{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T09:09:26Z","timestamp":1785834566256,"version":"3.56.0"},"reference-count":71,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T00:00:00Z","timestamp":1721347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Y\u0131ld\u0131z Technical University Scientific Research Projects Coordination Unit","award":["FBA-2021-4533"],"award-info":[{"award-number":["FBA-2021-4533"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Several therapeutic approaches have been developed to promote bone regeneration, including guided bone regeneration (GBR), where barrier membranes play a crucial role in segregating soft tissue and facilitating bone growth. This study emphasizes the importance of considering specific tissue requirements in the design of materials for tissue regeneration, with a focus on the development of a double-layered membrane to mimic both soft and hard tissues within the context of GBR. The hard tissue-facing layer comprises collagen and zinc-doped bioactive glass to support bone tissue regeneration, while the soft tissue-facing layer combines collagen and chitosan. The electrospinning technique was employed to achieve the production of nanofibers resembling extracellular matrix fibers. The production of nano-sized (~116 nm) bioactive glasses was achieved by microemulsion assisted sol-gel method. The bioactive glass-containing layers developed hydroxyapatite on their surfaces starting from the first week of simulated body fluid (SBF) immersion, demonstrating that the membranes possessed favorable bioactivity properties. Moreover, all membranes exhibited distinct degradation behaviors in various mediums. However, weight loss exceeding 50% was observed in all tested samples after four weeks in both SBF and phosphate-buffered saline (PBS). The double-layered membranes were also subjected to mechanical testing, revealing a tensile strength of approximately 4 MPa. The double-layered membranes containing zinc-doped bioactive glass demonstrated cell viability of over 70% across all tested concentrations (0.2, 0.1, and 0.02 g\/mL), confirming the excellent biocompatibility of the membranes. The fabricated polymer bioactive glass composite double-layered membranes are strong candidates with the potential to be utilized in tissue engineering applications.<\/jats:p>","DOI":"10.3390\/polym16142066","type":"journal-article","created":{"date-parts":[[2024,7,19]],"date-time":"2024-07-19T13:12:57Z","timestamp":1721394777000},"page":"2066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Fabrication of Electrospun Double Layered Biomimetic Collagen\u2013Chitosan Polymeric Membranes with Zinc-Doped Mesoporous Bioactive Glass Additives"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0145-5280","authenticated-orcid":false,"given":"Dilan","family":"Altan","sequence":"first","affiliation":[{"name":"Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, 34220 Istanbul, T\u00fcrkiye"},{"name":"Health Biotechnology Joint Research and Application Center of Excellence, 34903 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4864-0598","authenticated-orcid":false,"given":"Ali Can","family":"\u00d6zarslan","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, 34220 Istanbul, T\u00fcrkiye"},{"name":"Health Biotechnology Joint Research and Application Center of Excellence, 34903 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6288-2091","authenticated-orcid":false,"given":"Cem","family":"\u00d6zel","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, 34220 Istanbul, T\u00fcrkiye"},{"name":"Health Biotechnology Joint Research and Application Center of Excellence, 34903 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7564-9213","authenticated-orcid":false,"given":"Kadriye","family":"Tuzlako\u011flu","sequence":"additional","affiliation":[{"name":"Department of Polymer Engineering, Yalova University, 77200 Yalova, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2119-1216","authenticated-orcid":false,"given":"Yesim Muge","family":"Sahin","sequence":"additional","affiliation":[{"name":"Polymer Technologies and Composite Application and Research Center, Istanbul Arel University, 34537 Istanbul, T\u00fcrkiye"},{"name":"Faculty of Engineering, Department of Biomedical Engineering, Istanbul Arel University, 34537 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9495-9321","authenticated-orcid":false,"given":"Sevil","family":"Y\u00fccel","sequence":"additional","affiliation":[{"name":"Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, 34220 Istanbul, T\u00fcrkiye"},{"name":"Health Biotechnology Joint Research and Application Center of Excellence, 34903 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ren, Y., Fan, L., Alkildani, S., Liu, L., Emmert, S., Najman, S., Rimashevskiy, D., Schnettler, R., Jung, O., and Xiong, X. (2022). Barrier Membranes for Guided Bone Regeneration (GBR): A Focus on Recent Advances in Collagen Membranes. