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Autogenic and allogenic grafts are the gold standard used to treat and regenerate damaged or defective bone segments. However, these materials do not possess the antimicrobial properties necessary to inhibit the invasion of the numerous deleterious pathogens present in the oral microbiota. In the present study, poly(\u03b5-caprolactone) (PCL), nano-hydroxyapatite (nHAp), and a commercial extract of Humulus lupulus L. (hops) were electrospun into polymeric matrices to assess their potential for drug delivery and bone regeneration. The fabricated matrices were analyzed using scanning electron microscopy (SEM), tensile analysis, thermogravimetric analysis (TGA), FTIR assay, and in vitro hydrolytic degradation. The antimicrobial properties were evaluated against the oral pathogens Streptococcus mutans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. The cytocompatibility was proved using the MTT assay. SEM analysis established the nanostructured matrices present in the three-dimensional interconnected network. The present research provides new information about the interaction of natural compounds with ceramic and polymeric biomaterials. The hop extract and other natural or synthetic medicinal agents can be effectively loaded into PCL fibers and have the potential to be used in oral applications.<\/jats:p>","DOI":"10.3390\/polym16091258","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T08:02:22Z","timestamp":1714723342000},"page":"1258","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Nanofibrous \u03b5-Polycaprolactone Matrices Containing Nano-Hydroxyapatite and Humulus lupulus L. Extract: Physicochemical and Biological Characterization for Oral Applications"],"prefix":"10.3390","volume":"16","author":[{"given":"Jaime","family":"Villanueva-Lumbreras","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Ave. Pedro de Alba S\/N, San Nicol\u00e1s de los Garza 66455, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2289-4239","authenticated-orcid":false,"given":"Ciro","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Tecnologico de Monterrey, Escuela de Ingenier\u00eda y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico"},{"name":"Laboratorio Nacional de Manufactura Aditiva y Digital (MADIT), Apodaca 66629, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7395-5754","authenticated-orcid":false,"given":"Mar\u00eda Rosa","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Instituto de Ciencia y Tecnolog\u00eda de Pol\u00edmeros (ICTP-CSIC), 28006 Madrid, Spain"},{"name":"Networking Biomedical Research Centre in Bioengineering, Biomaterials and Nanomedicine, CIBER.BBN, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamlet","family":"Avil\u00e9s-Arnaut","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Ave. Pedro de Alba S\/N, San Nicol\u00e1s de los Garza 66455, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2536-3097","authenticated-orcid":false,"given":"Geoffrey A.","family":"Cordell","sequence":"additional","affiliation":[{"name":"Natural Products Inc., Evanston, IL 60201, USA"},{"name":"College of Pharmacy, University of Florida, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0299-4753","authenticated-orcid":false,"given":"Aida","family":"Rodriguez-Garcia","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Ave. Pedro de Alba S\/N, San Nicol\u00e1s de los Garza 66455, NL, Mexico"},{"name":"Tecnologico de Monterrey, Escuela de Ingenier\u00eda y Ciencias, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,30]]},"reference":[{"key":"ref_1","first-page":"38","article-title":"Nanobiotechnology\u2014Drug delivery and tissue engineering","volume":"102","author":"Khademhosseini","year":"2006","journal-title":"Chem. Eng. Prog."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Carvalho, M.S., Cabral, J., da Silva, C.L., and Vashishth, D. (2021). Bone matrix non-collagenous proteins in tissue engineering: Creating new bone by mimicking the extracellular matrix. Polymers, 13.","DOI":"10.3390\/polym13071095"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2025","DOI":"10.1039\/C8BM00518D","article-title":"Biocompatibility of polymer-based biomaterials and medical devices\u2014Regulations, in vitro screening and risk-management","volume":"6","author":"Bernard","year":"2018","journal-title":"Biomater. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/wrr.13126","article-title":"Nanofibrous polycaprolactone\/gelatin scaffold containing gold nanoparticles: Physicochemical and biological characterization for wound healing","volume":"31","author":"Cheraghali","year":"2023","journal-title":"Wound Repair Regen."