{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:43:28Z","timestamp":1783647808015,"version":"3.55.0"},"reference-count":39,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>This study aimed to evaluate the impact of UVC (Ultraviolet C Radiation), detergent foam, and alcohol (70%) sterilization methods on the surface morphology of acrylonitrile\u2013butadiene\u2013styrene (ABS) specimens using a novel SEM (Scanning Electron Microscope) image processing approach. Twelve 3D-printed specimens were prepared, and five concentric circular regions of interest (ROIs) per specimen were analyzed. Three quantitative descriptors\u2014defect area fraction, anisotropy ratio, and RMS (Root Mean Square) roughness\u2014were extracted to assess surface alterations. To validate the image-based findings, EDX (Energy-Dispersive X-ray Spectroscopy) elemental analysis for carbon (C), nitrogen (N), and oxygen (O) was employed as a complementary and traditional benchmark technique. Statistical comparisons and p-value heat maps revealed strong convergence between SEM and EDX results. UVC sterilization consistently preserved surface morphology and elemental stability, showing the lowest defect fraction (p = 0.2684), balanced anisotropy (p = 0.02481), and minimal oxygen incorporation (O = 7.6). Foam sterilization produced intermediate effects, with significant anisotropy changes (p = 0.007456) and reduced nitrogen (19.6). Alcohol sterilization induced the most severe damage, characterized by high defect density, increased roughness, and elemental imbalance (N = 17.3, O = 13.9), confirming oxidative degradation. The convergence of SEM and EDX outcomes demonstrates that SEM image processing is a reliable novel method validated by traditional elemental analysis. Together, these approaches provide a robust framework for ranking sterilization efficacy, with UVC identified as the most favorable method, detergent foam as an acceptable alternative, and alcohol as the least effective due to its destabilizing effects.<\/jats:p>","DOI":"10.3390\/polym17233156","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T16:31:52Z","timestamp":1764261112000},"page":"3156","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Novel SEM Image Processing Approach for Evaluating Sterilization Effects on Polymeric Medical Devices: Validation Against Traditional EDX Analysis"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4433-776X","authenticated-orcid":false,"given":"Mohamed A.","family":"Aboamer","sequence":"first","affiliation":[{"name":"Department of Medical Equipment Technology, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashed","family":"Almousa","sequence":"additional","affiliation":[{"name":"Department of Medical Equipment Technology, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8825-6681","authenticated-orcid":false,"given":"Ahmad","family":"Alassaf","sequence":"additional","affiliation":[{"name":"Department of Medical Equipment Technology, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrahman","family":"Alduraywish","sequence":"additional","affiliation":[{"name":"Department of Medical Equipment Technology, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim","family":"AlMohimeed","sequence":"additional","affiliation":[{"name":"Department of Medical Equipment Technology, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7888-7105","authenticated-orcid":false,"given":"Talal","family":"Alharbi","sequence":"additional","affiliation":[{"name":"Department of Electrical Engineering, College of Engineering, Qassim University, Buraydah 52531, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidan F.","family":"Ghoneim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, College of Engineering, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2025, August 27). Report on the Burden of Endemic Health Care-Associated Infection Worldwide, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK144030\/."},{"key":"ref_2","unstructured":"Modjarrad, K., and Ebnesajjad, S. (2013). Application of Plastics in Medical Devices and Equipment. Handbook of Polymer Applications in Medicine and Medical Devices, Elsevier."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1007\/s12206-022-0616-6","article-title":"A Comparative Study of Mechanical Behavior of ABS Material Based on UVC Sterilization for Medical Usage","volume":"36","author":"Aboamer","year":"2022","journal-title":"J. Mech. Sci. 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