{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:41:44Z","timestamp":1780080104001,"version":"3.54.0"},"reference-count":68,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SEP through the PRODEP UABC-CA-307 project","award":["272894"],"award-info":[{"award-number":["272894"]}]},{"name":"CONAHCYT-PRONACES","award":["272894"],"award-info":[{"award-number":["272894"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>In this study, we synthesized and characterized pH-responsive Chitosan\u2013AgCl-doped ZnO hybrid hydrogels and evaluated their potential for loading aquaculture bioactive compounds, and assessed their antimicrobial properties against a threatening pathogen associated with disease across a broad spectrum of warm water fish and invertebrates. Hydrogel characterization consisted of assessing morphology via SEM, composition via EDS, hydrogels\u2019 network components interactions via FT-IR and pH response through swelling behavior determinations. The swelling characterization of the synthesized hydrogels demonstrated a pH-responsive behavior, showing that low pH values caused the hydrogel polymeric network to expand and capture more of the aqueous solution. These characteristics make the synthesized hydrogels suitable for the encapsulation and controlled release of drugs and bioactive compounds in aquaculture. Chitosan_ZnO hybrid hydrogels showed great antimicrobial activity against Vibrio harveyi, even better than that of loaded PB hydrogels. Here, we provide evidence for the potential capacity of Chitosan_ZnO hybrid hydrogels for the preventive and curative treatment of diseases that impact aquaculture animal health and prevent drug resistance by bacteria.<\/jats:p>","DOI":"10.3390\/polym15204105","type":"journal-article","created":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T08:32:57Z","timestamp":1697445177000},"page":"4105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["pH-Responsive Chitosan-Doped ZnO Hybrid Hydrogels for the Encapsulation of Bioactive Compounds in Aquaculture"],"prefix":"10.3390","volume":"15","author":[{"given":"Samuel","family":"S\u00e1nchez-Serrano","sequence":"first","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela J.","family":"Gonz\u00e1lez-M\u00e9ndez","sequence":"additional","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 A.","family":"Olivas-Valdez","sequence":"additional","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalie","family":"Mill\u00e1n-Agui\u00f1aga","sequence":"additional","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viridiana","family":"Evangelista","sequence":"additional","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar E.","family":"Contreras","sequence":"additional","affiliation":[{"name":"Nanosciences and Nanotechnology Center, National Autonomus University of Mexico, Ensenada 22800, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlene N.","family":"Cardoza-Contreras","sequence":"additional","affiliation":[{"name":"Marine Sciences Faculty, Autonomous University of Baja California, Ensenada 22860, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/s41586-021-03308-6","article-title":"A 20-year retrospective review of global aquaculture","volume":"591","author":"Naylor","year":"2021","journal-title":"Nature"},{"key":"ref_2","unstructured":"FAO (2022). El Estado Mundial de la Pesca y la Acuicultura 2022: Hacia la Transformaci\u00f3n Azul, FAO."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21878","DOI":"10.1038\/s41598-020-78849-3","article-title":"Global trends in antimicrobial use in aquaculture","volume":"10","author":"Schar","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5432497","DOI":"10.1155\/2018\/5432497","article-title":"Maintenance of Fish Health in Aquaculture: Review of Epidemiological Approaches for Prevention and Control of Infectious Disease of Fish","volume":"2018","author":"Assefa","year":"2018","journal-title":"Vet. Med. Int."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1111\/raq.12344","article-title":"Antibiotic use in aquaculture, policies and regulation, health and environmental risks: A review of the top 15 major producers","volume":"12","author":"Lulijwa","year":"2020","journal-title":"Rev. Aquacult."