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Chitosan solutions were characterized in acidulated aqueous medium by determining changes in pH, electrical conductivity, zeta potential, surface tension, viscosity, transmittance, particle size, and polydispersity index (PDI) with respect to polymer concentration. Subsequently, nanoliposomes were developed using an ethanol injection method assisted by ultra-high-pressure homogenization (UHPH) prior to coating. The characterization of the vesicular systems involved particle size analysis, polydispersity index (PDI), and zeta potential, which were evaluated at zero time and at the fourth week prior to storage at 4 \u00b0C and 40 \u00b0C. The physicochemical characterization of chitosan solutions displayed an inflection point at 1 \u00d7 10\u22123 M, which was taken as the coating concentration. In general, non-coated liposomes ranged between 150 and 200 nm, with low polydispersity (&lt;0.3) and negative zeta potentials around ~\u221243 mV. Chitosan coating significantly increased particle size and PDI, while decreasing zeta potential values, but within the same negative value range, suggesting a slight interfacial coating effect. Finally, the systems subjected to UHPH and coated with high- and medium-molecular-weight chitosan showed interesting stabilization against storage conditions in the thermal stress tests.<\/jats:p>","DOI":"10.3390\/polym18151853","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T08:16:52Z","timestamp":1785313012000},"page":"1853","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Physicochemical and Stability Study of Chitosan-Coated Nanoliposomes: Effects of Polymer Molecular Weight and Ultrahigh Pressure Homogenization-UHPH"],"prefix":"10.3390","volume":"18","author":[{"given":"Mariana","family":"Sierra","sequence":"first","affiliation":[{"name":"Grupo Natura, Departamento de Ciencias Farmac\u00e9uticas y Qu\u00edmicas, Facultad Barberi de Ingenier\u00eda, Dise\u00f1o y Ciencias Aplicadas, Universidad Icesi, Calle 18 No. 122-135, Cali 760031, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-6286","authenticated-orcid":false,"given":"Sandra","family":"Navarro-Gall\u00f3n","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n Cecoltec, Cecoltec Services, Cra. 43A #18 Sur-135, Medell\u00edn 050022, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L.","family":"Giraldo","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Facultad de Ciencias Farmac\u00e9uticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yhors","family":"Ciro","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Qu\u00edmica y Biotecnolog\u00eda (QUIBIO), Facultad de Ciencias B\u00e1sicas, Universidad Santiago de Cali, Cali 760035, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1587-5371","authenticated-orcid":false,"given":"Constain H.","family":"Salamanca","sequence":"additional","affiliation":[{"name":"Departamento de Farmacia, Facultad de Ciencias Farmac\u00e9uticas y Alimentarias, Universidad de Antioquia, Calle 70 No. 52-21, Medell\u00edn 050010, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1039\/D5PM00179J","article-title":"Advancements in Nano-Based Drug Delivery Systems for Therapeutics: A Comprehensive Review","volume":"3","author":"John","year":"2026","journal-title":"RSC Pharm."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Morales, J., Fimbres-Olivarr\u00eda, D., Gonz\u00e1lez-Vega, R.I., Bernal-Mercado, A.T., Aubourg-Mart\u00ednez, S.P., L\u00f3pez-Gast\u00e9lum, K.A., Robles-Garc\u00eda, M.\u00c1., de Jes\u00fas Ornelas-Paz, J., Ruiz-Cruz, S., and Del-Toro-S\u00e1nchez, C.L. (2025). Nanoliposomes as Effective Vehicles of Antioxidant Compounds in Food and Health. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202504.2219.v1"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e112274","DOI":"10.5812\/amh.112274","article-title":"A Brief Review on Advantages of Nano-Based Drug Delivery Systems","volume":"19","author":"Hami","year":"2021","journal-title":"Ann. Mil. Health Sci. Res."