{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T21:54:41Z","timestamp":1770155681437,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cSirius\u201d Federal Territory, Scientific and Technological Development of the \u201cSirius\u201d Federal Territory","award":["18-03"],"award-info":[{"award-number":["18-03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Despite metals currently being widely used in orthopedic surgery, their mechanical properties significantly differ from the surrounding tissues and organs, causing low biocompatibility. Biodegradable, non-toxic, and non-immunogenic materials seem to be more convenient for clinical implementation. Our research was aimed at the construction of a polylactide screw covered with collagen, nanohydroxyapatite, and polylactide, with a variant including silver nanowires for antibacterial properties, as well as the analysis of their physico-chemical and biological properties. Adherent human osteosarcoma cells (2T line) were shown to grow on the porous surface layers. A cytotoxicity assay using WST1 revealed the non-toxic nature of the coatings and showed an increase in cell adhesion and proliferation. Safety and efficacy were also evaluated in vivo with the coated screws implanted into the metatarsal bones of minipigs. Histological analysis at 29 and 58 days post-screw-implantation revealed that the coated samples accelerated bone tissue regeneration compared to uncoated controls. This was evidenced by a higher bone-to-granulation tissue ratio, reduced inflammatory cell counts, and increased osteoblast\/osteoclast activity at the early stage during the initial days after implantation. The results confirm that the developed bioactive coatings enhance biocompatibility and osteointegration.<\/jats:p>","DOI":"10.3390\/polym18010052","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:22:27Z","timestamp":1766578947000},"page":"52","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioactive Surface Modifications on Bioresorbable Bone Screws: A Step Forward in Orthopedic Surgery"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1414-7786","authenticated-orcid":false,"given":"Ainur G.","family":"Matveyeva","sequence":"first","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6246-7085","authenticated-orcid":false,"given":"Olga P.","family":"Boychenko","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"},{"name":"Department of Chemistry, Lomonosov Moscow State University (MSU), 119991 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7202-0268","authenticated-orcid":false,"given":"Alexander P.","family":"Moskalets","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey S.","family":"Zakakuev","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0512-2547","authenticated-orcid":false,"given":"Nikolay A.","family":"Barinov","sequence":"additional","affiliation":[{"name":"Scientific Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sochi, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra S.","family":"Bogdanova","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9630-0777","authenticated-orcid":false,"given":"Olga V.","family":"Morozova","sequence":"additional","affiliation":[{"name":"Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitry V.","family":"Klinov","sequence":"additional","affiliation":[{"name":"Scientific Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sochi, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5905-2652","authenticated-orcid":false,"given":"Dimitri A.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Scientific Center for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sochi, Russia"},{"name":"Institut de Sciences des Mat\u00e9riaux de Mulhouse (CNRS UMR 7361), Universit\u00e9 de Haute Alsace, 68100 Mulhouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1053\/ar.1999.v15.0150811","article-title":"Absorbable and metal interference screws: Comparison of graft security during healing","volume":"15","author":"Walton","year":"1999","journal-title":"Arthrosc. J. Arthrosc. Relat. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/BF00443385","article-title":"Biodegradable implants in traumatology: A review on the state-of-the-art","volume":"114","author":"Hofmann","year":"1995","journal-title":"Arch. Orthop. Trauma Surg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3109\/17453678008990789","article-title":"Recovery of diaphyseal bone strength after rigid internal plate fixation: An experimental study in the rabbit","volume":"51","author":"Sigurdsson","year":"1980","journal-title":"Acta Orthop. Scand."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"718","DOI":"10.3109\/17453678909149613","article-title":"Stress shielding by rigid fixation studied in osteotomized rabbit tibiae","volume":"60","author":"Nilsson","year":"1989","journal-title":"Acta Orthop. Scand."