{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:03:57Z","timestamp":1790456637013,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control","award":["HPKL2023012"],"award-info":[{"award-number":["HPKL2023012"]}]},{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control","award":["2023275"],"award-info":[{"award-number":["2023275"]}]},{"name":"innovation and entrepreneurship fund for college students of Hunan Normal University","award":["HPKL2023012"],"award-info":[{"award-number":["HPKL2023012"]}]},{"name":"innovation and entrepreneurship fund for college students of Hunan Normal University","award":["2023275"],"award-info":[{"award-number":["2023275"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>It is a great challenge to obtain an ideal hydrogel for the clinical treatment of intrauterine adhesion (IUA) disease. Here, a novel injectable chitosan\u2013lignin\/poloxamer hydrogel loaded with platelet-rich plasma (CL-PF127@PRP) was prepared by self-assembly at room temperature. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rheological analysis, and injectable writing were used to characterize the structure of the hydrogel. The results confirmed that the amino group of chitosan and the sulfonic group of sodium lignosulfonate were ionic-crosslinked by electrostatic attraction, which stabilized the three-dimensional structure of the PF127 hydrogel loaded with PRP, and PRP made the porous structure gradually become tight. Moreover, the CL-PF127@PRP hydrogel displayed good injectability and a solid state. The soaking experiment showed that the CL-PF127@PRP hydrogel had suitable degradation at pH = 7 and a good PRP release rate (PRP release 70% at 96 h). Cell experiments in vitro demonstrated that the CL-PF127@PRP hydrogel possessed good biocompatibility, an anti-inflammatory function, and pro-angiogenic activity. Furthermore, an animal experiment of skin wound and IUA confirmed that the skin wound closure rate of the CL-PF127@PRP hydrogel was over 50% on the seventh day. PRP improved the thickness of the endometrium and uterus receptivity, suggesting that the CL-PF127@PRP hydrogel offers great promise for the clinical treatment of IUA.<\/jats:p>","DOI":"10.3390\/polym17040474","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T11:54:06Z","timestamp":1739274846000},"page":"474","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Study on an Injectable Chitosan\u2013Lignin\/Poloxamer Hydrogel Loaded with Platelet-Rich Plasma for Intrauterine Adhesion Treatment"],"prefix":"10.3390","volume":"17","author":[{"given":"Zhipeng","family":"Yu","sequence":"first","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Min","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Ouyang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuting","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Mao","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuanglin","family":"Xiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3751-430X","authenticated-orcid":false,"given":"Xiang","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory Developmental Biology of Freshwater Fish, School Life Science, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7377-3926","authenticated-orcid":false,"given":"Liuyun","family":"Jiang","sequence":"additional","affiliation":[{"name":"National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3543","DOI":"10.1039\/D3TB03036A","article-title":"Janus adhesive microneedle patch loaded with exosomes for intrauterine adhesion treatment","volume":"12","author":"Wang","year":"2024","journal-title":"J. Mater. Chem. B"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chen, S.S., Lai, J.H., Chen, J.Z., Zheng, L.W., and Wang, M. (2024). 3D printed gelatin\/PTMC core\/shell scaffolds with NIR laser-tuned drug\/biomolecule release for cancer therapy and uterine regeneration. Int. J. Biol. Macromol., 283.","DOI":"10.1016\/j.ijbiomac.2024.137193"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1039\/C9NR09283H","article-title":"Self-healing conductive hydrogels: Preparation, properties and applications","volume":"12","author":"Deng","year":"2020","journal-title":"Nanoscale"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6536","DOI":"10.1039\/D1TB01005K","article-title":"Hydrogel, a novel therapeutic and delivery strategy, in the treatment of intrauterine adhesions","volume":"9","author":"Lv","year":"2021","journal-title":"J. Mater. Chem. B"},{"key":"ref_5","first-page":"82","article-title":"Engineering self-healing adhesive hydrogels with antioxidant properties for intrauterine adhesion prevention","volume":"27","author":"Feng","year":"2023","journal-title":"Bioact. Mater."