{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T03:22:32Z","timestamp":1792725752760,"version":"4.5.0"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T00:00:00Z","timestamp":1713312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Nowadays, increased food safety and decreased food waste are two of the major global interests. Self-healable active packaging materials are an attractive option to achieve such targets. This property is critical for the hygiene and the consumption appropriateness of the food. Polylactic acid is a very promising polymeric matrix that potentially could replace the widely used low-density polyethylene due to its biobased origin and its easy biodegradable nature. The main drawback of this polymeric matrix is its brittle, fragile nature. On the other hand, tetraethyl citrate is a biobased approved food additive which became an attractive option as a plasticizer for industries seeking alternative materials to replace the traditional petrochemically derived compounds. A novel biobased film exhibiting self-healing behavior suitable for food-active packaging was developed during this study. Polylactic acid\u2019s brittleness was reduced drastically by incorporating tetraethyl citrate, and a random cut on the original self-repairing film was fully healed after 120 s. The optimum concentration of tetraethyl citrate in the polylactic acid was around 15% v\/w with a water\/oxygen barrier close to the relevant of polylactic acid and low migration. According to the EC50 parameter, the antioxidant activity was 300% higher than the relevant of pure polylactic acid, while according to the thiobarbituric acid and heme iron parameters, the film resisted lipid oxidation and deterioration. Finally, the total viable count parameter indicates the strong antimicrobial activity of this sample.<\/jats:p>","DOI":"10.3390\/polym16081130","type":"journal-article","created":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T10:52:37Z","timestamp":1713351157000},"page":"1130","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["\u039dovel Polylactic Acid\/Tetraethyl Citrate Self-Healable Active Packaging Films Applied to Pork Fillets\u2019 Shelf-Life Extension"],"prefix":"10.3390","volume":"16","author":[{"given":"Vassilios K.","family":"Karabagias","sequence":"first","affiliation":[{"name":"Department of Food Science and Technology, University of Patras, 30100 Agrinio, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3585-9045","authenticated-orcid":false,"given":"Aris E.","family":"Giannakas","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Patras, 30100 Agrinio, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7784-6424","authenticated-orcid":false,"given":"Nikolaos D.","family":"Andritsos","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Patras, 30100 Agrinio, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2209-8355","authenticated-orcid":false,"given":"Dimitrios","family":"Moschovas","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2773-0703","authenticated-orcid":false,"given":"Andreas","family":"Karydis-Messinis","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0918-7759","authenticated-orcid":false,"given":"Areti","family":"Leontiou","sequence":"additional","affiliation":[{"name":"Department of Food Science and Technology, University of Patras, 30100 Agrinio, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6203-9942","authenticated-orcid":false,"given":"Apostolos","family":"Avgeropoulos","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8107-0075","authenticated-orcid":false,"given":"Nikolaos E.","family":"Zafeiropoulos","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3450-5969","authenticated-orcid":false,"given":"Charalampos","family":"Proestos","sequence":"additional","affiliation":[{"name":"Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens Zografou, 15771 Athens, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9475-9354","authenticated-orcid":false,"given":"Constantinos E.","family":"Salmas","sequence":"additional","affiliation":[{"name":"Department of Material Science and Engineering, University of Ioannina, 45110 Ioannina, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"136283","DOI":"10.1016\/j.jclepro.2023.136283","article-title":"A Circular Economy Framework for Seafood Waste Valorisation to Meet Challenges and Opportunities for Intensive Production and Sustainability","volume":"392","author":"Cooney","year":"2023","journal-title":"J. Clean. Prod."