatica.2015.02.002","article-title":"Containment control of heterogeneous linear multi-agent systems","volume":"54","author":"Haghshenas","year":"2015","journal-title":"Automatica"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1134\/S0026893312030077","article-title":"The combined application of nucleotide and amino acid sequences of NS3 hepatitis C virus protein, DNA encoding granulocyte macrophage colony-stimulating factor, and inhibitor of regulatory T cells induces effective immune responce against Hepatitis C virus","volume":"46","author":"Masalova","year":"2012","journal-title":"Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"El-Desoky, N.I., Hashem, N.M., Gonzalez-Bulnes, A., Elkomy, A.G., and Abo-Elezz, Z.R. (2021). Effects of a Nanoencapsulated Moringa Leaf Ethanolic Extract on the Physiology, Metabolism and Reproductive Performance of Rabbit Does during Summer. Antioxidants, 10.","DOI":"10.3390\/antiox10081326"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Hassanein, E., Hashem, N., El-Azrak, K., Gonzalez-Bulnes, A., Hassan, G., and Salem, M. (2021). Efficiency of GnRH\u2013Loaded Chitosan Nanoparticles for Inducing LH Secretion and Fertile Ovulations in Protocols for Artificial Insemination in Rabbit Does. Animals, 11.","DOI":"10.3390\/ani11020440"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.3923\/ijds.2017.81.92","article-title":"Improving the Nutritional Value and Extending Shelf Life of Labneh by Adding Moringa oleifera Oil","volume":"12","author":"Salama","year":"2017","journal-title":"Int. J. Dairy Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food\u2013An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.5713\/ajas.2009.80298","article-title":"Effect of Butyric Acid on Performance, Gastrointestinal Tract Health and Carcass Characteristics in Broiler Chickens","volume":"22","author":"Panda","year":"2009","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_22","unstructured":"NRC (1977). Nutrient Requirements of Rabbits, National Academy of Sciences."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1287\/orsc.11.6.611.12529","article-title":"Organizational Routines as a Source of Continuous Change","volume":"11","author":"Feldman","year":"2000","journal-title":"Organ. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hashem, N.M., and Shehata, M.G. (2021). Antioxidant and Antimicrobial Activity of Cleomedroserifolia (Forssk.) Del. and Its Biological Effects on Redox Status, Immunity, and Gut Microflora. Animals, 11.","DOI":"10.3390\/ani11071929"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Cotozzolo, E., Cremonesi, P., Curone, G., Menchetti, L., Riva, F., Biscarini, F., Marongiu, M.L., Castrica, M., Castiglioni, B., and Miraglia, D. (2020). Characterization of Bacterial Microbiota Composition along the Gastrointestinal Tract in Rabbits. Animals, 11.","DOI":"10.3390\/ani11010031"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"198","DOI":"10.5713\/ajas.15.0823","article-title":"Effects of probiotic supplement (Bacillus subtilis and Lactobacillus acidophilus) on feed efficiency, growth performance, and microbial population of weaning rabbits","volume":"30","author":"Phuoc","year":"2016","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.anifeedsci.2017.11.010","article-title":"Yeast and yeast derivatives in feed additives and ingredients: Sources, characteristics, animal responses, and quantification methods","volume":"235","author":"Shurson","year":"2018","journal-title":"Anim. Feed. Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1038\/ajg.2012.56","article-title":"Saccharomyces boulardiifor the Prevention of Antibiotic-Associated Diarrhea in Adult Hospitalized Patients: A Single-Center, Randomized, Double-Blind, Placebo-Controlled Trial","volume":"107","author":"Pozzoni","year":"2012","journal-title":"Off. J. Am. Coll. Gastroenterol. |ACG"},{"key":"ref_29","first-page":"3","article-title":"Prebiotics and probiotics: An assessment of their safety and health benefits","volume":"1","year":"2016","journal-title":"Probiotics, Prebiotics, and Synbiotics: Bioactive Foods in Health Promotion"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1079\/NRR200479","article-title":"Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics","volume":"17","author":"Gibson","year":"2004","journal-title":"Nutr. Res. Rev."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.fshw.2016.04.001","article-title":"Moringa oleifera: A review on nutritive importance and its medicinal application","volume":"5","author":"Gopalakrishnan","year":"2016","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_32","first-page":"160","article-title":"Effect of microencapsulation on Saccharomyces cerevisiae var. boulardii viability in the gastrointestinal tract and level of some blood biochemical factors in wistar rats","volume":"11","author":"Nikaein","year":"2019","journal-title":"Iran. J. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"108966","DOI":"10.1016\/j.ijfoodmicro.2020.108966","article-title":"Application of essential oils as antimicrobial agents against spoilage and pathogenic microorganisms in meat products","volume":"337","author":"Pateiro","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1177\/1082013219842469","article-title":"Microencapsulation ofMoringa oleiferaleaf extracts with vegetable protein as wall materials","volume":"25","author":"Airouyuwa","year":"2019","journal-title":"Food Sci. Technol. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1007\/s12393-014-9106-7","article-title":"Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds","volume":"7","author":"Komes","year":"2015","journal-title":"Food Eng. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.foodchem.2018.09.086","article-title":"Encapsulation of stevia rebaudiana Bertoni aqueous crude extracts by ionic gelation\u2014Effects of alginate blends and gelling solutions on the polyphenolic profile","volume":"275","author":"Arriola","year":"2019","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Rosell, J.M., la Fuente, D., and Fernando, L. (2018). Mastitis on rabbit farms: Prevalence and risk factors. Animals, 8.","DOI":"10.3390\/ani8060098"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1080\/01652176.2015.1063023","article-title":"Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: A comprehensive review","volume":"35","author":"Dhama","year":"2015","journal-title":"Veter. Q."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1016\/j.ijbiomac.2018.06.151","article-title":"Antibacterial and antifungal effects of chitosan nanoparticles on tissue conditioners of complete dentures","volume":"118","author":"Mousavi","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"57","DOI":"10.3389\/fmicb.2019.00057","article-title":"Benefits and Inputs From Lactic Acid Bacteria and Their Bacteriocins as Alternatives to Antibiotic Growth Promoters During Food-Animal Production","volume":"10","author":"Belguesmia","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"303","DOI":"10.5713\/ajas.2011.r.05","article-title":"Feed Resources for Animals in Asia: Issues, Strategies for Use, Intensification and Integration for Increased Productivity","volume":"24","author":"Devendra","year":"2011","journal-title":"Asian-Australas. J. Anim. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"10480","DOI":"10.3390\/molecules190710480","article-title":"Antioxidant, Antimicrobial and Phytochemical Variations in Thirteen Moringa oleifera Lam","volume":"19","author":"Ndhlala","year":"2014","journal-title":"Cultivars. Molecules"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1148\/radiol.2313030126","article-title":"Penetrating Torso Trauma: Triple-Contrast Helical CT in Peritoneal Violation and Organ Injury\u2014A Prospective Study in 200 Patients","volume":"231","author":"Shanmuganathan","year":"2004","journal-title":"Radiology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"58","DOI":"10.3382\/ps.2009-00308","article-title":"Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition","volume":"89","author":"Mountzouris","year":"2010","journal-title":"Poult. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.ijmecsci.2016.08.015","article-title":"Numerical prediction of springback and ductile damage in rubber-pad forming process of aluminum sheet metal","volume":"117","author":"Belhassen","year":"2016","journal-title":"Int. J. Mech. Sci."},{"key":"ref_46","first-page":"35","article-title":"The effect of probiotics on animal health","volume":"43","author":"Corcionivoschi","year":"2010","journal-title":"Sci. Pap. Anim. Sci. Biotechnol."},{"key":"ref_47","unstructured":"Lee, A.J. (2019). U-Statistics: Theory and Practice, Routledge."},{"key":"ref_48","first-page":"1","article-title":"Haematotoxicity of dietary fumonisin B1 in growing pigs","volume":"7","author":"Gbore","year":"2010","journal-title":"ASSET Int. J. Ser. B"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Abdelnour, S., Al-Gabri, N., Hashem, N., and Gonzalez-Bulnes, A. (2021). Supplementation with Proline Improves Haemato-Biochemical and Reproductive Indicators in Male Rabbits Affected by Environmental Heat-Stress. Animals, 11.","DOI":"10.3390\/ani11020373"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/23\/4191\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:37:49Z","timestamp":1760168269000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/23\/4191"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,30]]},"references-count":49,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["polym13234191"],"URL":"https:\/\/doi.org\/10.3390\/polym13234191","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,30]]}}}