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232314987"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1002\/biot.201100294","article-title":"Polymeric membranes for guided bone regeneration","volume":"6","author":"Gentile","year":"2011","journal-title":"Biotechnol. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/j.dental.2012.04.022","article-title":"Recent advances in the development of GTR\/GBR membranes for periodontal regeneration\u2014A materials perspective","volume":"28","author":"Bottino","year":"2012","journal-title":"Dent. Mater."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1159\/000381699","article-title":"Membranes for Periodontal Regeneration\u2014A Materials Perspective","volume":"17","author":"Bottino","year":"2015","journal-title":"Front. Oral Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1021\/acsmaterialsau.3c00013","article-title":"Resorbable Membranes for Guided Bone Regeneration: Critical Features, Potentials, and Limitations","volume":"3","author":"Abtahi","year":"2023","journal-title":"ACS Mater. Au"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1021\/acs.biomac.9b00159","article-title":"Biocompatibility of Resorbable Polymers: A Historical Perspective and Framework for the Future","volume":"20","author":"Pappalardo","year":"2019","journal-title":"Biomacromolecules"},{"key":"ref_7","first-page":"1","article-title":"Barrier membranes for dental applications: A review and sweet advancement in membrane developments","volume":"2","author":"Rodriguez","year":"2018","journal-title":"Mouth Teeth"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10266-016-0267-0","article-title":"Collagen based barrier membranes for periodontal guided bone regeneration applications","volume":"105","author":"Sheikh","year":"2017","journal-title":"Odontology"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hu, X., He, Y., Tong, Y., Sun, N., Ma, G., Liu, H., and Kou, N. (2024). Fabrication and characterization of a multi-functional GBR membrane of gelatin-chitosan for osteogenesis and angiogenesis. Int. J. Biol. Macromol., 266.","DOI":"10.1016\/j.ijbiomac.2024.130978"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1002\/jbm.b.32662","article-title":"Chitosan as a barrier membrane material in periodontal tissue regeneration","volume":"100B","author":"Xu","year":"2012","journal-title":"J. Biomed. Mater. Res. Part B Appl. Biomater."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5788","DOI":"10.1021\/acsbiomaterials.1c01060","article-title":"Silk Fibroin\/Collagen Blended Membrane Fabricated via a Green Papermaking Method for Potential Guided Bone Regeneration Application: In Vitro and In Vivo Evaluation","volume":"7","author":"Luo","year":"2021","journal-title":"ACS Biomater. Sci. Eng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"117032","DOI":"10.1016\/j.carbpol.2020.117032","article-title":"Biomimetic Janus chitin nanofiber membrane for potential guided bone regeneration application","volume":"251","author":"Prajatelistia","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1002\/adhm.201400302","article-title":"Combining collagen and bioactive glasses for bone tissue engineering: A review","volume":"4","author":"Sarker","year":"2015","journal-title":"Adv. Health Mater."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s10971-023-06264-x","article-title":"Fabrication of polycaprolactone-chitosan\/curcumin polymer composite fibers and evaluation of their in vitro release kinetic behavior and antibacterial-antifungal activity","volume":"109","author":"Ciftci","year":"2024","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31822","DOI":"10.1038\/srep31822","article-title":"Asymmetric Collagen\/chitosan Membrane Containing Minocycline-loaded Chitosan Nanoparticles for Guided Bone Regeneration","volume":"6","author":"Ma","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Forrester, J.V., Dick, A.D., McMenamin, P.G., Roberts, F., and Pearlman, E. (2016). Chapter 4\u2014Biochemistry and cell biology. The Eye, W.B. Saunders. [4th ed.].","DOI":"10.1016\/B978-0-7020-5554-6.00004-6"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"13940","DOI":"10.1016\/j.ceramint.2022.12.276","article-title":"Comprehensive assessment of SrO and CuO co-incorporated 50S6P amorphous silicate bioactive glasses in vitro: Revealing bioactivity properties of bone graft biomaterial for bone tissue engineering applications","volume":"49","year":"2023","journal-title":"Ceram. Int."