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Esposito, B., Sessa, M.R., Sica, D., and Malandrino, O. (2020). Towards Circular Economy in the Agri-Food Sector. A Systematic Literature Review. Sustainability, 12.","DOI":"10.3390\/su12187401"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Hamam, M., Chinnici, G., Di Vita, G., Pappalardo, G., Pecorino, B., Maesano, G., and D\u2019Amico, M. (2021). Circular Economy Models in Agro-Food Systems: A Review. Sustainability, 13.","DOI":"10.3390\/su13063453"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nemat, B., Razzaghi, M., Bolton, K., and Rousta, K. (2019). The Role of Food Packaging Design in Consumer Recycling Behavior\u2014A Literature Review. Sustainability, 11.","DOI":"10.3390\/su11164350"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"de Sousa, M.S., Schlogl, A.E., Estanislau, F.R., Souza, V.G.L., dos Reis Coimbra, J.S., and Santos, I.J.B. (2023). Nanotechnology in Packaging for Food Industry: Past, Present, and Future. Coatings, 13.","DOI":"10.3390\/coatings13081411"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"126733","DOI":"10.1016\/j.jclepro.2021.126733","article-title":"Food Packaging and Sustainability\u2013Consumer Perception vs. Correlated Scientific Facts: A Review","volume":"298","author":"Otto","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.1007\/s11947-023-03048-7","article-title":"Carrageenan\/Alginate-Based Functional Films Incorporated with Allium Sativum Carbon Dots for UV-Barrier Food Packaging","volume":"16","author":"Khan","year":"2023","journal-title":"Food Bioprocess Technol."},{"key":"ref_8","first-page":"55","article-title":"Advances in Food Packaging Technology\u2014A Review","volume":"6","author":"Abdullahi","year":"2018","journal-title":"J. Postharvest Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.foodhyd.2016.09.009","article-title":"Polysaccharide-Based Films and Coatings for Food Packaging: A Review","volume":"68","author":"Velazquez","year":"2017","journal-title":"Food Hydrocoll."},{"key":"ref_10","unstructured":"Giannakas, A.E. (2023). Natural Materials for Food Packaging Application, John Wiley & Sons, Ltd."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Acquavia, M.A., Pascale, R., Martelli, G., Bondoni, M., and Bianco, G. (2021). Natural Polymeric Materials: A Solution to Plastic Pollution from the Agro-Food Sector. Polymers, 13.","DOI":"10.3390\/polym13010158"},{"key":"ref_12","unstructured":"European Bioplastics e.V. (2022, March 12). Available online: https:\/\/www.european-bioplastics.org\/."},{"key":"ref_13","unstructured":"(2023, August 16). Handbook of Bioplastics and Biocomposites Engineering Applications|Wiley Online Books. Available online: https:\/\/onlinelibrary.wiley.com\/doi\/book\/10.1002\/9781118203699."},{"key":"ref_14","unstructured":"(2023, August 16). Biobased Materials in Food Packaging Applications\u2014Handbook of Bioplastics and Biocomposites Engineering Applications\u2014Wiley Online Library. Available online: https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/9781118203699.ch5."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.addr.2016.06.012","article-title":"Physical and Mechanical Properties of PLA, and Their Functions in Widespread Applications\u2014A Comprehensive Review","volume":"107","author":"Farah","year":"2016","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1117749","DOI":"10.1080\/23311932.2015.1117749","article-title":"Bioplastics and Food Packaging: A Review","volume":"1","author":"Jabeen","year":"2015","journal-title":"Cogent Food Agric."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"127111","DOI":"10.1016\/j.jclepro.2021.127111","article-title":"Sustainability Governance and Contested Plastic Food Packaging\u2013An Integrative Review","volume":"306","year":"2021","journal-title":"J. Clean. Prod."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Siracusa, V., and Blanco, I. (2020). Bio-Polyethylene (Bio-PE), Bio-Polypropylene (Bio-PP) and Bio-Poly(Ethylene Terephthalate) (Bio-PET): Recent Developments in Bio-Based Polymers Analogous to Petroleum-Derived Ones for Packaging and Engineering Applications. Polymers, 12.","DOI":"10.3390\/polym12081641"},{"key":"ref_19","unstructured":"(2023, August 16). Polylactic Acid (PLA) Foams for Packaging Applications\u2014Handbook of Bioplastics and Biocomposites Engineering Applications\u2014Wiley Online Library. Available online: https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/9781118203699.ch6."