{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:07:58Z","timestamp":1789992478175,"version":"4.0.1"},"reference-count":134,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T00:00:00Z","timestamp":1789862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2026A1515011681"],"award-info":[{"award-number":["2026A1515011681"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Polymers"],"abstract":"<jats:p>Ultrasound-assisted deep eutectic solvent (DES) extraction has emerged as a promising green and intensified strategy for recovering natural polysaccharides from plant, algal, fungal, and other biological matrices. By coupling acoustic cavitation with tunable solvent microenvironments, this approach can enhance cell-wall disruption, solvent penetration, mass transfer, and polysaccharide solubilization while reducing reliance on harsh acidic, alkaline, or organic solvents. However, extraction efficiency alone is insufficient to define process quality because ultrasound-assisted DES systems may also reshape the molecular weight distribution, monosaccharide composition, uronic acid or sulfate content, substitution pattern, charge density, conformation, surface morphology, and physicochemical behavior. These structural consequences directly influence downstream bioactivities, including antioxidant, hypoglycemic, prebiotic, anti-inflammatory, and anti-ulcerative colitis effects. This review critically summarizes the mechanistic foundations of ultrasound\u2013DES synergy, analyzes how extraction conditions determine polysaccharide structural outcomes, and highlights the importance of linking structure with functionality. Emerging data-driven approaches, including solvent prescreening, COSMO-RS, and machine learning-assisted process optimization, are also discussed as supporting tools for navigating the multidimensional extraction space. However, their current application to the direct prediction of polysaccharide structural outcomes remains limited. Future progress will require standardized datasets, advanced structural characterization, causal structure\u2013activity validation, and scalable process engineering. Overall, ultrasound-assisted DES extraction should be viewed as a structure-sensitive extraction platform whose performance depends on the coordinated control of solvent properties, acoustic conditions, biomass characteristics, and downstream processing.<\/jats:p>","DOI":"10.3390\/polym18182298","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:05:53Z","timestamp":1789988753000},"page":"2298","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides: Mechanistic Foundations, Structural Consequences, and Process Optimization"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8380-0123","authenticated-orcid":false,"given":"Kit-Leong","family":"Cheong","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8202-9434","authenticated-orcid":false,"given":"Wanzi","family":"Yao","sequence":"additional","affiliation":[{"name":"School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farwa","family":"Abdul Hafeez","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3880-925X","authenticated-orcid":false,"given":"Amanullah","family":"Sabir","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9710-0101","authenticated-orcid":false,"given":"Afifa","family":"Aziz","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanhui","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8027-7357","authenticated-orcid":false,"given":"Udayakumar","family":"Veerabagu","sequence":"additional","affiliation":[{"name":"Institute of Forestry and Engineering, Estonian University of Life Sciences, Kreutzwaldi 56, 51006 Tartu, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123525","DOI":"10.1016\/j.carbpol.2025.123525","article-title":"Structure\u2013function relationship of the brown seaweed Undaria pinnatifida laminaran: Protein kinase C-mediated mucus secretion and gut barrier restoration","volume":"358","author":"Cheong","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.ijbiomac.2015.10.016","article-title":"Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum","volume":"82","author":"Zhang","year":"2016","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"103049","DOI":"10.1016\/j.fochx.2025.103049","article-title":"Impact of extraction methods on soybean hull polysaccharides: Structure and functional properties analysis","volume":"31","author":"Zhang","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2843","DOI":"10.1021\/acsfoodscitech.5c00394","article-title":"Effect of Different Extraction Methods on the Structures and Bioactivities of Polysaccharides from Auricularia auricula-judae","volume":"5","author":"Liu","year":"2025","journal-title":"ACS Food Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125189","DOI":"10.1016\/j.carbpol.2026.125189","article-title":"Advances in marine polysaccharides for the prevention and treatment of diabetes","volume":"381","author":"Qian","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"109744","DOI":"10.1016\/j.carres.2025.109744","article-title":"A systematic review on polysaccharides from Ophiopogonis Radix and Liriopes Radix: Advances in the preparation, structural characterization and pharmacological activities","volume":"559","author":"Wang","year":"2026","journal-title":"Carbohydr. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e71009","DOI":"10.1111\/1750-3841.71009","article-title":"Effects of Extraction Methods on the Structural Characteristics and Bioactivities of Houttuynia cordata Polysaccharides","volume":"91","author":"Li","year":"2026","journal-title":"J. Food Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Cheong, K.