{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:58:43Z","timestamp":1787630323850,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,12]],"date-time":"2023-02-12T00:00:00Z","timestamp":1676160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006070","name":"University of the Andes","doi-asserted-by":"publisher","award":["PR.3.2017.4791"],"award-info":[{"award-number":["PR.3.2017.4791"]}],"id":[{"id":"10.13039\/501100006070","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Antimicrobial resistance has been declared one of the top 10 global public health threats. Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of recurring skin and soft tissue infections in patients with chronic skin conditions such as diabetic foot infections, making the treatment of the ulcers challenging. Wound dressings combined with metal nanoparticles have been suggested to prevent and treat MRSA-infected wounds. However, these particles are commonly synthesized by chemical approaches. In this study, we developed bio-based silver (Bio-AgNPs) and copper oxide nanoparticles (CuONPs) polymer composites using a microbially produced polyester from the Polyhydroxyalkanoates (PHAs) family. Poly(3-hydroxyoctanoate)-co-(3-hydroxyhexanoate) (PHO) was synthesized by Pseudomonas putida and functionalized in-situ with Bio-AgNPs or ex-situ with CuONPs. PHO-CuONPs films did not inhibit MRSA growth, while a reduction of 6.0 log CFU\/mL was achieved with PHO-Bio-AgNPs synthesized from silver nitrate (AgNO3) solution at 3.5 mM. Exposure of human fibroblast cells (HFF-1) to the bioactive films did not induce notable cytotoxicity and genotoxicity, as seen by a viability higher than 79% and no significant changes in basal DNA damage. However, exposure to PHO-Bio-AgNPs induced oxidative DNA damage in HFF-1 cells. No hemolytic potential was observed, while platelet aggregation was promoted and desired for wound healing. Here we demonstrate the biosynthesis of polymer-nanoparticle composites and their potential as bioactive films for MRSA treatment.<\/jats:p>","DOI":"10.3390\/polym15040920","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T05:59:40Z","timestamp":1676267980000},"page":"920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Microbially Synthesized Polymer-Metal Nanoparticles Composites as Promising Wound Dressings to Overcome Methicillin-Resistance Staphylococcus aureus Infections"],"prefix":"10.3390","volume":"15","author":[{"given":"Jennifer","family":"Balcucho","sequence":"first","affiliation":[{"name":"Nanotechnology and Applied Microbiology Research Group (NANOBIOT), Department of Biological Sciences, University of the Andes, Bogota 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0844-8788","authenticated-orcid":false,"given":"Diana M.","family":"Narv\u00e1ez","sequence":"additional","affiliation":[{"name":"Human Genetics Laboratory, Department of Biological Sciences, University of the Andes, Bogota 111711, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8544-3633","authenticated-orcid":false,"given":"Natalia A.","family":"Tarazona","sequence":"additional","affiliation":[{"name":"Institute of Active Polymers, Helmholtz-Zentrum Hereon, Kantstra\u00dfe 55, 14513 Teltow, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinneth Lorena","family":"Castro-Mayorga","sequence":"additional","affiliation":[{"name":"Agricultural Bioproduct and Bioprocess Research Group, Department of Bioproduct, Colombian Corporation of Agricultural Research, Agrosavia, Mosquera 250047, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.jaip.2014.04.007","article-title":"Recurrent MRSA Skin Infections in Atopic Dermatitis","volume":"2","author":"Ong","year":"2014","journal-title":"J. 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