{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:08:02Z","timestamp":1792195682216,"version":"4.4.0"},"reference-count":51,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>The development of 3D scaffolds that enable the reliable evaluation of cell migration remains a critical challenge in tissue engineering and wound-healing-related research. In this work, 3D scaffolds based on Chitosan\/Collagen\/Poly(lactic-co-glycolic acid) (PLGA)\/Hydroxyapatite (HAp) nanoparticles are presented as a promising approach to studying the migration of the 3T3 cell line. The electrospinning technique was employed to fabricate fibers with an average diameter of 0.2 \u03bcm for CH\/Coll, 0.69 \u03bcm for CH\/Coll\/PLGA, and 0.14 \u03bcm for CH\/Coll\/HAp. The electrospinning parameters were optimized, and the properties of the scaffolds were further enhanced by incorporating hydroxyapatite (HAp) and PLGA, thereby improving their regenerative potential and tissue-engineering applicability. Preliminary cell proliferation was monitored in the wound area at 4, 12, 24, and 35 h. The highest cell migration was observed in scaffolds with hydroxyapatite nanoparticles. The resulting fiber matrix demonstrated promising effects on fibroblast migration in an in vitro scratch-assay model using NIH 3T3 cells, relevant to skin tissue repair.<\/jats:p>","DOI":"10.3390\/polym18161964","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T13:46:16Z","timestamp":1786455976000},"page":"1964","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Electrospun Chitosan\/Collagen Fibers Incorporating PLGA and Hydroxyapatite Nanoparticles for In-Vitro 3T3 Fibroblast Migration"],"prefix":"10.3390","volume":"18","author":[{"given":"Laila","family":"Procel-Badillo","sequence":"first","affiliation":[{"name":"School of Physical Sciences and Nanotechnology, Yachay Tech University, Urcuqu\u00ed 100119, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4004-4951","authenticated-orcid":false,"given":"Sarah","family":"Brice\u00f1o","sequence":"additional","affiliation":[{"name":"School of Physical Sciences and Nanotechnology, Yachay Tech University, Urcuqu\u00ed 100119, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenin","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"School of Biological Sciences and Biomedicine, Yachay Tech University, Urcuqu\u00ed 100119, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4526-2429","authenticated-orcid":false,"given":"Gema","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"School of Physical Sciences and Nanotechnology, Yachay Tech University, Urcuqu\u00ed 100119, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20417314231185848","DOI":"10.1177\/20417314231185848","article-title":"New insights into balancing wound healing and scarless skin repair","volume":"14","author":"Zhou","year":"2023","journal-title":"J. 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