-L., Sabir, A., Wang, M., Zhong, S., and Tan, K. (2025). Advancements in the Extraction, Characterization, and Bioactive Potential of Laminaran: A Review. Foods, 14.","DOI":"10.3390\/foods14101683"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.1021\/acs.chemrev.5c00597","article-title":"Deep Eutectic Solvents for Pretreatment of Lignocellulose Biomass: Physical Properties, Solubility Mechanisms, and Their Interactions","volume":"126","author":"Mqoni","year":"2026","journal-title":"Chem. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1039\/D5GC05678K","article-title":"Can deep eutectic systems and algae deliver sustainable bioactives and nutrients? A systematic review","volume":"28","author":"Pastor","year":"2026","journal-title":"Green Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"123683","DOI":"10.1016\/j.carbpol.2025.123683","article-title":"Unlocking the potential of deep eutectic solvents for sustainable pectin extraction","volume":"362","author":"Hadidi","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"122199","DOI":"10.1016\/j.carbpol.2024.122199","article-title":"Deep eutectic solvents as sustainable extraction media for extraction of polysaccharides from natural sources: Status, challenges and prospects","volume":"338","author":"Yahaya","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Ijardar, S.P., Singh, V., and Gardas, R.L. (2022). Revisiting the Physicochemical Properties and Applications of Deep Eutectic Solvents. Molecules, 27.","DOI":"10.3390\/molecules27041368"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"101893","DOI":"10.1016\/j.afres.2026.101893","article-title":"Microwave and ultrasound assisted synergistic extraction of aromatics from food and agro-waste using DES and NADES","volume":"6","author":"Roshni","year":"2026","journal-title":"Appl. Food Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"107500","DOI":"10.1016\/j.ultsonch.2025.107500","article-title":"Ultrasound-assisted extraction with deep eutectic solvents for valorization of agro-food waste: A sustainable and eco-friendly approach","volume":"120","author":"Xu","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"\u015eahin, S., Kurtulba\u015f, E., Toprak\u00e7\u0131, \u0130., Anwar, F., Khan, R., Ci\u011fero\u011flu, Z., \u0130nce Yard\u0131mc\u0131, A., Torun, M., Balc\u0131 Torun, F., and Falsafi, S.R. (2026). Deep Eutectic Solvents for Sustainable Extraction of Bioactive Compounds from Biomass: Mechanistic Insights and Scale-Up Challenges. Molecules, 31.","DOI":"10.3390\/molecules31050880"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Pan, X., Xu, L., Meng, J., Chang, M., Cheng, Y., Geng, X., Guo, D., and Liu, R. (2022). Ultrasound-Assisted Deep Eutectic Solvents Extraction of Polysaccharides From Morchella importuna: Optimization, Physicochemical Properties, and Bioactivities. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.912014"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1080\/10826068.2024.2441914","article-title":"Optimization of ultrasonic-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharides from Eucommia ulmoides","volume":"55","author":"Wen","year":"2025","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"125336","DOI":"10.1016\/j.ijbiomac.2023.125336","article-title":"An efficient approach for extraction of polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera by natural deep eutectic solvent","volume":"244","author":"Qu","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"140412","DOI":"10.1016\/j.ijbiomac.2025.140412","article-title":"Prospects and applications of efficient physical field processing technologies for polysaccharide extraction and quality improvement in edible mushrooms: A systematic review","volume":"301","author":"Zhang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"105811","DOI":"10.1016\/j.biombioe.2020.105811","article-title":"Investigation of pretreatment parameters in the delignification of paddy husks with deep eutectic solvents","volume":"142","author":"Okur","year":"2020","journal-title":"Biomass Bioenergy"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"127057","DOI":"10.1016\/j.biortech.2022.127057","article-title":"Approach to an efficient pretreatment method for rice straw by deep eutectic solvent for high saccharification efficiency","volume":"351","author":"Maibam","year":"2022","journal-title":"Bioresour. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1039\/C8GC00900G","article-title":"Multiple hydrogen bond coordination in three-constituent deep eutectic solvents enhances lignin fractionation from biomass","volume":"20","author":"Xia","year":"2018","journal-title":"Green Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20475","DOI":"10.1021\/acs.iecr.4c02346","article-title":"Investigating the Impact of Water on a Menthol-Based Deep Eutectic Solvent: A Combined Experimental and Molecular Dynamics Study","volume":"63","author":"Olawuyi","year":"2024","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"148530","DOI":"10.1016\/j.ijbiomac.2025.148530","article-title":"Betaine-based deep eutectic solvents extraction enhances the emulsification and antioxidant activity of Camellia oleifera leaves polysaccharides","volume":"331","author":"Xue","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"101978","DOI":"10.1016\/j.scp.2025.101978","article-title":"Metabolomic profiling of purple corn pericarp phytochemicals and their efficient recovery in deep eutectic solvents with ultrasound-assisted extraction","volume":"44","author":"Kumar","year":"2025","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"113388","DOI":"10.1016\/j.jpcs.2025.113388","article-title":"Mechanistic insights into cellulose dissolution in deep eutectic solvents from