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zorzi, A.R., and de Miranda, J.B. (2016). Doped Bioactive Glass Materials in Bone Regeneration. Advanced Techniques in Bone Regeneration, IntechOpen.","DOI":"10.5772\/61425"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"33782","DOI":"10.1039\/D0RA04287K","article-title":"Recent advances and future perspectives of sol\u2013gel derived porous bioactive glasses: A review","volume":"10","author":"Deshmukh","year":"2020","journal-title":"RSC Adv."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.actbio.2020.02.022","article-title":"Antibacterial biohybrid nanofibers for wound dresing","volume":"107","author":"Homaeigohar","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_21","first-page":"809","article-title":"Multifunctional role of zinc in human health: An update","volume":"22","author":"Kiouri","year":"2023","journal-title":"EXCLI J."},{"key":"ref_22","first-page":"51","article-title":"Zinc-containing bioactive glasses for bone regeneration, dental and orthopedic applications","volume":"1","author":"Balasubramanian","year":"2015","journal-title":"Biomed. Glas."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/1478-811X-11-74","article-title":"Zinc inhibits osteoclast differentiation by suppression of Ca2+-Calcineurin-NFATc1 signaling pathway","volume":"11","author":"Park","year":"2013","journal-title":"Cell Commun. Signal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5298","DOI":"10.1021\/acs.chemrev.8b00593","article-title":"Electrospinning and electrospun nanofibers: Methods, materials, and applications","volume":"119","author":"Xue","year":"2019","journal-title":"Chem. Rev."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Hanumantharao, S.N., and Rao, S. (2019). Multi-Functional Electrospun Nanofibers from Polymer Blends for Scaffold Tissue Engineering. Fibers, 7.","DOI":"10.3390\/fib7070066"},{"key":"ref_26","first-page":"1146","article-title":"Cross-linking methods of type I collagen-based scaffolds for cartilage tissue engineering","volume":"14","author":"Jiang","year":"2022","journal-title":"J. Transl. Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1007\/s13770-017-0075-9","article-title":"Electrospun Collagen Nanofibers and Their Applications in Skin Tissue Engineering","volume":"14","author":"Law","year":"2017","journal-title":"Tissue Eng. Regen. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"105203","DOI":"10.1016\/j.reactfunctpolym.2022.105203","article-title":"Vapor-phase glutaraldehyde crosslinked waste protein-based nanofiber nonwovens as an environmentally friendly wound dressing","volume":"172","author":"Chen","year":"2022","journal-title":"React. Funct. Polym."},{"key":"ref_29","first-page":"312","article-title":"Multifunctional zinc ion doped sol\u2013gel derived mesoporous bioactive glass nanoparticles for biomedical applications","volume":"4","author":"Zheng","year":"2019","journal-title":"Bioact. Mater."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/978-1-4939-7113-8_19","article-title":"Type I Collagen Purification from Rat Tail Tendons","volume":"1627","year":"2017","journal-title":"Methods Mol. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3490","DOI":"10.1016\/j.matlet.2006.11.104","article-title":"Electrospinning of collagen\u2013chitosan complex","volume":"61","author":"Chen","year":"2007","journal-title":"Mater. Lett."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.actbio.2009.07.024","article-title":"Electrospun collagen\u2013chitosan nanofiber: A biomimetic extracellular matrix for endothelial cell and smooth muscle cell","volume":"6","author":"Chen","year":"2010","journal-title":"Acta Biomater."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhao, X., Gao, J., Hu, X., Guo, H., Wang, F., Qiao, Y., and Wang, L. (2018). Collagen\/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound Dressing. Appl. Sci., 8.","DOI":"10.3390\/app8081226"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.msec.2017.03.037","article-title":"Development of a bioactive glass-polymer composite for wound healing applications","volume":"76","author":"Moura","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1007\/s10856-015-5403-9","article-title":"A unified in vitro evaluation for apatite-forming ability of bioactive glasses and their variants","volume":"26","author":"Kim","year":"2015","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2907","DOI":"10.1016\/j.biomaterials.2006.01.017","article-title":"How useful is SBF in predicting in vivo bone bioactivity?","volume":"27","author":"Kokubo","year":"2006","journal-title":"Biomaterials"},{"key":"ref_37","unstructured":"(2009). Biological Evaluation of Medical Devices\u2014Part 5: Tests for In Vitro Cytotoxicity (Standard No. ISO 10993-5:2009)."