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"11908","DOI":"10.1021\/acs.jafc.0c03996","article-title":"Antibiotics and Food Safety in Aquaculture","volume":"68","author":"Chen","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/j.jes.2021.06.026","article-title":"Metagenomic insights into the profile of antibiotic resistomes in sediments of aquaculture wastewater treatment system","volume":"113","author":"Chen","year":"2021","journal-title":"J. Environ. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Pepi, M., and Focardi, S. (2021). Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area. Int. J. Environ. Res. Public. Health, 18.","DOI":"10.3390\/ijerph18115723"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105551","DOI":"10.1016\/j.envint.2020.105551","article-title":"Distribution, combined pollution and risk assessment of antibiotics in typical marine aquaculture farms surrounding the Yellow Sea, North China","volume":"138","author":"Han","year":"2020","journal-title":"Environ. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1926337","DOI":"10.1080\/20964129.2021.1926337","article-title":"Antibiotic residues from aquaculture farms and their ecological risks in Southeast Asia: A case study from Malaysia","volume":"7","author":"Thiang","year":"2021","journal-title":"Ecosyst. Health Sustain."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"69241","DOI":"10.1007\/s11356-022-22319-y","article-title":"Antibiotic resistance in aquaculture and aquatic organisms: A review of current nanotechnology applications for sustainable management","volume":"29","author":"Okeke","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Yin, Y., Hu, B., Yuan, X., Cai, L., Gao, H., and Yang, Q. (2020). Nanogel: A Versatile Nano-Delivery System for Biomedical Applications. Pharmaceutics, 12.","DOI":"10.3390\/pharmaceutics12030290"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bordbar-Khiabani, A., and Gasik, M. (2022). Smart Hydrogels for Advanced Drug Delivery Systems. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23073665"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1177\/08853282221078107","article-title":"Injectable in situ intelligent thermo-responsive hydrogel with glycyrrhetinic acid-conjugated nano graphene oxide for chemo-photothermal therapy of malignant hepatocellular tumor","volume":"37","author":"Wang","year":"2022","journal-title":"J. Biomater. Appl."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1111\/raq.12250","article-title":"Encapsulation for delivering bioactives in aquaculture","volume":"11","author":"Masoomi","year":"2019","journal-title":"Rev. Aquac."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16071","DOI":"10.1038\/natrevmats.2016.71","article-title":"Designing hydrogels for controlled drug delivery","volume":"1","author":"Li","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"8205","DOI":"10.1039\/C5SM01789K","article-title":"Multifunctional hybrid nanogels for theranostic applications","volume":"11","year":"2015","journal-title":"Soft. Matter."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mao-Hua, C., Xiao-Yi, C., Luo-Qin, F., Wen-Lin, D., Xue, Y., Xiao-Zhou, M., and Pei-Yang, H. (2021). Design and Development of Hybrid Hydrogels for Biomedical Applications: Recent Trends in Anticancer Drug Delivery and Tissue Engineering. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.630943"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3390\/polym7030552","article-title":"Chitosan: Gels and Interfacial Properties","volume":"7","author":"Strand","year":"2015","journal-title":"Polymers"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Saeed, R.M., Dmour, I., and Taha, M.O. (2020). Stable Chitosan-Based Nanoparticles Using Polyphosphoric Acid or Hexametaphosphate for Tandem Ionotropic\/Covalent Crosslinking and Subsequent Investigation as Novel Vehicles for Drug Delivery. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00004"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s10311-020-00963-5","article-title":"Drug delivery applications of chitin and chitosan: A review","volume":"18","author":"Parhi","year":"2020","journal-title":"Environ. Chem. Lett."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"439","DOI":"10.3920\/BM2016.0177","article-title":"Effects of dietary chitosan on growth, lipid metabolism, immune response and antioxidant-related gene expression in Misgurnus anguillicaudatus","volume":"8","author":"Yan","year":"2017","journal-title":"Benef. Microbes"},{"key":"ref_23","unstructured":"FDA (2023, August 25). CFR\u2014Code of Federal Regulations Title 21, Chapter I, Subchapter A, Part 73, Subpart B, Available online: https:\/\/www.ecfr.gov\/current\/title-21."