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Additional surface modification may improve their interaction with the wound environment and influence tissue repair mechanisms. TMC\/CS nanofibers were fabricated via electrospinning and subsequently processed into three formulations: unloaded fibers (NCC), norfloxacin-loaded fibers (NCX), and norfloxacin-loaded fibers modified with 2-formylphenylboronic acid (NCXA). The resulting materials were characterized using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and UV\u2013Vis spectroscopy. Their therapeutic performance was evaluated in a standardized deep dermal burn model in Wistar rats, with Vaseline gauze and silver sulfadiazine serving as reference treatments. Wound healing progression was assessed through macroscopic examination, histopathological analysis, immunohistochemical evaluation of TNF-\u03b1, IL-1\u03b2, IL-17, VEGF, VCAM, and CD163 expression, and systemic IL-8 determination. Physicochemical characterization confirmed homogeneous nanofiber formation, efficient incorporation of norfloxacin, and successful surface modification. All electrospun formulations promoted improved healing outcomes compared with the untreated control group. Among them, the norfloxacin-loaded nanofiber formulation demonstrated the most pronounced wound-healing effect, characterized by faster re-epithelialization, attenuation of inflammatory mediators during later healing stages, and superior tissue architecture restoration. Conversely, the 2-formylphenylboronic acid-modified norfloxacin-loaded fiber formulation maintained a more persistent inflammatory state and exhibited a slower transition into the remodeling phase. Trimethyl chitosan-based nanofibers loaded with norfloxacin show strong potential as multifunctional wound dressing platforms capable of controlled drug release. The findings indicate that formulation composition plays a critical role in regulating inflammation and tissue regeneration, underscoring the need for continued refinement of chitosan-derived nanosystems for burn wound therapy.<\/jats:p>","DOI":"10.3390\/polym18131621","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T08:26:15Z","timestamp":1782807975000},"page":"1621","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Functional Electrospun Chitosan-Based Nanofibers Loaded with Norfloxacin for Enhanced Burn Wound Healing Response"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0028-3322","authenticated-orcid":false,"given":"Corneliu-George","family":"Coman","sequence":"first","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania"},{"name":"Department of Surgery, Faculty of Medicine, Pharmacy and Biomedical Sciences, University of Mons, 7000 Mons, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6071-7618","authenticated-orcid":false,"given":"Ioannis","family":"Gardikiotis","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania"},{"name":"Sfanta Maria Children\u2019s Emergency Hospital Iasi, 700309 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0323-0296","authenticated-orcid":false,"given":"Carmen","family":"Solcan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Ion Ionescu de la Brad University of Life Sciences, 700490 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2343-5782","authenticated-orcid":false,"given":"Cosmin-Gabriel","family":"Tartau","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Chabot","sequence":"additional","affiliation":[{"name":"Department of Radiology, Cliniques Universitaires Saint Luc, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5551-6077","authenticated-orcid":false,"given":"Gianina","family":"Dodi","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-3811","authenticated-orcid":false,"given":"Liliana Mititelu","family":"Tartau","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Greaves, I. (2009). Trauma Care Manual, Hodder Arnold. [2nd ed.].","DOI":"10.1201\/b13361"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Foster, K.N., and Caruso, D.M. (2016). Burn Resuscitation, An Issue of Critical Care Clinics, Elsevier. The Clinics: Internal Medicine; 1. Aufl.","DOI":"10.1016\/S0749-0704(16)30068-9"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"\u017bwiere\u0142\u0142o, W., Piorun, K., Sk\u00f3rka-Majewicz, M., Maruszewska, A., Antoniewski, J., and Gutowska, I. (2023). Burns: Classification, Pathophysiology, and Treatment: A Review. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24043749"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ahmad, N. (2022). 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