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Remarkably, extracellular vesicles (EVs) derived from MSCs display similar functions as their parental cells, mitigating tissue damage in lung diseases. However, recent data have shown that ECM-derived hydrogels could release other resident vesicles similar to EVs. Here, we aim to better understand the contribution of EVs and ECM-vesicles released from MSCs and\/or lung-derived hydrogel (L-HG) in lung repair by using an in vitro lung injury model. L-HG derived-vesicles and MSCs EVs cultured either in L-HG or conventional plates were isolated and characterized. The therapeutic capacity of vesicles obtained from each experimental condition was tested by using an alveolar epithelial wound-healing assay. The number of ECM-vesicles released from acellular L-HG was 10-fold greater than EVs from conventional MSCs cell culture revealing that L-HG is an important source of bioactive vesicles. MSCs-derived EVs and L-HG vesicles have similar therapeutic capacity in lung repair. However, when wound closure rate was normalized by total proteins, the MSCs-derived EVs shows higher therapeutic potential to those released by L-HG. The EVs released from L-HG must be considered when HG is used as substrate for cell culture and EVs isolation.<\/jats:p>","DOI":"10.3390\/polym14224907","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T02:32:16Z","timestamp":1668479536000},"page":"4907","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Lung Extracellular Matrix Hydrogels-Derived Vesicles Contribute to Epithelial Lung Repair"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8825-9436","authenticated-orcid":false,"given":"Anna","family":"Ulldemolins","sequence":"first","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9275-3047","authenticated-orcid":false,"given":"Alicia","family":"Jurado","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Herranz-Diez","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7666-110X","authenticated-orcid":false,"given":"N\u00faria","family":"Gavara","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"},{"name":"The Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Otero","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"},{"name":"The Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain"},{"name":"CIBER de Enfermedades Respiratorias, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9084-7824","authenticated-orcid":false,"given":"Ramon","family":"Farr\u00e9","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"},{"name":"CIBER de Enfermedades Respiratorias, 28029 Madrid, Spain"},{"name":"Institut d\u2019Investigacions Biom\u00e8diques August Pi i Sunyer, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1998-9379","authenticated-orcid":false,"given":"Isaac","family":"Almendros","sequence":"additional","affiliation":[{"name":"Unitat de Biof\u00edsica i Bioenginyeria, Facultat de Medicina i Ci\u00e8ncies de la Salut, Universitat de Barcelona, 08036 Barcelona, Spain"},{"name":"CIBER de Enfermedades Respiratorias, 28029 Madrid, Spain"},{"name":"Institut d\u2019Investigacions Biom\u00e8diques August Pi i Sunyer, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1080\/15583724.2015.1040552","article-title":"A Review on Extracellular Matrix Mimicking Strategies for an Artificial Stem Cell Niche","volume":"55","author":"Jhala","year":"2015","journal-title":"Polym. 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Part A"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/14\/22\/4907\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:17:36Z","timestamp":1760145456000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/14\/22\/4907"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,14]]},"references-count":37,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["polym14224907"],"URL":"https:\/\/doi.org\/10.3390\/polym14224907","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,14]]}}}