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In this study, we developed a novel Janus membrane by modifying a polypropylene (PP) fibrous membrane with dopamine and zinc oxide nanoparticles (ZnO-NPs). Fabricated via a simple floating immersion method, this asymmetric bilayer structure consists of a hydrophobic PP layer and a hydrophilic PP\/dopamine@30 nm ZnO layer, providing both antibacterial properties and enhanced fluid filtration. The mechanical properties of the PP\/ZnO membrane were significantly enhanced, with an increase in the Young\u2019s modulus and ultimate tensile strength, indicating improved strength. Antibacterial activity against Streptococcus mutans (S. mutans) demonstrated a significant reduction in biofilm formation on the PP\/dopamine@30 nm ZnO surface compared to unmodified PP. Water flux tests confirmed a stable, high filtration rate, with increased permeability under rising pressure. In vivo experiments with miniature pigs confirmed reduced bacterial presence on the sterile side of the membrane. These findings highlight the potential of the membrane for oral exudate filtration, extending filtration time and minimizing infection risks under strict sterility conditions. Further improvements in barrier properties are necessary to optimize its clinical performance.<\/jats:p>","DOI":"10.3390\/polym17101356","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T09:58:27Z","timestamp":1747303107000},"page":"1356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Bioinspired Janus Membrane with Dopamine-ZnO Coating for Antibacterial Filtration in Oral Applications"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6427-2118","authenticated-orcid":false,"given":"Yumeng","family":"Guo","sequence":"first","affiliation":[{"name":"School of Stomatology, Capital Medical University, Beijing 100070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9374-4733","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Stomatology, Capital Medical University, Beijing 100070, China"},{"name":"Department of Stomatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0818-4708","authenticated-orcid":false,"given":"Guoming","family":"Sun","sequence":"additional","affiliation":[{"name":"Zhejiang Sci-Tech University Shengzhou Innovation Research Institute, Shengzhou 312400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7913-0032","authenticated-orcid":false,"given":"Ying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"140853","DOI":"10.1016\/j.cej.2022.140853","article-title":"Janus fibrous membrane with directional liquid transport capacity for wound healing promotion","volume":"455","author":"Pi","year":"2023","journal-title":"Chem. 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