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cui, N.A., Dai, C.Y., Mao, X.R., Lv, X., Gu, Y., Lee, E.S., Jiang, H.B., and Sun, Y. (2022). Poloxamer-Based Scaffolds for Tissue Engineering Applications: A Review. Gels, 8.","DOI":"10.3390\/gels8060360"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Giuliano, E., Paolino, D., Fresta, M., and Cosco, D. (2018). Mucosal Applications of Poloxamer 407-Based Hydrogels: An Overview. Pharmaceutics, 10.","DOI":"10.3390\/pharmaceutics10030159"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"126342","DOI":"10.1016\/j.cej.2020.126342","article-title":"Study on long-term pest control and stability of double-layer pesticide carrier in indoor and outdoor environment","volume":"403","author":"Huang","year":"2021","journal-title":"Chem. Eng. J."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8213","DOI":"10.1002\/slct.202101829","article-title":"Preparation and Properties of Hydrogels Based on Lignosulfonate and Its Efficiency of Drug Delivery","volume":"6","author":"Li","year":"2021","journal-title":"Chemistryselect"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2515","DOI":"10.1021\/acssuschemeng.0c08022","article-title":"Wood-Derived Hydrogels as a Platform for Drug-Release Systems","volume":"9","author":"Culebras","year":"2021","journal-title":"ACS Sustain. Chem. Eng."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Craciun, A.M., Morariu, S., and Marin, L. (2022). Self-Healing Chitosan Hydrogels: Preparation and Rheological Characterization. Polymers, 14.","DOI":"10.3390\/polym14132570"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10050","DOI":"10.1039\/D0TB01869D","article-title":"Chemical and physical chitosan hydrogels as prospective carriers for drug delivery: A review","volume":"8","author":"Tian","year":"2020","journal-title":"J. Mater. Chem. B"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104475","DOI":"10.1016\/j.surfin.2024.104475","article-title":"Thixotropic chitosan hydrogels for biomedical applications: Unravelling the effect of chitosan concentration on the mechanical behaviour","volume":"50","author":"Enoch","year":"2024","journal-title":"Surf. Interfaces"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"17162","DOI":"10.1039\/C8NJ03482F","article-title":"The chitosan hydrogels: From structure to function","volume":"42","author":"Fu","year":"2018","journal-title":"New J. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2202","DOI":"10.1038\/s41467-019-10243-8","article-title":"Anisotropic and self-healing hydrogels withmulti-responsiveactuating capability","volume":"10","author":"Qin","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5089","DOI":"10.1007\/s10570-020-03145-2","article-title":"Preparation and characterization of bamboo fiber\/chitosan\/nano-hydroxyapatite composite membrane by ionic crosslinking","volume":"27","author":"Tang","year":"2020","journal-title":"Cellulose"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Tang, S., Jiang, L.Y., Jiang, Z.H., Ma, Y.J., Zhang, Y., and Su, S.P. (2023). Preparation and Characterization of a Novel Tragacanth Gum\/Chitosan\/Sr-Nano-Hydroxyapatite Composite Membrane. Polymers, 15.","DOI":"10.3390\/polym15132942"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Rohman, G., Langueh, C., Ramtani, S., Lataillade, J.J., Lutomski, D., Senni, K., and Changotade, S. (2019). The Use of Platelet-Rich Plasma to Promote Cell Recruitment into Low-Molecular-Weight Fucoidan-Functionalized Poly(Ester-Urea-Urethane) Scaffolds for Soft-Tissue Engineering. Polymers, 11.","DOI":"10.3390\/polym11061016"},{"key":"ref_19","first-page":"609","article-title":"The Therapeutic Effect of Intravesical Instillation of Platelet Rich Plasma on Recurrent Bacterial Cystitis in Women: A Randomized Clinical Trial","volume":"16","author":"Mirzaei","year":"2019","journal-title":"Urol. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1097\/PRS.0000000000011164","article-title":"A Pilot, Randomized, Single-Blind, Controlled Study Evaluating the Use of\u2002Platelet Rich Plasma\u2002(PRP) for Hand Skin Rejuvenation","volume":"154","author":"Pincelli","year":"2024","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Seker, S., El\u00e7in, A.E., and El\u00e7in, Y.M. (2025). Current trends in the design and fabrication of PRP-based scaffolds for tissue engineering and regenerative medicine. Biomed. Mater., 20.","DOI":"10.1088\/1748-605X\/ada83f"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"55659","DOI":"10.1021\/acsami.0c17142","article-title":"Improving Chronic Diabetic Wound Healing through an Injectable and Self-Healing Hydrogel with Platelet-Rich Plasma Release","volume":"12","author":"Qian","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"202301370","DOI":"10.1002\/adhm.202301370","article-title":"Platelet Rich Plasma Loaded Multifunctional Hydrogel Accelerates Diabetic Wound Healing via Regulating the Continuously Abnormal Microenvironments","volume":"12","author":"Xu","year":"2023","journal-title":"Adv. Healthc. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"931100","DOI":"10.1093\/humrep\/dead093.1100","article-title":"The effect of subendometrial injection of autologous\u2002platelet rich plasma\u2002(PRP) guided by hysteroscopy in recurrent\u2002implantation failure\u2002patients-A pilot study","volume":"38","author":"Gupta","year":"2023","journal-title":"Hum. Reprod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1002\/ijgo.13353","article-title":"Intrauterine infusion of platelet-rich plasma is a treatment method for patients with intrauterine adhesions after hysteroscopy","volume":"151","author":"Peng","year":"2020","journal-title":"Int. J. Gynecol. Obstet."