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10856-018-6070-4","article-title":"Synthesis and characterization of manganese containing mesoporous bioactive glass nanoparticles for biomedical applications","volume":"29","author":"Nawaz","year":"2018","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"5768","DOI":"10.1021\/acsomega.8b03598","article-title":"Deeper Insights into a Bioactive Glass Nanoparticle Synthesis Protocol To Control Its Morphology, Dispersibility, and Composition","volume":"4","author":"Kesse","year":"2019","journal-title":"ACS Omega"},{"key":"ref_40","first-page":"69","article-title":"Bioactive glasses enriched with zinc and strontium: Synthesis, characterization, cytocompatibility with osteoblasts and antibacterial properties","volume":"25","author":"Chraniuk","year":"2024","journal-title":"Acta Bioeng. Biomech."},{"key":"ref_41","first-page":"91","article-title":"In vitro Bioactivity of Polycaprolactone\/Bioglass Composites","volume":"2013","author":"Radev","year":"2013","journal-title":"Int. J. Mater. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Aguiar, H., Gonz\u00e1lez, P., and Serra, J. (2018). Bioactive glass structure and solubility. Bioactive Glasses, Elsevier.","DOI":"10.1016\/B978-0-08-100936-9.00002-2"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Ahn, J.H., Kim, I.-R., Kim, Y., Kim, D.-H., Park, S.-B., Park, B.-S., Bae, M.-K., and Kim, Y.-I. (2020). The Effect of Mesoporous Bioactive Glass Nanoparticles\/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells. Nanomaterials, 10.","DOI":"10.3390\/nano10040620"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s10971-018-4823-7","article-title":"The influence of cobalt incorporation and cobalt precursor selection on the structure and bioactivity of sol\u2013gel-derived bioactive glass","volume":"88","author":"Barrioni","year":"2018","journal-title":"J. Sol-Gel Sci. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1134\/S0036024406070211","article-title":"Density of silanol groups on the surface of silica precipitated from a hydrothermal solution","volume":"80","author":"Zhuravlev","year":"2006","journal-title":"Russ. J. Phys. Chem. A"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"120755","DOI":"10.1016\/j.jnoncrysol.2021.120755","article-title":"Production of biosilica based bioactive glass-alginate composite putty as bone support material, and evaluation of in vitro properties; bioactivity and cytotoxicity behavior","volume":"561","author":"Elalmis","year":"2021","journal-title":"J. Non-Crystalline Solids"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1021\/jp9939726","article-title":"Inhibitory Effect of Magnesium and Zinc on Crystallization Kinetics of Hydroxyapatite (0001) Face","volume":"104","author":"Kanzaki","year":"2000","journal-title":"J. Phys. Chem. B"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1557\/s43578-023-00908-y","article-title":"Development, structural and rheological characterization, and in vitro evaluation of the zinc-doped 45S5 bioactive glass-vaseline ointment for potential wound healing applications","volume":"38","year":"2023","journal-title":"J. Mater. Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"\u00d6zel, C., \u00c7evlik, C.B., \u00d6zarslan, A.C., Emir, C., Elalmis, Y.B., and Y\u00fccel, S. (2023). Evaluation of biocomposite putty with strontium and zinc co-doped 45S5 bioactive glass and sodium hyaluronate. Int. J. Biol. Macromol., 242.","DOI":"10.1016\/j.ijbiomac.2023.124901"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.ijbiomac.2018.04.129","article-title":"Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation","volume":"115","author":"Gunasekaran","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.msec.2017.05.125","article-title":"Fabrication and characterization of two-layered nanofibrous membrane for guided bone and tissue regeneration application","volume":"80","author":"Rad","year":"2017","journal-title":"Mater. Sci. Eng. C"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.carbpol.2007.09.018","article-title":"Intermolecular interactions in electrospun collagen\u2013chitosan complex nanofibers","volume":"72","author":"Chen","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Klairutsamee, W., Supaphol, P., and Jangchud, I. (2015). Electrospinnability of poly(butylene succinate): Effects of solvents and organic salt on the fiber size and morphology. J. Appl. Polym. Sci., 132.","DOI":"10.1002\/app.42716"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Melo, P., Montalbano, G., Boggio, E., Gigliotti, C.L., Dianzani, C., Dianzani, U., Vitale-Brovarone, C., and Fiorilli, S. (2022). Electrospun Collagen Scaffold Bio-Functionalized with Recombinant ICOS-Fc: An Advanced Approach to Promote Bone Remodelling. Polymers, 14.","DOI":"10.3390\/polym14183780"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"107260","DOI":"10.1016\/j.mtcomm.2023.107260","article-title":"Bilayer nonwovens using natural rubber, poly(lactic acid) and bactericidal nanoparticles for wound dressings","volume":"37","author":"Andre","year":"2023","journal-title":"Mater. Today Commun."