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1002\/adv.21864","article-title":"Biosourced Blends Based on Poly (Lactic Acid) and Polyamide 11: Structure\u2013Properties Relationships and Enhancement of Film Blowing Processability","volume":"37","author":"Walha","year":"2018","journal-title":"Adv. Polym. Technol."},{"key":"ref_21","unstructured":"(2023, August 16). Eco-Friendly Plasticizers for PLA Films\u2014Italian Food Tech 2019. Available online: https:\/\/www.italianfoodtech.com\/eco-friendly-plasticizers-for-pla-films."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"16024","DOI":"10.3390\/molecules191016024","article-title":"Epoxidized Vegetable Oils Plasticized Poly(Lactic Acid) Biocomposites: Mechanical, Thermal and Morphology Properties","volume":"19","author":"Chieng","year":"2014","journal-title":"Molecules"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"022062","DOI":"10.1088\/1755-1315\/108\/2\/022062","article-title":"Plasticization of Poly (Lactic) Acid Film as a Potential Coating Material","volume":"108","author":"Yang","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1021\/acsomega.8b02880","article-title":"Influence of Cardanol Oil on the Properties of Poly(Lactic Acid) Films Produced by Melt Extrusion","volume":"4","author":"Mele","year":"2019","journal-title":"ACS Omega"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"11643","DOI":"10.1039\/C7RA13448G","article-title":"An Environmentally Sustainable Plasticizer Toughened Polylactide","volume":"8","author":"Kang","year":"2018","journal-title":"RSC Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1515\/polyeng-2015-0140","article-title":"Plasticizing Effects of Citrate Esters on Properties of Poly(Lactic Acid)","volume":"36","author":"Maiza","year":"2016","journal-title":"J. Polym. Eng."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"50302","DOI":"10.1002\/app.50302","article-title":"Polylactide Films Produced with Bixin and Acetyl Tributyl Citrate: Functional Properties for Active Packaging","volume":"138","author":"Stoll","year":"2021","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_28","unstructured":"(2023, August 16). SciELO\u2014Brazil\u2014Biobased Additive Plasticizing Polylactic Acid (PLA) Biobased Additive Plasticizing Polylactic Acid (PLA). Available online: https:\/\/www.scielo.br\/j\/po\/a\/HcnbBHn63pPbkLwmKtmfwpb\/."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Herrera, N., Singh, A.A., Salaberria, A.M., Labidi, J., Mathew, A.P., and Oksman, K. (2017). Triethyl Citrate (TEC) as a Dispersing Aid in Polylactic Acid\/Chitin Nanocomposites Prepared via Liquid-Assisted Extrusion. Polymers, 9, Available online: https:\/\/www.mdpi.com\/2073-4360\/9\/9\/406.","DOI":"10.3390\/polym9090406"},{"key":"ref_30","unstructured":"(2024, January 25). PubChem Triethyl Citrate, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/6506."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Salmas, C.E., Giannakas, A.E., Karabagias, V.K., Moschovas, D., Karabagias, I.K., Gioti, C., Georgopoulos, S., Leontiou, A., Kehayias, G., and Avgeropoulos, A. (2023). Development and Evaluation of a Novel-Thymol@Natural-Zeolite\/Low-Density-Polyethylene Active Packaging Film: Applications for Pork Fillets Preservation. Antioxidants, 12.","DOI":"10.3390\/antiox12020523"},{"key":"ref_32","first-page":"100338","article-title":"Development, Physicochemical Characterization and in Vitro Evaluation of Chitosan-Fish Gelatin-Glycerol Hydrogel Membranes for Wound Treatment Applications","volume":"6","author":"Moschovas","year":"2023","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Fellows, P.J. (2009). Food Processing Technology, Woodhead Publishing. [3rd ed.].","DOI":"10.1533\/9781845696344"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Giannakas, A.E., Salmas, C.E., Leontiou, A., Moschovas, D., Baikousi, M., Kollia, E., Tsigkou, V., Karakassides, A., Avgeropoulos, A., and Proestos, C. (2022). Performance of Thyme Oil@Na-Montmorillonite and Thyme Oil@Organo-Modified Montmorillonite Nanostructures on the Development of Melt-Extruded Poly-L-Lactic Acid Antioxidant Active Packaging Films. Molecules, 