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1016\/j.aninu.2016.06.003","article-title":"Nano zinc, an alternative to conventional zinc as animal feed supplement: A review","volume":"2","author":"Swain","year":"2016","journal-title":"Anim. Nutr."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100082","DOI":"10.1016\/j.onano.2022.100082","article-title":"Synthesis, photocatalytic degradation and antibacterial properties of selenium or silver doped zinc oxide nanoparticles: A detailed review","volume":"8","author":"Ifijen","year":"2022","journal-title":"OpenNano"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100237","DOI":"10.1016\/j.apsadv.2022.100237","article-title":"Cytotoxicity and DNA fragmentation-mediated apoptosis response of hexagonal ZnO nanorods against human prostate cancer cells","volume":"9","author":"Yadav","year":"2022","journal-title":"Appl. Surf. Sci. Adv."},{"key":"ref_27","first-page":"125415","article-title":"Evaluation of antioxidant and anticancer properties of zinc oxide nanoparticles synthesized using Aspergillus niger extract","volume":"6","author":"Soltani","year":"2019","journal-title":"Mater. Res. Express"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.jphotobiol.2015.02.008","article-title":"Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract","volume":"146","author":"Nagajyothi","year":"2015","journal-title":"J. Photochem. Photobiol. B Biol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Li, Y., Liao, C., and Tjong, S.C. (2020). Recent Advances in Zinc Oxide Nanostructures with Antimicrobial Activities. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21228836"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1039\/C9RA09512H","article-title":"Antibacterial potential of Ni-doped zinc oxide nanostructure: Comparatively more effective against Gram-negative bacteria including multi-drug resistant strains","volume":"10","author":"Naskar","year":"2020","journal-title":"RSC Adv."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"111400","DOI":"10.1016\/j.vacuum.2022.111400","article-title":"Enhanced ferro magnetism of (Cu, Fe\/Mn) dual doped ZnO nanoparticles and assessment of in-vitro cytotoxicity and antimicrobial activity for magnetically guided immunotherapy and hyperthermia applications","volume":"205","author":"Muthusamy","year":"2022","journal-title":"Vacuum"},{"key":"ref_32","unstructured":"Abd-Elsalam, K.A. (2021). Silver Nanomaterials for Agri-Food Applications Nanobiotechnology for Plant Protection, Elsevier."},{"key":"ref_33","first-page":"1","article-title":"Nanopart\u00edculas de plata: Un enfoque en aplicaciones biol\u00f3gicas","volume":"12","year":"2018","journal-title":"Mundo Nano Rev. Interdiscip. En. Nanociencias Nanotecnol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Giba\u0142a, A., \u017beliszewska, P., Gosiewski, T., Krawczyk, A., Duraczy\u0144ska, D., Szaleniec, J., Szaleniec, M., and O\u0107wieja, M. (2021). Antibacterial and Antifungal Properties of Silver Nanoparticles-Effect of a Surface-Stabilizing Agent. Biomolecules, 11.","DOI":"10.3390\/biom11101481"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"949","DOI":"10.15251\/DJNB.2022.173.949","article-title":"Hydrothermal synthesis, characterization and antimicrobial activity of Ag-modified ZnO nanoplates against pathogen","volume":"17","author":"Ullah","year":"2022","journal-title":"Dig. J. Nanomater. Biostruct."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00449-022-02815-8","article-title":"In Vitro anticancer and antibacterial performance of biosynthesized Ag and Ce co-doped ZnO NPs","volume":"46","author":"Alsamhary","year":"2023","journal-title":"Bioprocess. Biosyst. Eng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1186\/s11671-018-2643-x","article-title":"Influence of Mg Doping on ZnO Nanoparticles for Enhanced Photocatalytic Evaluation and Antibacterial Analysis","volume":"13","author":"Sadaiyandi","year":"2018","journal-title":"Nanoscale Res. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Cardoza-Contreras, M.N., S\u00e1nchez-Serrano, S., and Contreras, O.E. (2020). Highly Efficient photocatalytic and Antimicrobial AgGaCl Tri-Doped ZnO Nanorods for Water Treatment under Visible Light Irradiation. Catalysts, 10.","DOI":"10.3390\/catal10070752"},{"key":"ref_39","unstructured":"Ba\u00fa Betim Cazarin, C., Lemos Bicas, J., Maria Pastore, G., and Marostica, M.R. (2022). Bioactive Food Components Activity in Mechanistic Approach, Elsevier Academic Press."