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.2106\/00004623-198870090-00015","article-title":"Refracture of bones of the forearm after the removal of compression plates","volume":"70","author":"Deluca","year":"1988","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.2106\/00004623-198466080-00012","article-title":"Refracture of bones of the forearm after plate removal","volume":"66","author":"Hidaka","year":"1984","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1016\/S0015-0282(16)42920-1","article-title":"Comparison of Polyglactic and Polyglycolic Acid Sutures in Reproductive Tissue","volume":"28","author":"Riddick","year":"1977","journal-title":"Fertil. Steril."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1016\/S0140-6736(85)91847-1","article-title":"Biodegradable implants in fracture fixation: Early results of treatment of fractures of the ankle","volume":"325","author":"Rokkanen","year":"1985","journal-title":"Lancet"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/0142-9612(87)90034-2","article-title":"Resorbable materials of poly(L-lactide). VI. Plates and screws for internal fracture fixation","volume":"8","author":"Leenslag","year":"1987","journal-title":"Biomaterials"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0142-9612(87)90121-9","article-title":"Resorbable materials of poly(L-lactide). VII. In vivo and in vitro degradation","volume":"8","author":"Leenslag","year":"1987","journal-title":"Biomaterials"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1302\/0301-620X.96B4.32171","article-title":"A prospective, randomised trial comparing the use of absorbable and metallic screws in the fixation of distal tibiofibular syndesmosis injuries: Mid-term follow-up","volume":"96-B","author":"Sun","year":"2014","journal-title":"Bone Jt. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"26","DOI":"10.2106\/00004623-200201000-00005","article-title":"Treatment of syndesmotic disruptions of the ankle with bioabsorbable screw fixation","volume":"84","author":"Hovis","year":"2002","journal-title":"J. Bone Jt. Surg. Am."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.1177\/0363546510361952","article-title":"A long-term, prospective, randomized study comparing biodegradable and metal interference screws in anterior cruciate ligament reconstruction surgery: Radiographic results and clinical outcome","volume":"38","author":"Stener","year":"2010","journal-title":"Am. J. Sports Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1007\/s00167-010-1353-4","article-title":"A prospective randomized study of ACL-reconstructions using bone-patellar tendon-bone grafts fixed with bioabsorbable or metal interference screws","volume":"19","author":"Drogset","year":"2011","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S0749-8063(00)90055-0","article-title":"Biodegradable implants in sports medicine: The biological base","volume":"16","author":"Weiler","year":"2000","journal-title":"Arthrosc. J. Arthrosc. Relat. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1586\/17434440.2.1.87","article-title":"Bone reconstruction: From bioceramics to tissue engineering","volume":"2","year":"2005","journal-title":"Expert Rev. Med. Devices"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1007\/s10856-006-0061-6","article-title":"The effect of tri-calcium phosphate (TCP) addition on the degradation of polylactide-co-glycolide (PLGA)","volume":"19","author":"Ehrenfried","year":"2008","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3900","DOI":"10.1002\/adma.200901093","article-title":"The Influence of \u03b1-Tricalcium Phosphate Nanoparticles and Microparticles on the Degradation of Poly(D,L-lactide-co-glycolide)","volume":"21","author":"Yang","year":"2009","journal-title":"Adv. Mater."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1016\/j.actbio.2011.11.028","article-title":"Mineral coatings modulate \u03b2-TCP stability and enable growth factor binding and release","volume":"8","author":"Lee","year":"2012","journal-title":"Acta Biomater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.arthro.2007.10.004","article-title":"Long-term absorption of \u03b2-tricalcium phosphate poly-L-lactic acid interference screws","volume":"24","author":"Barber","year":"2008","journal-title":"Arthrosc. J. Arthrosc. Relat. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/s11751-009-0063-2","article-title":"Magnetic resonance imaging analysis of the bioabsorbable Milagro\u2122 interference screw for graft fixation in anterior cruciate ligament reconstruction","volume":"4","author":"Frosch","year":"2009","journal-title":"Strateg. Trauma Limb Reconstr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1007\/s00167-008-0528-8","article-title":"Polymer-hydroxyapatite composite versus polymer interference screws in anterior cruciate ligament reconstruction in a large animal model","volume":"16","author":"Hunt","year":"2008","journal-title":"Knee Surg. Sports Traumatol. Arthrosc."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1016\/j.arthro.2011.06.036","article-title":"Resorption and remodeling of hydroxyapatite\u2013poly-L-lactic acid composite anterior cruciate ligament interference screws","volume":"27","author":"Johnston","year":"2011","journal-title":"Arthrosc. J. Arthrosc. Relat. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"110423","DOI":"10.1016\/j.msec.2019.110423","article-title":"Clinical complications of biodegradable screws for ligament injuries","volume":"109","author":"Ramos","year":"2020","journal-title":"Mater. Sci. Eng. C Mater. Biol. Appl."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4518","DOI":"10.1021\/am201212u","article-title":"New Approach to Bone Tissue Engineering: Simultaneous Application of Hydroxyapatite and Bioactive Glass Coated on a Poly(l-lactic acid) Scaffold","volume":"3","author":"Dinarvand","year":"2011","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Tsareva, A.D., Shtol, V.S., Klinov, D.V., and Ivanov, D.A. (2025). Electrospinning for Biomedical Applications: An Overview of Material Fabrication Techniques. Surfaces, 8.","DOI":"10.3390\/surfaces8010007"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.jmst.2013.03.002","article-title":"Biodegradable Materials for Bone Repairs: A Review","volume":"29","author":"Tan","year":"2013","journal-title":"J. Mater. Sci. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1038\/208365a0","article-title":"Intermediate States in the Precipitation of Hydroxyapatite","volume":"208","author":"Eanes","year":"1965","journal-title":"Nature"},{"key":"ref_29","unstructured":"(2006). Sterilization of Health Care Products\u2014Radiation. Standard No. GOST ISO 11137-1."},{"key":"ref_30","unstructured":"The European Parliament and the Council of the European Union (2010). Directive 2010\/63\/EU of the European Parliament and of the Council. Off. J. Eur. Union, 53, L276."},{"key":"ref_31","unstructured":"Committee for the Update of the Guide for the Care and Use of Laboratory Animals, Institute for Laboratory Animal Research, Division on Earth and Life Studies, and National Research Council of The National Academies (2011). Guide for the Care and Use of Laboratory Animals, The National Academies Press. [8th ed.]."},{"key":"ref_32","first-page":"88","article-title":"Effect of fixing liquids on the microscopic structure of organs of small laboratory animals","volume":"3","author":"Gushchin","year":"2014","journal-title":"Int. J. Vet. Med."},{"key":"ref_33","first-page":"103","article-title":"Peculiarities of histological processing of organs and tissues of laboratory animals","volume":"2","author":"Muzhikyan","year":"2014","journal-title":"Int. Bull. Vet. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"12446","DOI":"10.1038\/s41598-020-69455-4","article-title":"Comparison of bone-to-implant contact and bone volume around implants placed with or without site preparation: A histomorphometric study in rabbits","volume":"10","author":"Folkman","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"6507","DOI":"10.2147\/IJN.S119519","article-title":"Biomechanical and histological evaluation of the osseointegration capacity of two types of zirconia implant","volume":"11","author":"Han","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"15436","DOI":"10.1038\/s41598-021-94886-y","article-title":"Osseodensification enables bone healing chambers with improved low-density bone site primary stability: An in vivo study","volume":"11","author":"Sartoretto","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_37","first-page":"168","article-title":"Dwarf pigs as an object of preclinical studies","volume":"3","author":"Rybakova","year":"2016","journal-title":"Int. Bull. Vet. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1002\/jor.24852","article-title":"An overview of de novo bone generation in animal models","volume":"39","author":"Taguchi","year":"2021","journal-title":"J. Orthop. Res."},{"key":"ref_39","first-page":"76","article-title":"Comparative bone anatomy of commonly used laboratory animals: Implications for drug discovery","volume":"61","author":"Bagi","year":"2011","journal-title":"Comp. Med."},{"key":"ref_40","first-page":"15","article-title":"Growth and skeletal development of the pig","volume":"358","author":"Reiland","year":"1978","journal-title":"Acta Radiol. Suppl."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.semcdb.2008.07.004","article-title":"Bone remodeling during fracture repair: The cellular picture","volume":"19","author":"Schindeler","year":"2008","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.bone.2016.02.020","article-title":"Inflammation, fracture and bone repair","volume":"86","author":"Loi","year":"2016","journal-title":"Bone"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6055","DOI":"10.1038\/s41598-021-84888-1","article-title":"Computational modeling reveals a key role for polarized myeloid cells in controlling osteoclast activity during bone injury repair","volume":"11","author":"Lo","year":"2021","journal-title":"Sci. Rep."}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/18\/1\/52\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T09:12:17Z","timestamp":1770109937000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/18\/1\/52"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,24]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["polym18010052"],"URL":"https:\/\/doi.org\/10.3390\/polym18010052","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,24]]}}}