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125473","DOI":"10.1016\/j.polymer.2022.125473","article-title":"Tuning polycaprolactone degradation for long acting implantables","volume":"262","author":"Lykins","year":"2022","journal-title":"Polymer"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Rahimkhoei, V., Padervand, M., Hedayat, M., Seidi, F., Dawi, E.A., and Akbari, A. (2023). Biomedical applications of electrospun polycaprolactone-based carbohydrate polymers: A review. Int. J. Biol. Macromol., 253.","DOI":"10.1016\/j.ijbiomac.2023.126642"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"6477","DOI":"10.2147\/IJN.S298936","article-title":"Nanomaterials\u2014Upconverted hydroxyapatite for bone tissue engineering and a platform for drug delivery","volume":"16","author":"Halim","year":"2021","journal-title":"Int. J. Nanomed."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1080\/21691401.2021.2016785","article-title":"Nano-hydroxyapatite as a delivery system: Overview and advancements","volume":"49","author":"Munir","year":"2021","journal-title":"Artif. Cells Nanomed. Biotechnol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"108790","DOI":"10.1016\/j.compositesb.2021.108790","article-title":"Recent advances in biomedical engineering of nano-hydroxyapatite including dentistry, cancer treatment and bone repair","volume":"215","author":"Du","year":"2021","journal-title":"Compos. B Eng."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Haider, M.K., Kharaghani, D., Sun, L., Ullah, S., Sarwar, M.N., Ullah, A., Khatri, M., Yoshiko, Y., Gopiraman, M., and Kim, I.S. (2023). Synthesized bioactive lignin nanoparticles\/polycaprolactone nanofibers: A novel nanobiocomposite for bone tissue engineering. Biomater. Adv., 144.","DOI":"10.1016\/j.bioadv.2022.213203"},{"key":"ref_11","first-page":"21","article-title":"Decoding bone-inspired and cell-instructive cues of scaffolds for bone tissue engineering","volume":"5","author":"Hussain","year":"2023","journal-title":"Eng. Regen."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.colsurfb.2018.11.003","article-title":"Magnetic bioinspired micro\/nanostructured composite scaffold for bone regeneration","volume":"174","author":"Zhao","year":"2019","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"116956","DOI":"10.1016\/j.bone.2023.116956","article-title":"Nano-hydroxyapatite structures for bone regenerative medicine: Cell-material interaction","volume":"179","author":"Hoveidaei","year":"2023","journal-title":"Bone"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Awad, H.A., O\u2019Keefe, R.J., and Mao, J.J. (2020). Principles of Tissue Engineering, Elsevier.","DOI":"10.1016\/B978-0-12-818422-6.00083-6"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12221","article-title":"Periodontitis: Facts, fallacies and the future","volume":"75","author":"Slots","year":"2017","journal-title":"Periodontol. 2000"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/mr.44.2.331-384.1980","article-title":"Biology, immunology, and cariogenicity of Streptococcus mutans","volume":"44","author":"Hamada","year":"1980","journal-title":"Microbiol. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"125542","DOI":"10.1016\/j.cej.2020.125542","article-title":"Hierarchically multi-functionalized graded membrane with enhanced bone regeneration and self-defensive antibacterial characteristics for guided bone regeneration","volume":"398","author":"He","year":"2020","journal-title":"Chem. Eng. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"134855","DOI":"10.1016\/j.cej.2022.134855","article-title":"Repair of infected bone defect with clindamycin-tetrahedral DNA nanostructure complex-loaded 3D bioprinted hybrid scaffold","volume":"435","author":"Li","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2100749","DOI":"10.1002\/advs.202100749","article-title":"Plant natural flavonoids against multidrug resistant pathogens","volume":"8","author":"Song","year":"2021","journal-title":"Adv. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105035","DOI":"10.1016\/j.jff.2022.105035","article-title":"Hops components and oral health","volume":"92","author":"Abiko","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"134959","DOI":"10.1016\/j.foodchem.2022.134959","article-title":"Humulus lupulus L. a potential precursor to human health: High hops craft beer","volume":"405","year":"2023","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"499","DOI":"10.4067\/S0718-58392023000400499","article-title":"Antimicrobial activity of compounds from hop (Humulus lupulus L.) following supercritical fluid extraction: An overview","volume":"83","author":"Betancur","year":"2023","journal-title":"Chil. J. Agric. Res."