27.","DOI":"10.3390\/molecules27041231"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Giannakas, A.E., Salmas, C.E., Moschovas, D., Baikousi, M., Kollia, E., Tsigkou, V., Karakassides, A., Leontiou, A., Kehayias, G., and Avgeropoulos, A. (2022). Nanocomposite Film Development Based on Chitosan\/Polyvinyl Alcohol Using ZnO@Montmorillonite and ZnO@Halloysite Hybrid Nanostructures for Active Food Packaging Applications. Nanomaterials, 12.","DOI":"10.3390\/nano12111843"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.foodchem.2011.07.127","article-title":"Estimation of Antiradical Properties of Antioxidants Using DPPH Assay: A Critical Review and Results","volume":"130","author":"Mishra","year":"2012","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0023-6438(95)80008-5","article-title":"Use of a Free Radical Method to Evaluate Antioxidant Activity","volume":"28","author":"Cuvelier","year":"1995","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e54465","DOI":"10.1002\/app.54465","article-title":"Atomic Layer Deposition of ZnO on PLA\/TiO2 Bionanocomposites: Evaluation of Surface Chemistry and Physical Properties toward Food Packaging Applications","volume":"140","author":"Barmpaki","year":"2023","journal-title":"J. Appl. Polym. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/BF02630824","article-title":"A Distillation Method for the Quantitative Determination of Malonaldehyde in Rancid Foods","volume":"37","author":"Tarladgis","year":"1960","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1021\/jf960054l","article-title":"Heme and Total Iron in Ready-to-Eat Chicken","volume":"45","author":"Clark","year":"1997","journal-title":"J. Agric. Food Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1007\/s13197-020-04601-4","article-title":"Shelf Life Evaluation of Fresh Chicken Burgers Based on the Combination of Chitosan Dip and Vacuum Packaging under Refrigerated Storage","volume":"58","author":"Assanti","year":"2021","journal-title":"J. Food Sci. Technol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"100751","DOI":"10.1016\/j.fpsl.2021.100751","article-title":"In Vitro Antibacterial Activity of Gelatin-Nanochitosan Films Incorporated with Zataria Multiflora Boiss Essential Oil and Its Influence on Microbial, Chemical, and Sensorial Properties of Chicken Breast Meat during Refrigerated Storage","volume":"30","author":"Hematizad","year":"2021","journal-title":"Food Packag. Shelf Life"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Kobayashi, S., and M\u00fcllen, K. (2021). Encyclopedia of Polymeric Nanomaterials, Springer.","DOI":"10.1007\/978-3-642-36199-9"},{"key":"ref_44","unstructured":"(2023, October 26). Triethyl Citrate(77-93-0) IR2 Spectrum. Available online: https:\/\/www.chemicalbook.com\/SpectrumEN_77-93-0_IR2.htm."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/S1881-8366(11)80018-2","article-title":"Water Vapor Transmission Rate of Biomass Based Film Materials","volume":"4","author":"Basha","year":"2011","journal-title":"Eng. Agric. Environ. Food"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Giannakas, A.E., Salmas, C.E., Moschovas, D., Karabagias, V.K., Karabagias, I.K., Baikousi, M., Georgopoulos, S., Leontiou, A., Katerinopoulou, K., and Zafeiropoulos, N.E. (2023). Development, Characterization, and Evaluation as Food Active Packaging of Low-Density-Polyethylene-Based Films Incorporated with Rich in Thymol Halloysite Nanohybrid for Fresh \u201cScaloppini\u201d Type Pork Meat Fillets Preservation. Polymers, 15.","DOI":"10.3390\/polym15020282"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0309-1740(87)90078-7","article-title":"Micro-Organisms in Food\u20142. Sampling for Microbiological Analysis: Principles and Specific Applications: ICMSF, Blackwell Scientific Publications, Oxford, 1986. 310 Pp. Price: \u00a319\u00b750 (Cloth)","volume":"19","author":"Stewart","year":"1987","journal-title":"Meat Sci."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/16\/8\/1130\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:29:29Z","timestamp":1760106569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/16\/8\/1130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,17]]},"references-count":47,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["polym16081130"],"URL":"https:\/\/doi.org\/10.3390\/polym16081130","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,17]]}}}