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In this study, electrospun Poly(\u03b5-caprolactone) (PCL) scaffolds functionalized with 10% (PCLGLI10) and 20% (PCLGLI20) glycine were fabricated, physicochemically and mechanically characterized, and evaluated in combination with human Wharton\u2019s jelly mesenchymal stromal cells (hWJ-MSCs). Glycine incorporation reduced fiber diameter to the nanoscale range (140\u2013155 nm) and increased scaffold porosity (~71\u201372%) while preserving mechanical properties compatible with skin. FTIR and X-ray diffraction analyses confirmed glycine incorporation and a polymorphic transition from \u03b1- to \u03b3-glycine during electrospinning. Cell viability remained above 95% in all scaffolds; however, PCLGLI10 significantly enhanced cell proliferation, metabolic activity, and the secretion of VEGF and HGF, mediators associated with angiogenesis and tissue repair. Furthermore, in a guinea pig full-thickness wound model, the PCLGLI10+hWJ-MSCs construct promoted a modulated inflammatory response and more organized collagen deposition. These findings support the potential of glycine-functionalized electrospun scaffolds as a promising strategy for chronic cutaneous ulcer regeneration.<\/jats:p>","DOI":"10.3390\/polym18151920","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:16:29Z","timestamp":1785935789000},"page":"1920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glycine-Functionalized Polycaprolactone Electrospun Nanofibers as Bioactive Scaffolds for Skin Repair"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4579-9058","authenticated-orcid":false,"given":"Leonardo","family":"Prieto-Abello","sequence":"first","affiliation":[{"name":"Unidad de Ingenier\u00eda Tisular, Instituto Distrital de Ciencia, Biotecnolog\u00eda e Innovaci\u00f3n en Salud (IDCBIS), Bogot\u00e1 D.C. 111611, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4882-045X","authenticated-orcid":false,"given":"Liliana","family":"Lizarazo-Fonseca","sequence":"additional","affiliation":[{"name":"Unidad de Ingenier\u00eda Tisular, Instituto Distrital de Ciencia, Biotecnolog\u00eda e Innovaci\u00f3n en Salud (IDCBIS), Bogot\u00e1 D.C. 111611, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5018-9927","authenticated-orcid":false,"given":"Gustavo","family":"Salguero","sequence":"additional","affiliation":[{"name":"Unidad de Ingenier\u00eda Tisular, Instituto Distrital de Ciencia, Biotecnolog\u00eda e Innovaci\u00f3n en Salud (IDCBIS), Bogot\u00e1 D.C. 111611, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Silva-Cote","sequence":"additional","affiliation":[{"name":"Unidad de Ingenier\u00eda Tisular, Instituto Distrital de Ciencia, Biotecnolog\u00eda e Innovaci\u00f3n en Salud (IDCBIS), Bogot\u00e1 D.C. 111611, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"ref_1","first-page":"81","article-title":"Skin Wound Healing: An Update on the Current Knowledge and Concepts","volume":"58","author":"Sorg","year":"2017","journal-title":"Eur. 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