{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T08:51:58Z","timestamp":1782291118989,"version":"3.54.5"},"reference-count":75,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Baiyunshan Hejigong Pharmaceutical Factory, Guangzhou Baiyunshan Pharmaceutical Holdings Co., Ltd.","award":["KH25138"],"award-info":[{"award-number":["KH25138"]}]},{"name":"Key Laboratory Project of Guangdong Province","award":["2022B1212010015"],"award-info":[{"award-number":["2022B1212010015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Natural polysaccharides from Siegesbeckia orientalis L. have been reported to exhibit promising bioactivities. To enhance extraction efficiency, low-temperature plasma-assisted extraction was optimized for S. orientalis L. polysaccharides using single-factor experiments and response surface methodology. Column chromatography purified a homogeneous SIE-III fraction, followed by structural characterization. Optimal parameters were 80 kV discharge voltage, 153 Hz frequency, and 109 s treatment time, under which the polysaccharide yield reached 15.68%, significantly higher than that of the conventional hot water extraction method. Plasma treatment loosened the raw material\u2019s surface, potentially facilitating polysaccharide release. SIE-III had a molecular weight of 20.831 kDa and comprised mainly galactose (51.7%), rhamnose (19.1%), arabinose (11.3%), and galacturonic acid (9.9%). It featured typical rhamnogalacturonan-I (RG-I) domains and a triple-helix conformation. Fourier transform infrared spectroscopy and nuclear magnetic resonance confirmed both \u03b1- and \u03b2- glycosidic linkages, and methylation analysis revealed a highly branched \u21923,4)-Galp-(1\u2192 structure. This study provides an effective extraction method for plant polysaccharides and valuable insights into their potential applications in the food and other industries.<\/jats:p>","DOI":"10.3390\/polym18131568","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T07:57:24Z","timestamp":1782287844000},"page":"1568","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Plasma-Assisted Extraction of Polysaccharides from Siegesbeckia orientalis L.: Optimization, Purification, and Structural Characterization"],"prefix":"10.3390","volume":"18","author":[{"given":"Yong-Hua","family":"Li","sequence":"first","affiliation":[{"name":"Baiyunshan Hejigong Pharmaceutical Factory, Guangzhou Baiyunshan Pharmaceutical Holdings Co., Ltd., Guangzhou 510410, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Jie","family":"Zeng","sequence":"additional","affiliation":[{"name":"Baiyunshan Hejigong Pharmaceutical Factory, Guangzhou Baiyunshan Pharmaceutical Holdings Co., Ltd., Guangzhou 510410, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Yun","family":"Wu","sequence":"additional","affiliation":[{"name":"Baiyunshan Hejigong Pharmaceutical Factory, Guangzhou Baiyunshan Pharmaceutical Holdings Co., Ltd., Guangzhou 510410, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Meng","sequence":"additional","affiliation":[{"name":"Baiyunshan Hejigong Pharmaceutical Factory, Guangzhou Baiyunshan Pharmaceutical Holdings Co., Ltd., Guangzhou 510410, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Na","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Yi","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4136-6044","authenticated-orcid":false,"given":"Yan-Yan","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, School of Food Science and Engineering, Foshan University, Foshan 528225, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7480-5075","authenticated-orcid":false,"given":"Feng-Song","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"ref_1","first-page":"23","article-title":"A Detailed Review on Siegesbeckia orientalis","volume":"8","author":"Sukanya","year":"2022","journal-title":"Microscopy"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Chu, J.M.T., Abulimiti, A., Wong, B.S.H., Zhao, G.D., Xiong, S.H., Zhao, M.M., Wang, Y., Chen, Y., Wang, J., and Zhang, Y. 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