},{"key":"ref_4","unstructured":"Rajendran, R., and Thomas, S. (2026). Self-Assembly of Lipid Particles for the Delivery of Nutraceuticals and Nanonutraceuticals BT\u2014Handbook of Nutraceuticals: Science, Technology and Engineering, Springer Nature."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Landi, C., Landucci, E., Mazzantini, C., Castellacci, R., and Bergonzi, M.C. (2025). Vitamin D Nanoliposomes to Improve Solubility, Stability, and Uptake Across Intestinal Barrier. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17101244"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"108222","DOI":"10.1016\/j.jddst.2026.108222","article-title":"Transdermal Penetration and Formulation Applicability of Glabridin Nanoliposomes","volume":"120","author":"Xu","year":"2026","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Gil-Gonzalo, R., Durante-Salmer\u00f3n, D.A., Pouri, S., Doncel-P\u00e9rez, E., Alc\u00e1ntara, A.R., Aranaz, I., and Acosta, N. (2024). Chitosan-Coated Liposome Formulations for Encapsulation of Ciprofloxacin and Etoposide. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16081036"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e34554","DOI":"10.1016\/j.heliyon.2024.e34554","article-title":"Chitosan-Coated Liposomal Systems for Delivery of Antibacterial Peptide LL17-32 to Porphyromonas Gingivalis","volume":"10","author":"Han","year":"2024","journal-title":"Heliyon"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Mazzotta, E., Marazioti, A., Mourtas, S., Muzzalupo, R., and Antimisiaris, S.G. (2024). Liposomes Coated with Novel Synthetic Bifunctional Chitosan Derivatives as Potential Carriers of Anticancer Drugs. Pharmaceutics, 16.","DOI":"10.3390\/pharmaceutics16030319"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Pacheco, F., Barrera, A., Ciro, Y., Polo-Cer\u00f3n, D., Salamanca, C.H., and O\u00f1ate-Garz\u00f3n, J. (2024). 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Foods, 14.","DOI":"10.3390\/foods14060968"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"113946","DOI":"10.1016\/j.lwt.2022.113946","article-title":"Improving the Stability and Bioactivity of Curcumin Using Chitosan-Coated Liposomes through a Combination Mode of High-Pressure Processing","volume":"168","author":"Chen","year":"2022","journal-title":"LWT"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"136815","DOI":"10.1016\/j.ijbiomac.2024.136815","article-title":"Preparation and Characterization of Moringin-Loaded Chitosan-Coated Liposomes and Their Antibacterial Activity against Staphylococcus Aureus","volume":"282","author":"Wen","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"11119","DOI":"10.1021\/jf4035404","article-title":"Preparation of Chitosan-Coated Nanoliposomes for Improving the Mucoadhesive Property of Curcumin Using the Ethanol Injection Method","volume":"61","author":"Shin","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","first-page":"100925","article-title":"Chitosan-Coated Nanoliposomes as Vehicles for l-Citrulline: Preparation, Characterization, Antioxidant Properties, and Nanovesicles Behavior","volume":"11","author":"Mohseni","year":"2025","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"119664","DOI":"10.1016\/j.carbpol.2022.119664","article-title":"Effect of Molecular Weight of Chitosan on the Formation and Properties of Zein-Nisin-Chitosan Nanocomplexes","volume":"292","author":"Cai","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"19161","DOI":"10.1038\/s41598-025-03832-9","article-title":"Preparation and Characteristics Evaluation of Chitosan-Coated Nanoliposomes Containing Ferrous Sulfate","volume":"15","author":"Shirnoush","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_19","unstructured":"Sierra Alcal\u00e1, M. (2019). Obtenci\u00f3n y Caracterizaci\u00f3n de Nanoliposomas Recubiertos Con Pol\u00edmeros de Quitosano Con Diferente Peso Molecular Cargados Con Ampicilina Trihidratada, Universidad ICESI."