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Khaliullina, A., Kolesnikova, A., Khairullina, L., Morgatskaya, O., Shakirova, D., Patov, S., Nekrasova, P., Bogachev, M., Kurkin, V., and Trizna, E. (2024). The antimicrobial potential of the hop (Humulus lupulus L.) extract against Staphylococcus aureus and oral Streptococci. Pharmaceuticals, 17.","DOI":"10.3390\/ph17020162"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1021\/acs.chemrestox.8b00345","article-title":"The multiple biological targets of hops and bioactive compounds","volume":"32","author":"Bolton","year":"2019","journal-title":"Chem. Res. Toxicol."},{"key":"ref_25","first-page":"271","article-title":"Effect of xanthohumol on Porphyromonas gingivalis","volume":"62","author":"Hiraki","year":"2019","journal-title":"Jpn. J. Conserv. Dent."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Harish, V., Haque, E., \u015amiech, M., Taniguchi, H., Jamieson, S., Tewari, D., and Bishayee, A. (2021). Xanthohumol for human malignancies: Chemistry, pharmacokinetics and molecular targets. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094478"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.bbrc.2011.04.113","article-title":"Xanthohumol from the hop plant stimulates osteoblast differentiation by RUNX2 activation","volume":"409","author":"Jeong","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1002\/pat.5884","article-title":"Electrospun nanofibers for drug delivery applications: Methods and mechanism","volume":"34","author":"Thamer","year":"2023","journal-title":"Polym. Adv. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Liu, H., Bai, Y., Huang, C., Wang, Y., Ji, Y., Du, Y., and Bligh, S.W.A. (2023). Recent progress of electrospun herbal medicine nanofibers. Biomolecules, 13.","DOI":"10.3390\/biom13010184"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"110047","DOI":"10.1016\/j.matdes.2021.110047","article-title":"Electrospinning and 3D printed hybrid bi-layer scaffold for guided bone regeneration","volume":"210","author":"Liu","year":"2021","journal-title":"Mater. Des."},{"key":"ref_31","unstructured":"(2010). Biological Evaluation of Medical Devices\u2014Part 13: Identification and Quantification of Degradation Products from Polymeric Medical Devices (Standard No. ISO 10993-13)."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1093\/ajcp\/45.4_ts.493","article-title":"Antibiotic susceptibility testing by a standardized single disc method","volume":"45","author":"Bauer","year":"1966","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_33","unstructured":"(1993). Biological Evaluation of Medical Devices. Part 5: Tests for Cytotoxicity: In Vitro Methods (Standard No. ISO 10993-5)."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"125093","DOI":"10.1016\/j.ijbiomac.2023.125093","article-title":"A bilayer biocompatible polycaprolactone\/zinc oxide\/Capparis spinosa L. ethyl acetate extract\/polylactic acid nanofibrous composite scaffold for novel wound dressing applications","volume":"242","author":"Zhu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Irani, M., Abadi, P.G., Ahmadian-Attari, M.M., Rezaee, A., Kordbacheh, H., and Goleij, P. (2024). In vitro and in vivo studies of Dragon\u2019s blood plant (D. cinnabari)-loaded electrospun chitosan\/PCL nanofibers: Cytotoxicity, antibacterial, and wound healing activities. Int. J. Biol. Macromol., 257.","DOI":"10.1016\/j.ijbiomac.2023.128634"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"102315","DOI":"10.1016\/j.cis.2020.102315","article-title":"The impact of relative humidity on electrospun polymer fibers: From structural changes to fiber morphology","volume":"286","author":"Szewczyk","year":"2020","journal-title":"Adv. Colloid Interfac. Sci."},{"key":"ref_37","first-page":"58","article-title":"Electrospinning: Processes, structures, and materials","volume":"4","author":"Rodrigue","year":"2024","journal-title":"Macromolecules"},{"key":"ref_38","unstructured":"Chong, L.H., Hassan, M.I., and Sultana, N. (June, January 31). Electrospun polycaprolactone (PCL) and PCL\/nano-hydroxyapatite (PCL\/nHA)-based nanofibers for bone tissue engineering application. Proceedings of the IEEE 10th Asian Control Conference (ASCC), Sabah, Malaysia."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s13205-017-0889-0","article-title":"Characterization, drug loading and antibacterial activity of nanohydroxyapatite\/polycaprolactone (nHA\/PCL) electrospun membrane","volume":"7","author":"Hassan","year":"2017","journal-title":"3 Biotech."