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In the present study, an electrospun membrane composed of polyvinyl alcohol\/kefiran\/schizophyllan loaded with gentamicin and ascorbic acid was developed. Kefiran was obtained from kefir with a 0.61% extraction yield. Beadless electrospun membranes with a diameter of 400 nm were obtained. Antimicrobial activity of the membrane against Staphylococcus aureus and Pseudomonas aeruginosa was determined. Growth inhibition halos of 16.4 \u00b1 2.2 mm were found for Pseudomonas aeruginosa. Furthermore, the membrane cytocompatibility assay of the membrane showed no cell toxicity in human dermal fibroblasts (HDFn cells). The produced membranes showed potential to be used as a wound dressing material in the future.<\/jats:p>","DOI":"10.3390\/polym18131679","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T13:19:31Z","timestamp":1783430371000},"page":"1679","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Gentamicin-Loaded Electrospun PVA\/Kefiran\/Schizophyllan Membrane for Skin Tissue Engineering Applications"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6716-8604","authenticated-orcid":false,"given":"Karla Katiushka","family":"Sol\u00eds-Ar\u00e9valo","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Av. Pedro de Alba S\/N, San Nicol\u00e1s de los Garza C.P. 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis J.","family":"Gal\u00e1n-Wong","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Av. Pedro de Alba S\/N, San Nicol\u00e1s de los Garza C.P. 66455, Nuevo Le\u00f3n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"Rodriguez-Garcia","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n, Facultad de Ciencias Biol\u00f3gicas, Instituto de Biotecnolog\u00eda, Ciudad Universitaria, Av. 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