{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:51:54Z","timestamp":1791942714094,"version":"4.3.4"},"reference-count":41,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>The clinical use of platelet lysate (PL) in the treatment of wounds is limited by its rapid degradation by proteases at the tissue site. This research aims to develop a chitosan (CS) and kenaf nanocrystalline cellulose (NCC) hydrogel composite, which intend to stabilize PL and control its release onto the wound site for prolonged action. NCC was synthesized from raw kenaf bast fibers and incorporated into the CS hydrogel. The physicochemical properties, in vitro cytocompatibility, cell proliferation, wound scratch assay, PL release, and CS stabilizing effect of the hydrogel composites were analyzed. The study of swelling ratio (&gt;1000%) and moisture loss (60\u201390%) showed the excellent water retention capacity of the CS-NCC-PL hydrogels as compared with the commercial product. In vitro release PL study (flux = 0.165 mg\/cm2\/h) indicated that NCC act as a nanofiller and provided the sustained release of PL compared with the CS hydrogel alone. The CS also showed the protective effect of growth factor (GF) present in PL, thereby promoting fast wound healing via the formulation. The CS-NCC hydrogels also augmented fibroblast proliferation in vitro and enhanced wound closures over 72 h. This study provides a new insight on CS with renewable source kenaf NCC as a nanofiller as a potential autologous PL wound therapy.<\/jats:p>","DOI":"10.3390\/polym13244392","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T21:47:36Z","timestamp":1639604856000},"page":"4392","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Chitosan Reinforced with Kenaf Nanocrystalline Cellulose as an Effective Carrier for the Delivery of Platelet Lysate in the Acceleration of Wound Healing"],"prefix":"10.3390","volume":"13","author":[{"given":"Payal","family":"Bhatnagar","sequence":"first","affiliation":[{"name":"Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9045-5145","authenticated-orcid":false,"given":"Jia Xian","family":"Law","sequence":"additional","affiliation":[{"name":"Centre for Tissue Engineering & Regenerative Medicine, 12th Floor, Clinical Block, UKM Medical Centre, Jalan Yaa\u2019cob Latif, Cheras, Kuala Lumpur 56000, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiow-Fern","family":"Ng","sequence":"additional","affiliation":[{"name":"Centre for Drug Delivery Technology, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.jcis.2017.01.077","article-title":"Functionalization of cellulose nanocrystals for advanced applications","volume":"494","author":"Tang","year":"2017","journal-title":"J. Colloid Int. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"309","DOI":"10.17576\/mjas-2016-2002-12","article-title":"Penghasilan nano-gentian selulosa (CNF) diperolehi daripada gentian kulit kenaf dan potensinya sebagai penyokong pemegunan enzim","volume":"20","author":"Sulaiman","year":"2016","journal-title":"Malay. J. Anal. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.indcrop.2015.05.029","article-title":"Optimization of high pressure homogenization parameters for the isolation of cellulosic nanofibers using response surface methodology","volume":"74","author":"Davoudpour","year":"2015","journal-title":"Ind. Crop. Prod."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/j.jtv.2017.05.003","article-title":"Platelet-rich plasma with keratinocytes and fibroblasts enhance healing of full-thickness wounds","volume":"26","author":"Law","year":"2017","journal-title":"J. Tissue Viability"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"15","DOI":"10.12968\/jowc.2019.28.1.15","article-title":"Use of platelet-rich plasma and platelet-derived patches to treat chronic wounds","volume":"28","author":"Tsai","year":"2019","journal-title":"J. Wound Care"},{"key":"ref_6","first-page":"332","article-title":"Preparation and characterization of a hemostatic porous platelet-rich plasma chitosan\/silk fibroin wound dressing","volume":"36","author":"Han","year":"2020","journal-title":"Chin. J. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1089\/wound.2014.0540","article-title":"The roles of growth factors in keratinocyte migration","volume":"4","author":"Seeger","year":"2015","journal-title":"Adv. Wound Care"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.jprot.2017.07.017","article-title":"Mini-encyclopaedia of the wound healing\u2014Opportunities for integrating multi-omic approaches into medical practice","volume":"188","author":"Golubnitschaja","year":"2018","journal-title":"J. Proteom."