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Fortun\u0103, M.E., Ungureanu, E., Rotaru, R., Bargan, A., Ungureanu, O.C., Brezuleanu, C.O., and Harabagiu, V. (2023). Synthesis and properties of modified biodegradable polymers based on caprolactone. Polymers, 15.","DOI":"10.3390\/polym15244731"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Emadi, H., Karevan, M., Masoudi Rad, M., Sadeghzade, S., Pahlevanzadeh, F., Khodaei, M., Khayatzadeh, S., and Lotfian, S. (2023). Bioactive and biodegradable polycaprolactone-based nanocomposite for bone repair applications. Polymers, 15.","DOI":"10.3390\/polym15173617"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"105196","DOI":"10.1016\/j.mtcomm.2022.105196","article-title":"Biomechanical properties of a customizable TPU\/PCL blended esophageal stent fabricated by 3D printing","volume":"34","author":"Yu","year":"2023","journal-title":"Mater. Today Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1080\/03602550801897695","article-title":"Thermal stabilities and the thermal degradation kinetics of poly(\u03b5-caprolactone)","volume":"47","author":"Su","year":"2008","journal-title":"Polymer-Plast. Technol. Eng."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Motloung, M.P., Mofokeng, T.G., and Ray, S.S. (2021). Viscoelastic, thermal, and mechanical properties of melt-processed poly(\u03b5-caprolactone) (PCL)\/hydroxyapatite (HAP) composites. Materials, 15.","DOI":"10.3390\/ma15010104"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1002\/jbm.b.34761","article-title":"3D-printed cryomilled poly(\u03b5-caprolactone)\/graphene composite scaffolds for bone tissue regeneration","volume":"109","author":"Dias","year":"2021","journal-title":"J. Biomed. Mater. Res. B Appl. Biomater."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Loyo, C., Cordoba, A., Palza, H., Canales, D., Melo, F., Vivanco, J.F., Vallejos, R., Mill\u00e1n, C., Corrales, T., and Zapata, P.A. (2023). Effect of Gelatin Coating and GO incorporation on the properties and degradability of electrospun PCL scaffolds for bone tissue regeneration. Polymers, 16.","DOI":"10.3390\/polym16010129"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e1130","DOI":"10.1002\/jsp2.1130","article-title":"Electrospun biodegradable poly(\u03b5-caprolactone) membranes for annulus fibrosus repair: Long-term material stability and mechanical competence","volume":"4","author":"Alexeev","year":"2021","journal-title":"JOR Spine"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Swetha, S., Balagangadharan, K., Lavanya, K., and Selvamurugan, N. (2021). Three-dimensional-poly(lactic acid) scaffolds coated with gelatin\/magnesium-doped nano-hydroxyapatite for bone tissue engineering. Biotechnol. J., 16.","DOI":"10.1002\/biot.202100282"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.foodchem.2014.02.005","article-title":"Characteristics of compounds in hops using cyclic voltammetry, UV\u2013VIS, FTIR and GC\u2013MS analysis","volume":"156","author":"Masek","year":"2014","journal-title":"Food Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3296","DOI":"10.1002\/pat.6140","article-title":"Polycaprolactone and its derivatives for drug delivery","volume":"34","author":"Pawar","year":"2023","journal-title":"Polym. Adv. Technol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"9434","DOI":"10.1038\/s41598-023-36563-w","article-title":"Surface modification of polycaprolactone nanofibers through hydrolysis and aminolysis: A comparative study on structural characteristics, mechanical properties, and cellular performance","volume":"13","author":"Yaseri","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2100153","DOI":"10.1002\/adem.202100153","article-title":"Mechanical properties of electrospun fibers\u2014A critical review","volume":"23","author":"Rashid","year":"2021","journal-title":"Adv. Eng. Mater."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/j.compositesb.2008.01.002","article-title":"Effects of particle size, particle\/matrix interface adhesion and particle loading on mechanical properties of particulate\u2013polymer composites","volume":"39","author":"Fu","year":"2008","journal-title":"Composites Part B Eng."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.actbio.2011.08.015","article-title":"Mechanical testing of electrospun PCL fibers","volume":"8","author":"Croisier","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"105773","DOI":"10.1016\/j.mtcomm.2023.105773","article-title":"The effect of molecular weight and fiber diameter on the mechanical properties of single, electrospun PCL nanofibers","volume":"35","author":"Alharbi","year":"2023","journal-title":"Mater. Today Commun."