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However, in the case of Lophira alata (L. alata), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin\u2019s autofluorescence in L. alata by conducting associated experiments. Notably, the autofluorescence image of L. alata observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of L. alata. Remarkably, when subjected to a benzene\u2013alcohol extraction treatment, the autofluorescence of L. alata becomes progressively enhanced under a fluorescence microscope. Additionally, UV\u2013Vis absorption spectra demonstrate that the extractives derived from L. alata exhibit strong light absorption within the wavelength range of 200\u2013500 nm. This suggests that the abundant extractives in L. alata are probably responsible for the autofluorescence quenching observed in the cell walls. Moreover, the presence and quantity of these extractives have a significant impact on the fluorescence intensity of lignin in wood, resulting in a significant decrease therein. In future studies, it would be interesting to explore the role of complex compounds such as polyphenols or terpenoids, which are present in the abundant extractives, in interfering with the fluorescence quenching of lignin in L. alata.<\/jats:p>","DOI":"10.3390\/polym15153269","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"3269","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Improving the Autofluorescence of Lophira alata Woody Cells via the Removal of Extractives"],"prefix":"10.3390","volume":"15","author":[{"given":"Zhaoyang","family":"Yu","sequence":"first","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongnian","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3367-7398","authenticated-orcid":false,"given":"Jinbo","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"},{"name":"Department of Research and Development Center, Yihua Lifestyle Technology Co., Ltd., Shantou 515834, China"},{"name":"Hunan Taohuajiang Bamboo Science & Technology Co., Ltd., Taojiang 413400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Chang","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonggang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongtao","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Research and Development Center, Yihua Lifestyle Technology Co., Ltd., Shantou 515834, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Han","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Taohuajiang Bamboo Science & Technology Co., Ltd., Taojiang 413400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong (Alice)","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Wood and Forest Sciences, Laval University, Quebec, QC G1V 0A6, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/124672","article-title":"Vital Autofluorescence: Application to the Study of Plant Living Cells","volume":"2012","author":"Roshchina","year":"2012","journal-title":"Int. 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