{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T20:16:24Z","timestamp":1781554584911,"version":"3.54.5"},"reference-count":36,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2022,8,4]],"date-time":"2022-08-04T00:00:00Z","timestamp":1659571200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d","award":["No. 14.604.21.0001"],"award-info":[{"award-number":["No. 14.604.21.0001"]}]},{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d","award":["No. 2755"],"award-info":[{"award-number":["No. 2755"]}]},{"name":"National Research Center \u201cKurchatov Institute\u201d","award":["No. 14.604.21.0001"],"award-info":[{"award-number":["No. 14.604.21.0001"]}]},{"name":"National Research Center \u201cKurchatov Institute\u201d","award":["No. 2755"],"award-info":[{"award-number":["No. 2755"]}]},{"name":"Academy of Finland (project 351068)","award":["No. 14.604.21.0001"],"award-info":[{"award-number":["No. 14.604.21.0001"]}]},{"name":"Academy of Finland (project 351068)","award":["No. 2755"],"award-info":[{"award-number":["No. 2755"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Aim: In this study, we seek to check if recombinant spidroin rS1\/9 is applicable for cell-engineering construct development. Novel technologies of cell and tissue engineering are relevant for chronic liver failure management. Liver regeneration may represent one of the possible treatment options if a cell-engineered construct (CEC) is used. Nowadays, one can see the continuous study of various matrices to create an appropriate CEC. Materials and Methods: We have adhered allogenic liver cells and multipotent mesenchymal bone marrow stem cells (MMSC BM) to a microgel with recombinant spidroin rS1\/9. Then we have studied the developed implantable CEC in a rat model (n = 80) of chronic liver failure achieved by prolonged poisoning with carbon tetrachloride. Results: Our results demonstrate that the CECs change the values of biochemical tests and morphological parameters in chronic liver failure in rats. Conclusion: We consider there to be a positive effect from the microgel-based CECs with recombinant spidroin rS1\/9 in the treatment of chronic liver failure.<\/jats:p>","DOI":"10.3390\/polym14153179","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T02:12:39Z","timestamp":1659665559000},"page":"3179","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Recombinant Spidroin Microgel as the Base of Cell-Engineered Constructs Mediates Liver Regeneration in Rats"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4627-6208","authenticated-orcid":false,"given":"Murat","family":"Shagidulin","sequence":"first","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"},{"name":"Federal State Autonomous Educational Institution of Higher Education, \u201cI.M. Sechenov First Moscow State Medical University\u201d of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina","family":"Onishchenko","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasiia","family":"Grechina","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Educational Institution of Higher Education, \u201cI.M. Sechenov First Moscow State Medical University\u201d of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla","family":"Nikolskaya","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3574-4013","authenticated-orcid":false,"given":"Mikhail","family":"Krasheninnikov","sequence":"additional","affiliation":[{"name":"Research and Education Resourse Center, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0102-5491","authenticated-orcid":false,"given":"Aleksey","family":"Lyundup","sequence":"additional","affiliation":[{"name":"Research and Education Resourse Center, Peoples Friendship University of Russia (RUDN University), 117198 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Volkova","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Mogeiko","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem","family":"Venediktov","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Educational Institution of Higher Education, \u201cI.M. Sechenov First Moscow State Medical University\u201d of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7782-3468","authenticated-orcid":false,"given":"Gennadii","family":"Piavchenko","sequence":"additional","affiliation":[{"name":"Federal State Autonomous Educational Institution of Higher Education, \u201cI.M. Sechenov First Moscow State Medical University\u201d of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubov","family":"Davydova","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla","family":"Ramonova","sequence":"additional","affiliation":[{"name":"Bioengineering Department, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Bogush","sequence":"additional","affiliation":[{"name":"National Research Center \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Gautier","sequence":"additional","affiliation":[{"name":"Federal State Budgetary Institution \u201cShumakov National Medical Research Center of Transplantology and Artificial Organs\u201d of the Ministry of Health of the Russian Federation, 123182 Moscow, Russia"},{"name":"Federal State Autonomous Educational Institution of Higher Education, \u201cI.M. Sechenov First Moscow State Medical University\u201d of the Ministry of Health of the Russian Federation (Sechenov University), 119435 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,4]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, May 30). World Health Statistics 2021: Monitoring Health for the SDGs, Sustainable Development Goals, Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/342703."},{"key":"ref_2","unstructured":"(2022, May 30). Available online: www.eurotransplant.nl\/files\/statistics."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Mokdad, A., Lopez, A., Shahraz, S., Lozano, R., Mokdad, A., Stanaway, J., Murray, C., and Naghavi, M. (2014). Liver cirrhosis mortality in 187 countries between 1980 and 2010: A systematic analysis. 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