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10631","DOI":"10.1007\/s00253-020-10998-x","article-title":"Phytogenic products, used as alternatives to antibiotic growth promoters, modify the intestinal microbiota derived from a range of production systems: An in vitro model","volume":"104","author":"Bajagai","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"735944","DOI":"10.1016\/j.aquaculture.2020.735944","article-title":"Dietary menthol essential oil enhanced the growth performance, digestive enzyme activity, immune-related genes, and resistance against acute ammonia exposure in Nile tilapia (Oreochromis niloticus)","volume":"530","author":"Magouz","year":"2021","journal-title":"Aquaculture"},{"key":"ref_42","first-page":"175","article-title":"Effects of Eucalyptus globulus leaf extract on growth performance, feed utilization and blood biochemistry of Nile tilapia, Oreochromis niloticus","volume":"71","author":"Nurudeen","year":"2022","journal-title":"Veterinaria"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ibrahim, D., Shahin, S.E., Alqahtani, L.S., Hassan, Z., Althobaiti, F., Albogami, S., Soliman, M.M., El-Malt, R.M.S., Al-Harthi, H.F., and Alqadri, N. (2022). Exploring the Interactive Effects of Thymol and Thymoquinone: Moving towards an Enhanced Performance, Gross Margin, Immunity and Aeromonas sobria Resistance of Nile Tilapia (Oreochromis niloticus). Animals, 12.","DOI":"10.3390\/ani12213034"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.aquaculture.2018.01.036","article-title":"Effects of dietary limonene and thymol on the growth and nutritional physiology of Nile tilapia (Oreochromis niloticus)","volume":"488","author":"Aanyu","year":"2018","journal-title":"Aquaculture"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Nik Mohamad Nek Rahimi, N., Natrah, I., Loh, J.-Y., Ervin Ranzil, F.K., Gina, M., Lim, S.-H.E., Lai, K.-S., and Chong, C.-M. (2022). Phytocompounds as an Alternative Antimicrobial Approach in Aquaculture. Antibiotics, 11.","DOI":"10.3390\/antibiotics11040469"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"246","DOI":"10.31398\/tpjf\/27.2.2020-0006","article-title":"Phytogenics in Aquaculture: A Short Review of Their Effects on Gut Health and Microflora in Fish","volume":"27","author":"Caipang","year":"2020","journal-title":"Philipp. J. Fish."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3390\/pathogens4010137","article-title":"Phytogenic Compounds as Alternatives to In-Feed Antibiotics: Potentials and Challenges in Application","volume":"4","author":"Yang","year":"2015","journal-title":"Pathogens"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"46459","DOI":"10.1002\/app.46459","article-title":"The structural (FTIR, XRD, and XPS) and biological studies of thermosensitive chitosan chloride gels with \u03b2-glycerophosphate disodium","volume":"135","author":"Skwarczynska","year":"2018","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Silvestro, I., Francolini, I., Di Lisio, V., Martinelli, A., Pietrelli, L., Scotto d\u2019Abusco, A., Scoppio, A., and Piozzi, A. (2020). Preparation and Characterization of TPP-Chitosan Crosslinked Scaffolds for Tissue Engineering. Materials, 13.","DOI":"10.3390\/ma13163577"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.flm.2018.07.003","article-title":"Synthesis of chitosan nanoparticles by TPP and their potential mosquito larvicidal application","volume":"2","author":"Anand","year":"2018","journal-title":"Front. Lab. Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.ijbiomac.2012.08.032","article-title":"Chitosan\/TPP microparticles obtained by microemulsion method applied in controlled release of heparin","volume":"51","author":"Martins","year":"2012","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Kavi Rajan, R., Hussein, M.Z., Fakurazi, S., Yusoff, K., and Masarudin, M.J. (2019). Increased ROS Scavenging and Antioxidant Efficiency of Chlorogenic Acid Compound Delivered via a Chitosan Nanoparticulate System for Efficient In Vitro Visualization and Accumulation in Human Renal Adenocarcinoma Cells. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20194667"},{"key":"ref_53","first-page":"141","article-title":"Does the Use of Chitosan Contribute to Oxalate Kidney Stone Formation?","volume":"13","author":"Melo","year":"2015","journal-title":"Mar. Drugs"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Salehizadeh, H., Hekmatian, E., Sadeghi, M., and Kennedy, K. (2012). Synthesis and characterization of core-shell Fe3O4-gold-chitosan nanostructure. J. Nanobiotechnol., 10.","DOI":"10.1186\/1477-3155-10-3"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"20224","DOI":"10.1021\/acsomega.0c01903","article-title":"Extraction, Characterization, and Antimicrobial Activity of Chitosan from Horse Mussel Modiolus modiolus","volume":"5","author":"Varma","year":"2020","journal-title":"ACS Omega"},{"key":"ref_56","first-page":"419","article-title":"Effects of aluminum (Al) incorporation on structural, optical and thermal properties of ZnO nanoparticles","volume":"36","author":"Ahammed","year":"2018","journal-title":"Mater. Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1080\/1023666X.2014.902577","article-title":"Extraction and characterization of chitin and chitosan from Parapenaeus longirostris from moroccan local sources","volume":"19","author":"Dahmane","year":"2014","journal-title":"Int. J. Polym. Anal. Charact."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"8572","DOI":"10.1021\/acsomega.9b04421","article-title":"DFT-Calculated IR Spectrum Amide I, II, and III Band Contributions of N-Methylacetamide Fine Components","volume":"5","author":"Ji","year":"2020","journal-title":"ACS Omega"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fischetti, T., Celikkin, N., Contessi, N., Far\u00e8, S., and Swieszkowski, W. (2020). Tripolyphosphate-Crosslinked Chitosan\/Gelatin Biocomposite Ink for 3D Printing of Uniaxial Scaffolds. Front. Bioeng. Biotechnol., 8.","DOI":"10.3389\/fbioe.2020.00400"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/j.apsusc.2016.02.124","article-title":"Chemical modification of chitosan film via surface grafting of citric acid molecular to promote the biomineralization","volume":"370","author":"Liu","year":"2016","journal-title":"Appl. Surf. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1039\/C9GC00166B","article-title":"Functionalized chitosan adsorbents allow recovery of palladium and platinum from acidic aqueous solutions","volume":"21","author":"Mincke","year":"2019","journal-title":"Green Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2954","DOI":"10.1007\/s12221-021-0397-z","article-title":"Physicochemical Properties of TPP-Crosslinked Chitosan Nanoparticles as Potential Antibacterial Agents","volume":"22","author":"Khoerunnisa","year":"2021","journal-title":"Fibers Polym."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1208\/pt070250","article-title":"Studies on Effect of pH on Cross-linking of Chitosan with Sodium Tripolyphosphate: A Technical Note","volume":"7","author":"Bhumkar","year":"2006","journal-title":"AAPS PharmSciTech"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4589","DOI":"10.1016\/S0032-3861(99)00685-0","article-title":"Novel pH-sensitive chitosan hydrogels: Swelling behavior and states of water","volume":"41","author":"Qu","year":"2000","journal-title":"Polymer"},{"key":"ref_65","unstructured":"Millamena, O.M., Coloso, R.M., Pascual, F.P., and Iloilo, T. (2002). Nutrition in Tropical Aquaculture: Essentials of Fish Nutrition, Feeds, and Feeding of Tropical Aquatic Species, Aquaculture Department, Southeast Asian Fisheries Development Center. Available online: http:\/\/hdl.handle.net\/10862\/3316."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Gilannejad, N., Mart\u00ednez-Rodr\u00edguez, G., Y\u00fafera, M., and Moyano, F.J. (2018). Modelling digestive hydrolysis of nutrients in fish using factorial designs and desirability function. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0206556"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S1546-5098(10)03002-5","article-title":"2\u2014Feeding, digestion and absorption of nutrients","volume":"Volume 30","author":"Grosell","year":"2010","journal-title":"Fish Physiology"},{"key":"ref_68","unstructured":"Robert, J., Ouellette, J., and Rawn, D. (2015). Principles of Organic Chemistry, Elsevier."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/15\/20\/4105\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:07:44Z","timestamp":1760130464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/15\/20\/4105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,16]]},"references-count":68,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["polym15204105"],"URL":"https:\/\/doi.org\/10.3390\/polym15204105","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,16]]}}}