},{"key":"ref_56","first-page":"111","article-title":"Electrospun composite polycaprolactone scaffolds for optimized tissue regeneration","volume":"226","author":"Nelson","year":"2012","journal-title":"Proc. Inst. Mech. Eng. Part N J. Nanomater. Nanoeng. Nanosyst."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"3874","DOI":"10.1557\/s43578-023-01105-7","article-title":"Preparation of biosilica glass-based porous scaffolds using various elements (Mg\u2013Zn, Mg\u2013Cu, and Sr\u2013Cu) as bioactive glass co-dopants: In vitro bioactivity and biocompatibility behaviors","volume":"38","year":"2023","journal-title":"J. Mater. Res."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hum, J., and Boccaccini, A.R. (2018). Collagen as Coating Material for 45S5 Bioactive Glass-Based Scaffolds for Bone Tissue Engineering. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19061807"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/j.carbpol.2008.12.039","article-title":"Interaction of anionic collagen with chitosan: Effect on thermal and morphological characteristics","volume":"77","author":"Horn","year":"2009","journal-title":"Carbohydr. Polym."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3114","DOI":"10.1039\/D3MA00010A","article-title":"Advances in electrospun chitosan nanofiber biomaterials for biomedical applications","volume":"4","author":"Tamilarasi","year":"2023","journal-title":"Mater. Adv."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1237","DOI":"10.1002\/jbm.a.34422","article-title":"Water-stable electrospun collagen fibers from a non-toxic solvent and crosslinking system","volume":"101A","author":"Jiang","year":"2013","journal-title":"J. Biomed. Mater. Res. Part A"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.ijbiomac.2015.07.053","article-title":"Wet-spinnability and crosslinked fibre properties of two collagen polypeptides with varied molecular weight","volume":"81","author":"Tronci","year":"2015","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Nair, M., Best, S.M., and Cameron, R.E. (2020). Crosslinking collagen constructs: Achieving cellular selectivity through modifications of physical and chemical properties. Appl. Sci., 10.","DOI":"10.3390\/app10196911"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1080\/09205063.2019.1680927","article-title":"Enhanced effects of electrospun collagen-chitosan nanofiber membranes on guided bone regeneration","volume":"31","author":"Guo","year":"2020","journal-title":"J. Biomater. Sci. Polym. Ed."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Sbricoli, L., Guazzo, R., Annunziata, M., Gobbato, L., Bressan, E., and Nastri, L. (2020). Selection of collagen membranes for bone regeneration: A literature review. Materials, 13.","DOI":"10.3390\/ma13030786"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Islam, M.M., AbuSamra, D.B., Chivu, A., Arg\u00fceso, P., Dohlman, C.H., Patra, H.K., Chodosh, J., and Gonz\u00e1lez-Andrades, M. (2021). Optimization of Collagen Chemical Crosslinking to Restore Biocompatibility of Tissue-Engineered Scaffolds. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13060832"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1080\/17452759.2011.605839","article-title":"Evaluation of in vitro degradation of PCL scaffolds fabricated via BioExtrusion\u2014Part 2: Influence of pore size and geometry: The present study is to accurately investigate the influence of design parameters, such as filament distance (FD) and lay-down pattern, on the degradation behaviour and kinetics of PCL scaffolds, obtained via BioExtrusion","volume":"6","author":"Domingos","year":"2011","journal-title":"Virtual Phys. Prototyp."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Wong, C., Yoganarasimha, S., Carrico, C., and Madurantakam, P. (2019). Incorporation of Fibrin Matrix into Electrospun Membranes for Periodontal Wound Healing. Bioengineering, 6.","DOI":"10.3390\/bioengineering6030057"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S0104-14282011005000008","article-title":"Cytocompatibility of chitosan and collagen-chitosan scaffolds for tissue engineering","volume":"21","author":"Fernandes","year":"2011","journal-title":"Polimeros"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.polymdegradstab.2019.05.023","article-title":"Controlled degradable chitosan\/collagen composite scaffolds for application in nerve tissue regeneration","volume":"166","author":"Si","year":"2019","journal-title":"Polym. Degrad. Stab."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"39322","DOI":"10.1038\/srep39322","article-title":"The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. 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