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/j.carbpol.2013.06.044","article-title":"Chitosan stabilizes platelet growth factors and modulates stem cell differentiation toward tissue regeneration","volume":"98","author":"Busilacchi","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1586\/eri.11.59","article-title":"Chitosan preparations for wounds and burns: Antimicrobial and wound-healing effects","volume":"309","author":"Dai","year":"2011","journal-title":"Exp. Rev. Anti-Inf. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1016\/j.progpolymsci.2011.02.001","article-title":"Chitosan\u2014A versatile semi-synthetic polymer in biomedical applications","volume":"36","author":"Dash","year":"2011","journal-title":"Prog. Polym. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/j.apsb.2012.07.004","article-title":"Modified chitosan hydrogels as drug delivery and tissue engineering systems: Present status and applications","volume":"2","author":"Giri","year":"2012","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.ejpb.2015.08.004","article-title":"Chitosan as a starting material for wound healing applications","volume":"97","author":"Patrulea","year":"2015","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ijpharm.2012.07.056","article-title":"\u201cSponge-like\u201d dressings based on biopolymers for the delivery of platelet lysate to skin chronic wounds","volume":"440","author":"Rossi","year":"2013","journal-title":"Int. J. Pharm."},{"key":"ref_15","first-page":"29","article-title":"effect of platelet rich plasma combined with chitosan biodegradable film on full-thickness wound healing in rat model","volume":"4","author":"Mohammadi","year":"2016","journal-title":"Bull. Emerg. Traum."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5433","DOI":"10.1016\/j.biomaterials.2005.01.023","article-title":"Synergistic platelet integrin signaling and factor XII activation in poly-N-acetyl glucosamine fiber-mediated hemostasis","volume":"26","author":"Fischer","year":"2005","journal-title":"Biomaterials"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e435","DOI":"10.1038\/am.2017.171","article-title":"Novel biomaterial strategies for controlled growth factor delivery for biomedical applications","volume":"9","author":"Wang","year":"2017","journal-title":"NPG Asia Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1021\/acssuschemeng.9b05317","article-title":"Tunable biomimetic hydrogels from silk fibroin and nanocellulose","volume":"8","author":"Dorishetty","year":"2020","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.carbpol.2016.04.064","article-title":"Development of nanocellulose scaffolds with tunable structures to support 3D cell culture","volume":"148","author":"Liu","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_20","first-page":"1","article-title":"Preparation and characterization of cellulose nanocrystal extracted from Calotropis procera biomass","volume":"45","author":"Song","year":"2019","journal-title":"Bioresour. Bioprocess."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1002\/jbm.b.32806","article-title":"Platelet-rich plasma-loaded chitosan scaffolds: Preparation and growth factor release kinetics","volume":"101B","author":"Kutlu","year":"2013","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Doench, I., Tran, T.A., David, L., Montembault, A., Viguier, E., Gorzelanny, C.G., Sudre, T., Cachon, M., Louback-Mohamed, M., and Horbelt, N. (2019). Cellulose nanofiber-reinforced chitosan hydrogel composites for intervertebral disc tissue repair. Biomimetics, 4.","DOI":"10.3390\/biomimetics4010019"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1016\/j.eurpolymj.2019.04.021","article-title":"Synthesis, characterization and cytotoxicity studies of nanocrystalline cellulose from the production waste of rubber-wood and kenaf-bast fibers","volume":"116","author":"Tuerxun","year":"2019","journal-title":"Eur. Polym. J."},{"key":"ref_24","unstructured":"Sri Aprilia, N.A., and Arahman, N. (2020). Properties of nanocrystalline cellulose from pineapple crown leaf waste. IOP Conference Series: Materials Science and Engineering, IOP Publishing."