{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:13:14Z","timestamp":1789542794439,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006541","name":"Comunidad de Madrid","doi-asserted-by":"publisher","award":["B2017\/BMD-3804"],"award-info":[{"award-number":["B2017\/BMD-3804"]}],"id":[{"id":"10.13039\/501100006541","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Chronic infections are one of the most serious adverse outcomes of prosthetic surgery. Prosthetic revision surgery using a bone cement loaded with antibiotics between the two stages of the surgery is commonly performed. However, this method often fails to reach the minimum inhibitory concentration and promotes antibiotic resistance, thus emphasizing the need for improving the current available therapies. Materials and methods: In this study, we performed a study of the in vivo response of a polymer-based construct of poly (lactic-co-glycolic acid) (PLGA) in the solid phase of Palacos R\u00ae in combination with vancomycin, daptomycin, and\/or linezolid. To test its effectiveness, we applied an in vivo model, using both histological and immunohistochemical analyses to study the bone tissue. Results: The presence of PLGA in the combination of vancomycin with daptomycin showed the most promising results regarding the preservation of bone cytoarchitecture and S. aureus elimination. Conversely, the combination of vancomycin plus linezolid was associated with a loss of bone cytoarchitecture, probably related to an increased macrophage response and inefficient antimicrobial activity. Conclusions: The modification of Palacos R\u00ae bone cement with PLGA microspheres and its doping with the antibiotic daptomycin in combination with vancomycin improve the tissue response to bone infection.<\/jats:p>","DOI":"10.3390\/polym14050888","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T00:53:26Z","timestamp":1645664006000},"page":"888","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Modification of the Polymer of a Bone Cement with Biodegradable Microspheres of PLGA and Loading with Daptomycin and Vancomycin Improve the Response to Bone Tissue Infection"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0050-4634","authenticated-orcid":false,"given":"Joaquin","family":"Garc\u00eda-Garc\u00eda","sequence":"first","affiliation":[{"name":"Service of Orthopedic Surgery of University Hospital Principe de Asturias, 28805 Alcal\u00e1 de Henares, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galo","family":"Azuara","sequence":"additional","affiliation":[{"name":"Service of Traumatology of University Hospital of Guadalajara, 19002 Guadalajara, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4494-6397","authenticated-orcid":false,"given":"Oscar","family":"Fraile-Martinez","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6016-7855","authenticated-orcid":false,"given":"Cielo","family":"Garc\u00eda-Montero","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Angel","family":"\u00c1lvarez-Mon","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ru\u00edz-D\u00edez","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1309-7510","authenticated-orcid":false,"given":"Melchor","family":"\u00c1lvarez-Mon","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"},{"name":"Immune System Diseases-Rheumatology, Oncology Service an Internal Medicine, University Hospital Pr\u00edncipe de Asturias, (CIBEREHD), 28806 Alcala de Henares, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2419-2476","authenticated-orcid":false,"given":"Julia","family":"Buj\u00e1n","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0904-6198","authenticated-orcid":false,"given":"Natalio","family":"Garc\u00eda-Honduvilla","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2588-1708","authenticated-orcid":false,"given":"Miguel A.","family":"Ortega","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Medical Specialities, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Ram\u00f3n y Cajal Institute of Sanitary Research (IRYCIS), 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basilio","family":"De la Torre","sequence":"additional","affiliation":[{"name":"Department of Surgery, Medical and Social Sciences, Faculty of Medicine and Health Sciences, University of Alcal\u00e1, Alcal\u00e1 de Henares, 28801 Madrid, Spain"},{"name":"Service of Traumatology of University Hospital Ram\u00f3n y Cajal, 28034 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.3928\/01477447-20140401-08","article-title":"Infection After Primary Total Hip 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