{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T23:56:43Z","timestamp":1782172603998,"version":"3.54.5"},"reference-count":55,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology, ROC","award":["110-2221-E-011-011-MY3"],"award-info":[{"award-number":["110-2221-E-011-011-MY3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>A hole array was fabricated via photolithography to wet the bottoms of holes using oxygen plasma. Amide-terminated silane, a water immiscible compound before hydrolysis, was evaporated for deposition on the plasma-treated hole template surface. The silane compound was hydrolyzed along the edges of circular sides of the hole bottom to form a ring of an initiator after halogenation. Poly(methacrylic acid) (PMAA) was grafted from the ring of the initiator to attract Ag clusters (AgCs) as AgC\u2013PMAA hybrid ring (SPHR) arrays via alternate phase transition cycles. The SPHR arrays were modified with a Yersinia pestis antibody (abY) to detect the antigen of Yersinia pestis (agY) for plague diagnosis. The binding of the agY onto the abY-anchored SPHR array resulted in a geometrical change from a ring to a two-humped structure. The reflectance spectra could be used to analyze the AgC attachment and the agY binding onto the abY-anchored SPHR array. The linear range between the wavelength shift and agY concentration from 30 to 270 pg mL\u22121 was established to obtain the detection limit of ~12.3 pg mL\u22121. Our proposed method provides a novel pathway to efficiently fabricate a ring array with a scale of less than 100 nm, which demonstrates excellent performance in preclinical trials.<\/jats:p>","DOI":"10.3390\/polym15081919","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T01:36:45Z","timestamp":1681781805000},"page":"1919","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Designable Poly(methacrylic Acid)\/Silver Cluster Ring Arrays as Reflectance Spectroscopy-Based Biosensors for Label-Free Plague Diagnosis"],"prefix":"10.3390","volume":"15","author":[{"given":"Chih-Wei","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Neurosurgery, Department of Surgery, Chi Mei Medical Center, Tainan 710, Taiwan"},{"name":"Department of Occupational Safety and Health, Institute of Industrial Safety and Disaster Prevention, College of Sustainable Environment, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan"},{"name":"Department of Materials and Science Engineering, National Taiwan University of Science and Technology, 43, Sec. 4, Keelung Road, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5750-6470","authenticated-orcid":false,"given":"Shih-Hsun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, National Yang Ming Chiao Tung University, No. 1001, Daxue Rd. East Dist., Hsinchu City 300093, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8062-8708","authenticated-orcid":false,"given":"Chih-Feng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, i-Center for Advanced Science and Technology (iCAST), National Chung Hsing University, Taichung 40227, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3670-6710","authenticated-orcid":false,"given":"Jem-Kun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Materials and Science Engineering, National Taiwan University of Science and Technology, 43, Sec. 4, Keelung Road, Taipei 106, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"46519","DOI":"10.1021\/acsami.0c12525","article-title":"Grating-Coupled Surface Plasmon-Polariton Sensing at a Flat Metal-Analyte Interface in a Hybrid-Configuration","volume":"12","author":"Joseph","year":"2020","journal-title":"ACS Appl. 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