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"132063","DOI":"10.1016\/j.ijbiomac.2024.132063","article-title":"Layer-by-Layer Coated Probiotics with Chitosan and Liposomes Exhibit Enhanced Therapeutic Effects for DSS-Induced Colitis in Mice","volume":"269","author":"Han","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1039\/D0NR06104B","article-title":"Layer-by-Layer Coated Nanoliposomes for Oral Delivery of Insulin","volume":"13","author":"Zhang","year":"2021","journal-title":"Nanoscale"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"136680","DOI":"10.1016\/j.foodchem.2023.136680","article-title":"Design and Advanced Characterization of Quercetin-Loaded Nano-Liposomes Prepared by High-Pressure Homogenization","volume":"428","author":"Melchior","year":"2023","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Li, X., Zhao, X., Wang, J., Xu, B., Feng, J., and Huang, W. (2025). High-Pressure Microfluidic Homogenization Improves the Stability and Antioxidant Properties of Coenzyme Q10 Nanoliposomes. Biology, 14.","DOI":"10.3390\/biology14050568"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"de Barros, C.T., Alves, T.F.R., Crescencio, K.M.M., Asami, J., Hausen, M.d.A., Duek, E.A.d.R., and Chaud, M.V. (2025). Chitosan-Coated Liposomes for Intranasal Delivery of Ghrelin: Enhancing Bioavailability to the Central Nervous System. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17111493"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yarce, C.J., Alhajj, M.J., Sanchez, J.D., O\u00f1ate-Garz\u00f3n, J., and Salamanca, C.H. (2020). Development of Antioxidant-Loaded Nanoliposomes Employing Lecithins with Different Purity Grades. Molecules, 25.","DOI":"10.3390\/molecules25225344"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"13633","DOI":"10.1021\/acs.langmuir.5b02969","article-title":"Approximate Analytic Expression for the Electrophoretic Mobility of Moderately Charged Cylindrical Colloidal Particles","volume":"31","author":"Ohshima","year":"2015","journal-title":"Langmuir"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0001-8686(98)00087-6","article-title":"Contact Angle Measurement and Contact Angle Interpretation","volume":"81","author":"Kwok","year":"1999","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"109801","DOI":"10.1016\/j.jfoodeng.2019.109801","article-title":"Improvement of the Physical Stability of Oil-in-Water Nanoemulsions Elaborated with Sacha Inchi Oil Employing Ultra-High-Pressure Homogenization","volume":"273","author":"Rave","year":"2020","journal-title":"J. Food Eng."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Michaelides, E.E. (2026). The Equation of Motion of Particles in Fluids\u2014An Historical Perspective. Powders, 5.","DOI":"10.3390\/powders5010005"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"6224","DOI":"10.1021\/la960224o","article-title":"Improved Particle Size Distribution Measurements Using Multiangle Dynamic Light Scattering. 2. Refinements and Applications","volume":"12","author":"Bryant","year":"1996","journal-title":"Langmuir"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Ar\u00e9valo, L.M., Yarce, C.J., O\u00f1ate-Garz\u00f3n, J., and Salamanca, C.H. (2019). Decrease of Antimicrobial Resistance through Polyelectrolyte-Coated Nanoliposomes Loaded with \u03b2-Lactam Drug. Pharmaceuticals, 12.","DOI":"10.3390\/ph12010001"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"113662","DOI":"10.1016\/j.colsurfb.2023.113662","article-title":"Enhancing the Physicochemical Stability and Antioxidant Activity of Cape Gooseberry Calyx Extract through Nanoencapsulation in Soy Lecithin Liposomes","volume":"234","author":"Salamanca","year":"2024","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Pavlovic, N., Mijalkovi\u0107, J., Balan\u010d, B., Lukovi\u0107, N., and Kne\u017eevi\u0107-Jugovi\u0107, Z. (2025). Role of Cholesterol in Modifying the Physical and Stability Properties of Liposomes and In Vitro Release of VitaminB12. Eng. Proc., 