{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T20:08:14Z","timestamp":1779134894122,"version":"3.51.4"},"reference-count":50,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T00:00:00Z","timestamp":1734998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Council for Scientific and Technological Development (CNPq)","award":["PIBIC 2022\/2023 UFSC"],"award-info":[{"award-number":["PIBIC 2022\/2023 UFSC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>This study aimed to evaluate the antimicrobial effectiveness of different disinfection protocols for dentures by combining methods, varying intervention sequences, sodium hypochlorite (NaOCl) concentrations (0.1% and 0.25%), and post-exposure to intraoral temperature. The heat-polymerized poly(methylmethacrylate) (PMMA) was divided into groups (n = 15): control (C, distilled water immersion), B (brushing), I0.1% and I0.25% (isolated NaOCl immersion), B + I0.1% and B + I0.25% (brushing followed by immersion), I + B0.1% and I + B0.25% (immersion followed by brushing), and B + I0.1% + T and B + I0.25% + T (brushing, NaOCl immersion, and overnight exposure to 35 \u00b0C \u00b1 2 \u00b0C). The post-disinfection exposure to intraoral temperature simulated the denture use during sleeping time. Quantitative evaluation was performed by colony-forming unit (CFU\/mL) counting of C. albicans and qualitative analysis by scanning electron microscopy (SEM) images. Data were processed by one-way ANOVA with Tukey\u2019s post-hoc test to compare different protocols at the same concentration and among groups (\u03b1 \u2264 0.05). Applying 0.25% NaOCl in associated protocols, the intervention sequence was no different (B + I and I + B) and caused the lowest C. albicans counts. The 0.1% NaOCl lost part of its action when the immersion method started the protocols. B + I0.25%, I0.25% + B, and B + I0.1% had similar antimicrobial efficacy, but the intraoral temperature (B + I + T) reduced the efficacy of these protocols, regardless of NaOCl concentration. Residual biofilm recolonization was also detected in SEM images. In conclusion, all the combinations between mechanical and chemical methods using 0.25% NaOCl were the most effective against C. albicans. The antimicrobial efficacy of NaOCl at 0.1% changes depending on the intervention sequence. The intraoral temperature influenced the C. albicans recolonization after the disinfection protocols.<\/jats:p>","DOI":"10.3390\/polym17010008","type":"journal-article","created":{"date-parts":[[2024,12,24]],"date-time":"2024-12-24T10:58:32Z","timestamp":1735037912000},"page":"8","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Disinfection Strategies for Poly(methyl methacrylate): Method Sequence, Solution Concentration, and Intraoral Temperature on Antimicrobial Activity"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9020-3350","authenticated-orcid":false,"given":"Ana Beatriz Sato","family":"Kamio","sequence":"first","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9191-0672","authenticated-orcid":false,"given":"Andressa","family":"da Silva Barboza","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eduarda Broering","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur Ferronato","family":"Soto","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Silva Ribeiro","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thais Mageste","family":"Duque","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariadne Cristiane Cabral","family":"da Cruz","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8034-6284","authenticated-orcid":false,"given":"Ricardo Ruiz","family":"Mazzon","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina (UFSC), Av. Delfino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6118-9116","authenticated-orcid":false,"given":"Maur\u00edcio Malheiros","family":"Badar\u00f3","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Federal University of Santa Catarina (UFSC), Av. Delsino Conti, s\/n\u2014Trindade, Florian\u00f3polis 88040-900, SC, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,24]]},"reference":[{"key":"ref_1","unstructured":"United Nations (2019). World Population Prospects 2019: Highlights, United Nations."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.4103\/ijdr.ijdr_1141_20","article-title":"Relationshipbetween perceived chewing ability, oral health related quality of life and depressive symptoms among completely edentulous individuals","volume":"32","author":"Satishkumar","year":"2021","journal-title":"Indian J. Dent. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zafar, M.S. (2020). Prosthodontic Applications of Polymethyl Methacrylate (PMMA): An Update. 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