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/jog.14445","article-title":"Autologous platelet-rich plasma infusion does not improve pregnancy outcomes in frozen embryo transfer cycles in women with history of repeated implantation failure without thin endometrium","volume":"147","author":"Tehraninejad","year":"2021","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_27","first-page":"443","article-title":"Effects of autologous platelet-rich plasma on endometrial expansion in patients undergoing frozen-thawed embryo transfer: A double-blind RCT","volume":"17","author":"Nazari","year":"2019","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1195","DOI":"10.3349\/ymj.2017.58.6.1195","article-title":"Effects of Autologous Platelet-Rich Plasma on Regeneration of Damaged Endometrium in Female Rats","volume":"58","author":"Jang","year":"2017","journal-title":"Yonsei Med. J."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kim, J.H., Park, M., Paek, J.Y., Lee, W.S., Song, H., and Lyu, S.W. (2020). Intrauterine Infusion of Human Platelet-Rich Plasma Improves Endometrial Regeneration and Pregnancy Outcomes in a Murine Model of Asherman\u2019s Syndrome. Front. Physiol., 11.","DOI":"10.3389\/fphys.2020.00105"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Apakupakul, J., Sattasathuchana, P., Chanloinapha, P., and Thengchaisri, N. (2020). Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02350-2"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tan, J., Liang, Y., Sun, L., Yang, Z., Xu, J., Dong, D., and Liu, H. (2023). Degradation Characteristics of Cellulose Acetate in Different Aqueous Conditions. Polymers, 15.","DOI":"10.3390\/polym15234505"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Xu, Z.P., Tang, E.N., and Zhao, H.J. (2019). An Environmentally Sensitive Silk Fibroin\/Chitosan Hydrogel and Its Drug Release Behaviors. Polymers, 11.","DOI":"10.3390\/polym11121980"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.2174\/1871520623666230517141144","article-title":"VEGFR2 Mimicking Peptide Inhibits the Proliferation of Human Umbilical Vein Endothelial Cells (Huvecs) by Blocking VEGF","volume":"23","author":"Ghasemali","year":"2023","journal-title":"Anti-Cancer Agent. Me"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Slyusarenko, M., Nikiforova, N., Sidina, E., Nazarova, I., Egorov, V., Garmay, Y., Merdalimova, A., Yevlampieva, N., Gorin, D., and Malek, A. (2021). Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation. Polymers., 13.","DOI":"10.3390\/polym13030458"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"253","DOI":"10.2144\/000114617","article-title":"Western blot analysis of cells encapsulated in self-assembling peptide hydrogels","volume":"63","author":"Burgess","year":"2017","journal-title":"Biotechniques"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"6840","DOI":"10.1021\/acs.biomac.4c01084","article-title":"Polysaccharide Based Self-Driven Tubular Micro\/Nanomotors as a Comprehensive Platform for Quercetin Loading and Anti-inflammatory Function","volume":"25","author":"Yan","year":"2024","journal-title":"Biomacromolecules."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Lee, J.B., Balikov, D.A., Yang, J.W., Kim, K.S., Park, H.K., Kim, J.K., Kwon, I.K., Bellan, L.M., and Sung, H.-J. (2016). Cationic Nanocylinders Promote Angiogenic Activities of Endothelial Cells. Polymers, 8.","DOI":"10.3390\/polym8010015"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Pahlevanneshan, Z., Deypour, M., Kefayat, A., Rafienia, M., Sajkiewicz, P., and Neisiany, R.E. (2021). Polyurethane-Nanolignin Composite Foam Coated with Propolis as a Platform for Wound Dressing: Synthesis and Characterization. Polymers, 13.","DOI":"10.3390\/polym13183191"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Qi, J., Li, X., Cao, Y., Long, Y., Lai, J., Yao, Y., Meng, Y., Wang, Y., Chen, X.-D., and Vankelecom, H. (2024). Locationally activated PRP via an injectable dual-network hydrogel for endometrial regeneration. Biomaterials, 309.","DOI":"10.1016\/j.biomaterials.2024.122615"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"106195","DOI":"10.1016\/j.clay.2021.106195","article-title":"Novel Ca-SLS-LDH nanocomposites obtained via lignosulfonate modification for corrosion protection of steel bars in simulated concrete pore solution","volume":"211","author":"Chen","year":"2021","journal-title":"Appl. Clay Sci."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/17\/4\/474\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:31:34Z","timestamp":1760027494000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/17\/4\/474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":40,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["polym17040474"],"URL":"https:\/\/doi.org\/10.3390\/polym17040474","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]}}}