99.","DOI":"10.3390\/engproc2025099010"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Khodadadi, E., Khodadadi, E., Chaturvedi, P., and Moradi, M. (2025). Comprehensive Insights into the Cholesterol-Mediated Modulation of Membrane Function Through Molecular Dynamics Simulations. Membranes, 15.","DOI":"10.3390\/membranes15060173"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Laverde-Rojas, V., Liscano, Y., Rivera-S\u00e1nchez, S.P., Ocampo-Ib\u00e1\u00f1ez, I.D., Betancourt, Y., Alhajj, M.J., Yarce, C.J., Salamanca, C.H., and O\u00f1ate-Garz\u00f3n, J. (2021). Antimicrobial Contribution of Chitosan Surface-Modified Nanoliposomes Combined with Colistin against Sensitive and Colistin-Resistant Clinical Pseudomonas Aeruginosa. Pharmaceutics, 13.","DOI":"10.3390\/pharmaceutics13010041"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Perdomo, I., Mora, C., Pinillos, J., O\u00f1ate-Garz\u00f3n, J., and Salamanca, C.H. (2025). Development, Characterization, and Antimicrobial Evaluation of Hybrid Nanoparticles (HNPs) Based on Phospholipids, Cholesterol, Colistin, and Chitosan Against Multidrug-Resistant Gram-Negative Bacteria. Pharmaceutics, 17.","DOI":"10.3390\/pharmaceutics17020182"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Cao, Y., Dong, X., and Chen, X. (2022). Polymer-Modified Liposomes for Drug Delivery: From Fundamentals to Applications. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14040778"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"102730","DOI":"10.1016\/j.jddst.2021.102730","article-title":"Polyelectrolytic Nature of Chitosan: Influence on Physicochemical Properties and Synthesis of Nanoparticles","volume":"65","author":"Wani","year":"2021","journal-title":"J. Drug Deliv. Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Alves, S.J.d.M., Santos, M.A., Neto, J.E.d.S., da Silva, H.N., Barbosa, M.C.S., Fook, M.V.L., Navarro, R.F., and Silva, S.M.d.L. (2025). Combined Effect of PH and Neutralizing Solution Molarity on the Rheological Properties of Chitosan Hydrogels for Biomedical Applications. Gels, 11.","DOI":"10.20944\/preprints202503.1010.v1"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1016\/j.ijbiomac.2018.10.128","article-title":"Dissolution Behavior and Conformation Change of Chitosan in Concentrated Chitosan Hydrochloric Acid Solution and Comparison with Dilute and Semidilute Solutions","volume":"121","author":"Wu","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8244","DOI":"10.1021\/acs.analchem.0c00475","article-title":"Rheology of the Electric Double Layer in Electrolyte Solutions","volume":"92","author":"Funari","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Delas, T., Mock-Joubert, M., Faivre, J., Hofmaier, M., Sandre, O., Dole, F., Chapel, J.P., Cr\u00e9pet, A., Trombotto, S., and Delair, T. (2019). Effects of Chain Length of Chitosan Oligosaccharides on Solution Properties and Complexation with SiRNA. Polymers, 11.","DOI":"10.3390\/polym11081236"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1515\/arh-2019-0010","article-title":"Preparation, Characterization and Rheological Behavior of Glutathione-Chitosan Conjugates in Aqueous Media","volume":"29","author":"Ciro","year":"2019","journal-title":"Appl. Rheol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Aranaz, I., Alc\u00e1ntara, A.R., Civera, M.C., Arias, C., Elorza, B., Heras Caballero, A., and Acosta, N. (2021). Chitosan: An Overview of Its Properties and Applications. Polymers, 13.","DOI":"10.3390\/polym13193256"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"125348","DOI":"10.1016\/j.carbpol.2026.125348","article-title":"Molecular Weight of Chitosan: From Structural Complexity to Analytical Reliability","volume":"385","author":"Zubareva","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"9896","DOI":"10.1021\/la034410n","article-title":"Static Light Scattering Studies on Chitosan Solutions: From Macromolecular Chains to Colloidal Dispersions","volume":"19","author":"Schatz","year":"2003","journal-title":"Langmuir"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1016\/j.apsb.2019.02.010","article-title":"Cholesterol-Tuned Liposomal Membrane Rigidity Directs Tumor Penetration and Anti-Tumor Effect","volume":"9","author":"Wu","year":"2019","journal-title":"Acta Pharm. Sin. B"},{"key":"ref_48","unstructured":"Weeks, B.S. (2025). Latest Advances in Application of Liposomes and Nanoliposomes, IntechOpen."