{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:06:40Z","timestamp":1792001200979,"version":"4.4.0"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,9]],"date-time":"2025-03-09T00:00:00Z","timestamp":1741478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Science Centre, Poland","award":["DEC-2013\/09\/B\/NZ7\/03978"],"award-info":[{"award-number":["DEC-2013\/09\/B\/NZ7\/03978"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Selenium\u2013polysaccharides possess antioxidant properties, making them promising materials for functional foods, pharmaceuticals, and clinical applications. This study examines the incorporation of selenium into polysaccharides via mycelial biosynthesis and its effects on structure and antioxidant activity. Polysaccharides obtained from Lentinula edodes-submerged cultures grown in Se-supplemented and non-supplemented media were analysed for Se content (RP-HPLC\/FLD), structure (FT-IR, HPLC, and HPGPC-ELSD), and antioxidant activity (DPPH scavenging, reducing power, and Fe2+ chelation). Two low-molecular-weight Se\u2013heteropolysaccharides (Se-FE-1.1 and Se-FE-1.2) containing ~80 and 125 \u00b5g\/g Se were isolated, primarily composed of glucose, mannose, and galactose with \u03b2-glycosidic linkages. Se incorporation into polysaccharides selectively enhanced their antioxidant activity in the DPPH radical scavenging assay, with minimal effects observed in iron chelation and reducing power assays. Crude Se\u2013polysaccharides displayed the highest antioxidant activity, suggesting an additional contribution from protein components. Our findings demonstrate that Se is effectively incorporated into polysaccharides, altering monosaccharide composition while preserving glycosidic linkages. The selective enhancement of radical scavenging suggests that selenium plays a specific role in antioxidant activity, primarily influencing radical scavenging mechanisms rather than interactions with metal ions. Further research is needed to clarify the mechanisms of selenium incorporation, the nature of its bonding within the polysaccharide molecule, and its impact on biological activity.<\/jats:p>","DOI":"10.3390\/polym17060719","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T09:36:24Z","timestamp":1741599384000},"page":"719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Selenium-Enriched Polysaccharides from Lentinula edodes Mycelium: Biosynthesis, Chemical Characterisation, and Assessment of Antioxidant Properties"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7472-0762","authenticated-orcid":false,"given":"Eliza","family":"Malinowska","sequence":"first","affiliation":[{"name":"Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, 1 Banacha Str., 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grzegorz","family":"\u0141apienis","sequence":"additional","affiliation":[{"name":"Department of Functional Polymers and Polymeric Materials, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, 112 Sienkiewicza Str., 90-363 \u0141\u00f3d\u017a, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6010-5478","authenticated-orcid":false,"given":"Agnieszka","family":"Szczepa\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, 1 Banacha Str., 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3575-8631","authenticated-orcid":false,"given":"Jadwiga","family":"Tur\u0142o","sequence":"additional","affiliation":[{"name":"Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, 1 Banacha Str., 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Di Carlo, E., and Sorrentino, C. 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