},{"key":"ref_56","first-page":"09673911231151506","article-title":"Fabrication of chitosan\/polycaprolactone\/Myrtus communis L. extract nanofibrous mats with enhanced antibacterial activities","volume":"31","author":"Ramazani","year":"2023","journal-title":"Polym. Polym. Compos."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Moraczewski, K., Stepczy\u0144ska, M., Malinowski, R., Karasiewicz, T., Jagodzi\u0144ski, B., and Rytlewski, P. (2023). Modification of polycaprolactone with plant extracts to improve the aging resistance. Materials, 16.","DOI":"10.3390\/ma16145154"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s13726-018-0674-x","article-title":"Preparation and characterization of Calendula officinalis-loaded PCL\/gum Arabic nanocomposite scaffolds for wound healing applications","volume":"28","author":"Mokhtari","year":"2019","journal-title":"Iran. Polym. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1663\/0013-0001(2003)057[0118:IOSMAO]2.0.CO;2","article-title":"Inhibition of Streptococcus mutans and other oral Streptococci by hop (Humulus lupulus L.) constituents","volume":"57","author":"Bhattacharya","year":"2003","journal-title":"Econ. Bot."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"59","DOI":"10.4315\/0362-028X-57.1.59","article-title":"Antimicrobial activity of hop resins","volume":"57","author":"Haas","year":"1994","journal-title":"J. Food Prot."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Teuber, M. (1970). Low antibiotic potency of isohumulone. Appl. Microbiol., 19.","DOI":"10.1128\/am.19.5.871-871.1970"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Klimek, K., Ty\u015bkiewicz, K., Miazga-Karska, M., D\u0119bczak, A., R\u00f3j, E., and Ginalska, G. (2021). Bioactive compounds obtained from Polish \u201cMarynka\u201d hop variety using efficient two-step supercritical fluid extraction and comparison of their antibacterial, cytotoxic, and anti-proliferative activities in vitro. Molecules, 26.","DOI":"10.3390\/molecules26082366"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1333","DOI":"10.1111\/jam.15632","article-title":"Evaluating hop extract concentrations found in commercial beer to inhibit Streptococcus mutans biofilm formation","volume":"133","author":"Gregory","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Stompor, M., and \u017barowska, B. (2016). Antimicrobial activity of xanthohumol and its selected structural analogues. Molecules, 21.","DOI":"10.3390\/molecules21050608"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1139\/m75-029","article-title":"Structural features determining the antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives","volume":"21","author":"Schmalreck","year":"1975","journal-title":"Can. J. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2223","DOI":"10.1902\/jop.2005.76.12.2223","article-title":"Apple- and hop-polyphenols protect periodontal ligament cells stimulated with enamel matrix derivative from Porphyromonas gingivalis","volume":"76","author":"Inaba","year":"2005","journal-title":"J. Periodontol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1177\/154405910708600908","article-title":"Hop bract polyphenols reduced three-day dental plaque regrowth","volume":"86","author":"Shinada","year":"2007","journal-title":"J. Dent. Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"110145","DOI":"10.1016\/j.msec.2019.110145","article-title":"Aqueous electrospinning of recombinant spider silk proteins","volume":"106","author":"DeSimone","year":"2020","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1021\/bm050777q","article-title":"Direct in vitro electrospinning with polymer melts","volume":"13","author":"Dalton","year":"2006","journal-title":"Biomacromolecules"},{"key":"ref_70","first-page":"416","article-title":"In vitro biological evaluation of electrospun polycaprolactone\/gelatine nanofibrous scaffold for tissue engineering","volume":"16","author":"Lim","year":"2015","journal-title":"J. Nanomater."},{"key":"ref_71","first-page":"15","article-title":"Effects of Humulus lupulus extract or its components on viability, lipid peroxidation, and expression of vascular endothelial growth factor in melanoma cells and fibroblasts","volume":"1","author":"Philips","year":"2017","journal-title":"J. Clin. Res."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/16\/9\/1258\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:37:38Z","timestamp":1760107058000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/16\/9\/1258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,30]]},"references-count":71,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,5]]}},"alternative-id":["polym16091258"],"URL":"https:\/\/doi.org\/10.3390\/polym16091258","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,30]]}}}