{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:35:28Z","timestamp":1790422528944,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T00:00:00Z","timestamp":1679011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamenteel Onderzoek der Materie (FOM)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>3D printing is gaining traction in research and development as a way to quickly, cheaply, and easily manufacture polydimethylsiloxane (PDMS) molds. The most commonly used method is resin printing, which is relatively expensive and requires specialized printers. This study shows that polylactic acid (PLA) filament printing is a cheaper, more readily available alternative to resin printing, that does not inhibit the curing of PDMS. As a proof of concept, a PLA mold for PDMS-based wells was designed, and 3D printed. We introduce an effective method to smooth the printed PLA mold, based on chloroform vapor treatment. After this chemical post-processing step, the smoothened mold was used to cast a ring of PDMS prepolymer. The PDMS ring was attached to a glass coverslip after oxygen plasma treatment. The PDMS\u2013glass well showed no leakage and was well suited to its intended use. When used for cell culturing, monocyte-derived dendritic cells (moDCs) showed no morphological anomalies, as tested by confocal microscopy, nor did they show an increase in cytokines, as tested using ELISA. This underlines the versatility and strength of PLA filament printing and exemplifies how it can be valuable to a researcher\u2019s toolset.<\/jats:p>","DOI":"10.3390\/polym15061498","type":"journal-article","created":{"date-parts":[[2023,3,17]],"date-time":"2023-03-17T02:29:59Z","timestamp":1679020199000},"page":"1498","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["PLA 3D Printing as a Straightforward and Versatile Fabrication Method for PDMS Molds"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4481-3498","authenticated-orcid":false,"given":"Guus","family":"van der Borg","sequence":"first","affiliation":[{"name":"Molecular Biophysics, Zernike Institute for Advanced Materials, Rijksuniversiteit Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Warner","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, Rijksuniversiteit Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melina","family":"Ioannidis","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, Rijksuniversiteit Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-6735","authenticated-orcid":false,"given":"Geert","family":"van den Bogaart","sequence":"additional","affiliation":[{"name":"Department of Molecular Immunology, Groningen Biomolecular Sciences and Biotechnology Institute, Rijksuniversiteit Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wouter H.","family":"Roos","sequence":"additional","affiliation":[{"name":"Molecular Biophysics, Zernike Institute for Advanced Materials, Rijksuniversiteit Groningen, 9747 AG Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/s10544-005-6070-2","article-title":"Characterization of polydimethylsiloxane (PDMS) properties for biomedical micro\/nanosystems","volume":"7","author":"Mata","year":"2005","journal-title":"Biomed. 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