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Different approaches can be followed in order to achieve the bioconjugation of drugs and biological compounds onto polymer surfaces. In this study, we focused on the immobilization of an amoxicillin antibiotic onto the surface of poly-L-lactic acid (PLLA) using a copper-free amino-yne click reaction. The utilization of this reaction allowed for a selective and efficient bioconjugation of the amoxicillin moiety onto the PLLA surface, avoiding copper-related concerns and ensuring biocompatibility. The process involved sequential steps that included surface activation via alkaline hydrolysis followed by an amidation reaction with ethylendiamine, functionalization with propiolic groups, and subsequent conjugation with amoxicillin via a click chemistry approach. Previous amoxicillin immobilization using tryptophan and fluorescent amino acid conjugation was carried out in order to determine the efficacy of the proposed methodology. Characterization techniques such as X-ray photoelectron spectroscopy (XPS), Attenuated Total Reflection (ATR)\u2013Fourier Transform Infrared (FTIR) spectroscopy, surface imaging, water contact angle determination, and spectroscopic analysis confirmed the successful immobilization of both tryptophan and amoxicillin while maintaining the integrity of the PLLA surface. This tailored modification not only exhibited a novel method for surface functionalization but also opens avenues for developing antimicrobial biomaterials with improved drug-loading capacity.<\/jats:p>","DOI":"10.3390\/polym16020246","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T11:15:01Z","timestamp":1705317301000},"page":"246","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Catalyst-Free Amino-Yne Click Reaction: An Efficient Way for Immobilizing Amoxicillin onto Polymeric Surfaces"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3576-1526","authenticated-orcid":false,"given":"Julia","family":"S\u00e1nchez-Bod\u00f3n","sequence":"first","affiliation":[{"name":"Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Diaz-Galbarriatu","sequence":"additional","affiliation":[{"name":"Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-3644","authenticated-orcid":false,"given":"Rebeca","family":"Sola-Llano","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2045-008X","authenticated-orcid":false,"given":"Leire","family":"Ruiz-Rubio","sequence":"additional","affiliation":[{"name":"Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"},{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU, Science Park, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Vilas-Vilela","sequence":"additional","affiliation":[{"name":"Macromolecular Chemistry Group (LABQUIMAC), Department of Physical Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"},{"name":"BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV\/EHU, Science Park, 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8714-9120","authenticated-orcid":false,"given":"Isabel","family":"Moreno-Benitez","sequence":"additional","affiliation":[{"name":"Macromolecular Chemistry Group (LABQUIMAC), Department of Organic and Inorganic Chemistry, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Taib, N.A.A.B., Rahman, M.R., Huda, D., Kuok, K.K., Hamdan, S., Bakri, M.K.B., Julaihi, M.R.M.B., and Khan, A. 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