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"29931","DOI":"10.1038\/s41598-024-80970-6","article-title":"Spontaneous Nanosized Liposome Formation from Crude Dried Lecithin upon Addition of Glycerol","volume":"14","author":"Marangoni","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"108801","DOI":"10.1016\/j.lwt.2019.108801","article-title":"Effect of Homogenization Methods on the Physical Stability of Nutrition Grade Nanoliposomes Used for Encapsulating High Oleic Palm Oil","volume":"118","author":"Ricaurte","year":"2020","journal-title":"LWT"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e29516","DOI":"10.1016\/j.heliyon.2024.e29516","article-title":"Preparation of VC Nanoliposomes by High Pressure Homogenization: Process Optimization and Evaluation of Efficacy, Transdermal Absorption, and Stability","volume":"10","author":"Tang","year":"2024","journal-title":"Heliyon"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Dezhakam, E., Kafili-Hajlari, T., and Naseri, A. (2025). Sample Homogenization. Comprehensive Sampling and Sample Preparation, Elsevier.","DOI":"10.1016\/B978-0-443-15978-7.00024-2"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"9099501","DOI":"10.1155\/ijfo\/9099501","article-title":"Optimization of Instant Beverage Powder Containing Propolis Extract Nanoliposomes","volume":"2024","author":"Mirzazadeh","year":"2024","journal-title":"Int. J. Food Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"042002","DOI":"10.1088\/2632-959X\/ae2041","article-title":"Phytochemical Nanoliposomes: Next-Generation Therapeutics in Precision Oncology","volume":"6","author":"Sowmiya","year":"2025","journal-title":"Nano Express"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1038\/s41598-024-82228-7","article-title":"Characterization and in Vitro Anticancer Study of PEGylated Liposome Dually Loaded with Ferulic Acid and Doxorubicin","volume":"15","author":"Miatmoko","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1021\/la034780b","article-title":"Zeta-Potential Measurements as a Tool To Quantify the Effect of Charged Drugs on the Surface Potential of Egg Phosphatidylcholine Liposomes","volume":"20","author":"Matos","year":"2004","journal-title":"Langmuir"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"N\u00e9meth, Z., Cs\u00f3ka, I., Semnani Jazani, R., Sipos, B., Haspel, H., Kozma, G., K\u00f3nya, Z., and Dob\u00f3, D.G. (2022). Quality by Design-Driven Zeta Potential Optimisation Study of Liposomes with Charge Imparting Membrane Additives. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14091798"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Van Bavel, N., Issler, T., Pang, L., Anikovskiy, M., and Prenner, E.J. (2023). A Simple Method for Synthesis of Chitosan Nanoparticles with Ionic Gelation and Homogenization. Molecules, 28.","DOI":"10.3390\/molecules28114328"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.carbpol.2018.07.059","article-title":"Chitosan Nanoparticles via High-Pressure Homogenization-Assisted Miniemulsion Crosslinking for Mixed-Matrix Membrane Adsorbers","volume":"201","author":"Riegger","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Montero, N., Alhajj, M.J., Sierra, M., O\u00f1ate-Garzon, J., Yarce, C.J., and Salamanca, C.H. (2020). Development of Polyelectrolyte Complex Nanoparticles-PECNs Loaded with Ampicillin by Means of Polyelectrolyte Complexation and Ultra-High Pressure Homogenization (UHPH). Polymers, 12.","DOI":"10.3390\/polym12051168"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"27856","DOI":"10.1038\/s41598-024-78550-9","article-title":"Laminar