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/j.carbpol.2017.04.096","article-title":"Characterization of a novel natural cellulosic fiber from Juncus effusus L.","volume":"171","author":"Maache","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.jep.2016.05.032","article-title":"Treatment for diabetic ulcer wounds using a fern tannin optimized hydrogel formulation with antibacterial and antioxidative properties","volume":"189","author":"Lai","year":"2016","journal-title":"J. Ethnopharmacol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"39532","DOI":"10.1021\/acsami.8b16645","article-title":"Cellular and molecular interaction of human dermal fibroblasts with bacterial nanocellulose composite hydrogel for tissue regeneration","volume":"10","author":"Fauzi","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1007\/s12663-015-0768-3","article-title":"Growth factor measurement and histological analysis in platelet rich fibrin: A pilot study","volume":"14","author":"Nishimoto","year":"2015","journal-title":"J. Maxillofac. Oral Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1007\/s10570-011-9546-7","article-title":"Characteristics of cellulose nanofibers isolated from rubberwood and empty fruit bunches of oil palm using chemo-mechanical process","volume":"18","author":"Jonoobi","year":"2011","journal-title":"Cellulose"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s10570-005-9039-7","article-title":"Dufresne, Sisal cellulose whiskers reinforced polyvinyl acetate nanocomposites","volume":"13","author":"Thielemans","year":"2006","journal-title":"Cellulose"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1016\/j.carbpol.2017.11.045","article-title":"Isolation and characterization of nanocrystalline cellulose from sugar palm fibres (Arenga Pinnata)","volume":"181","author":"Ilyas","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_32","first-page":"461","article-title":"Impact of surface electric properties of carbon-based thin films on platelets activation for nano-medical and nano-sensing applications","volume":"3","author":"Karagkiozaki","year":"2008","journal-title":"Int. J. Nanomed."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.carbpol.2013.09.106","article-title":"Kenaf bast cellulosic fibers hierarchy: A comprehensive approach from micro to nano","volume":"101","author":"Karimi","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Khan, A., Jawaid, M., Kia Kian, L., Khan, A.A.P., and Asiri, A.M. (2021). Isolation and production of nanocrystalline cellulose from conocarpus fiber. Polymers, 13.","DOI":"10.3390\/polym13111835"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"113934","DOI":"10.1016\/j.indcrop.2021.113934","article-title":"Using microwave assisted organic acid treatment to separate cellulose fiber and lignin from kenaf bast","volume":"171","author":"Lv","year":"2021","journal-title":"Ind. Crop. Prod."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1016\/j.compositesa.2007.12.001","article-title":"Influence of various chemical treatments on the composition and structure of hemp fibres","volume":"39","author":"Sedan","year":"2008","journal-title":"Compos. Part A Appl. Sci. Manuf."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.carbpol.2006.02.032","article-title":"Kenaf xylan\u2014A source of biologically active acidic oligosaccharides","volume":"66","author":"Nacos","year":"2006","journal-title":"Carbohydr. Polym."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"27013","DOI":"10.1039\/C9RA03972D","article-title":"Biocompatible chitosan-collagen-hydroxyapatite nanofibers coated with platelet-rich plasma for regenerative engineering of the rotator cuff of the shoulder","volume":"9","author":"Tang","year":"2019","journal-title":"RSC Adv."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Song, W., Xu, J., Gao, L., Zhang, Q., and Tong, J. (2021). Preparation of Freeze-Dried Porous Chitosan Microspheres for the Removal of Hexavalent Chromium. Appl. Sci., 11.","DOI":"10.3390\/app11094217"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1007\/s10570-017-1251-8","article-title":"Preparation and characterization of nanocellulose reinforced semi-interpenetrating polymer network of chitosan hydrogel","volume":"24","author":"Sampath","year":"2017","journal-title":"Cellulose"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.3892\/etm.2017.4041","article-title":"Feasibility of improving platelet-rich plasma therapy by using chitosan with high platelet activation ability","volume":"13","author":"Hattori","year":"2017","journal-title":"Exp. Ther. Med."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/24\/4392\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:48:38Z","timestamp":1760168918000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/13\/24\/4392"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,15]]},"references-count":41,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["polym13244392"],"URL":"https:\/\/doi.org\/10.3390\/polym13244392","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,15]]}}}