and Turbulent Flow Effects in High-Pressure Homogenization of Liposomes and Perfluorocarbon Nanoemulsions","volume":"14","author":"Lubitz","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Basak, S., and Das, T.K. (2025). Liposome-Based Drug Delivery Systems: From Laboratory Research to Industrial Production\u2014Instruments and Challenges. ChemEngineering, 9.","DOI":"10.3390\/chemengineering9030056"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.ijpharm.2009.01.001","article-title":"Topical Use of Coenzyme Q10-Loaded Liposomes Coated with Trimethyl Chitosan: Tolerance, Precorneal Retention and Anti-Cataract Effect","volume":"372","author":"Zhang","year":"2009","journal-title":"Int. J. Pharm."},{"key":"ref_64","first-page":"966","article-title":"Hypoglycemic Efficacy of Chitosan-Coated Insulin Liposomes after Oral Administration in Mice","volume":"25","author":"Wu","year":"2004","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_65","first-page":"366","article-title":"Preparation, Optimization and Characterization of Chitosan-Coated Liposomes for Solubility Enhancement of Furosemide: A Model BCS IV Drug","volume":"19","author":"Moslehi","year":"2020","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"9735","DOI":"10.1021\/acsomega.3c09948","article-title":"Novel Chitosan-Coated Liposomes Coloaded with Exemestane and Genistein for an Effective Breast Cancer Therapy","volume":"9","author":"Sharma","year":"2024","journal-title":"ACS Omega"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Sebaaly, C., Trifan, A., Sieniawska, E., and Greige-Gerges, H. (2021). Chitosan-Coating Effect on the Characteristics of Liposomes: A Focus on Bioactive Compounds and Essential Oils: A Review. Processes, 9.","DOI":"10.3390\/pr9030445"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"621","DOI":"10.55262\/fabadeczacilik.1764264","article-title":"Liposomes and Chitosan-Coated Liposomes (Chitosomes) for the Delivery of Letrozole: A Comparative Study","volume":"50","year":"2025","journal-title":"Fabad J. Pharm. Sci."},{"key":"ref_69","first-page":"1","article-title":"Depolymerization of Chitosan Using a High Pressure Homogenizer","volume":"15","author":"Cho","year":"2014","journal-title":"Int. J. Mol. Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1021\/bm8014472","article-title":"Mechanisms Involved During the Ultrasonically Induced Depolymerization of Chitosan: Characterization and Control","volume":"10","author":"Lucas","year":"2009","journal-title":"Biomacromolecules"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1016\/j.foodhyd.2010.11.002","article-title":"Effect of Homogenization Conditions on Physicochemical Properties of Chitosan-Based Film-Forming Dispersions and Films","volume":"25","author":"Vargas","year":"2011","journal-title":"Food Hydrocoll."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"103402","DOI":"10.1016\/j.cis.2025.103402","article-title":"On the Formation and Stability Mechanisms of Diverse Lipid-Based Nanostructures for Drug Delivery","volume":"338","author":"Shah","year":"2025","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Culas, S.M., Kaur, L., Popovich, D.G., and Rashidinejad, A. (2026). Physicochemical Properties, Colloidal Stability, and Encapsulation Efficiency of Lecithin-Based and Chitosan-Coated Liposomes Loaded with Cinnamomum Zeylanicum Bioactives. Appl. Sci., 16.","DOI":"10.3390\/app16083754"}],"container-title":["Polymers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4360\/18\/15\/1853\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T08:30:48Z","timestamp":1785313848000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4360\/18\/15\/1853"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":73,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["polym18151853"],"URL":"https:\/\/doi.org\/10.3390\/polym18151853","relation":{},"ISSN":["2073-4360"],"issn-type":[{"value":"2073-4360","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]}}}