classical MD simulations","volume":"210","author":"Rezaie","year":"2026","journal-title":"J. Phys. Chem. Solids"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"152045","DOI":"10.1016\/j.ijbiomac.2026.152045","article-title":"Novel supramolecular deep eutectic solvents-mediated lignin extraction and reduced cellulase non-productive binding in lignocellulosic enzymatic hydrolysis","volume":"363","author":"Li","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"149154","DOI":"10.1016\/j.foodchem.2026.149154","article-title":"Sustainable preparation of high-performance cellulose nanocrystals from fresh okara via a green synergistic process for improved Pickering emulsion stability","volume":"514","author":"Tang","year":"2026","journal-title":"Food Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109027","DOI":"10.1016\/j.foodhyd.2023.109027","article-title":"Demonstration of feasibility and effectiveness of deep eutectic solvent-water system extraction of RG-I type pectin from wolfberry based on target polysaccharide, solvent and their interactions","volume":"144","author":"Gu","year":"2023","journal-title":"Food Hydrocoll."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"118113","DOI":"10.1016\/j.carbpol.2021.118113","article-title":"Sequential natural deep eutectic solvent pretreatments of apple pomace: A novel way to promote water extraction of pectin and to tailor its main structural domains","volume":"266","author":"Chen","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"129169","DOI":"10.1016\/j.molliq.2025.129169","article-title":"Water-induced modifications in the physicochemical and structural properties of betaine and choline chloride\u2014Ethylene glycol deep eutectic solvents","volume":"444","author":"Monteiro","year":"2026","journal-title":"J. Mol. Liq."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"117097","DOI":"10.1016\/j.microc.2026.117097","article-title":"Ultrasound-assisted extraction of bioactive compounds from Salsola collina pall. using deep eutectic solvents: Optimization, antimicrobial activity, and cytotoxicity assessment","volume":"222","author":"Davydova","year":"2026","journal-title":"Microchem. J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"129563","DOI":"10.1016\/j.molliq.2026.129563","article-title":"Deep eutectic solvents incorporating cannabinoids: The influence of water on stability","volume":"454","author":"Cazzaniga","year":"2026","journal-title":"J. Mol. Liq."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Xue, J., Su, J., Wang, X., Zhang, R., Li, X., Li, Y., Ding, Y., and Chu, X. (2024). Eco-Friendly and Efficient Extraction of Polysaccharides from Acanthopanax senticosus by Ultrasound-Assisted Deep Eutectic Solvent. Molecules, 29.","DOI":"10.3390\/molecules29050942"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Xia, B., Liu, Q., Sun, D., Wang, Y., Wang, W., and Liu, D. (2023). Ultrasound-Assisted Deep Eutectic Solvent Extraction of Polysaccharides from Anji White Tea: Characterization and Comparison with the Conventional Method. Foods, 12.","DOI":"10.3390\/foods12030588"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"100855","DOI":"10.1016\/j.scp.2022.100855","article-title":"Optimization of ultrasonic-assisted deep eutectic solvent for the extraction of polysaccharides from Indocalamus tessellatus leaves and their biological studies","volume":"30","author":"Zhang","year":"2022","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"110405","DOI":"10.1016\/j.cep.2025.110405","article-title":"The new choice for ultrasonic-assisted extraction of Laminaria japonica polysaccharides: A green and efficient deep eutectic solvent","volume":"216","author":"Yan","year":"2025","journal-title":"Chem. Eng. Process.-Process Intensif."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"133308","DOI":"10.1016\/j.ijbiomac.2024.133308","article-title":"Ultrasound-assisted deep eutectic solvents extraction of polysaccharides from Loquat leaf: Process optimization and bioactivity study","volume":"274","author":"Yan","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"116859","DOI":"10.1016\/j.foodres.2025.116859","article-title":"Eco-friendly extraction of polysaccharides from Moringa oleifera Lam. leaves using COSMO-RS screened natural deep eutectic solvents","volume":"217","author":"Peng","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1007\/s12010-024-04990-8","article-title":"Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction, Characterization, and Bioactivities of Polysaccharide from Pericarpium Citri Reticulatae","volume":"196","author":"Wen","year":"2024","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2300272","DOI":"10.1002\/star.202300272","article-title":"Optimization of Ultrasound-Assisted Deep Eutectic Solvent Extraction and Bioactivities of Polysaccharide from Porphyra haitanensis","volume":"77","author":"Hu","year":"2025","journal-title":"Starch"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6513","DOI":"10.1007\/s11694-025-03424-1","article-title":"Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of polysaccharide from the fruit of Rosa roxburghii Tratt","volume":"19","author":"Wen","year":"2025","journal-title":"J. Food Meas. Charact."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"107206","DOI":"10.1016\/j.ultsonch.2024.107206","article-title":"Extraction of American ginseng polysaccharide by ultrasound-assisted deep eutectic solvents-based three-phase partitioning: Process optimization, structural characterization, and anti-ulcerative colitis study","volume":"112","author":"Wu","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"143757","DOI":"10.1016\/j.ijbiomac.2025.143757","article-title":"Prebiotic potential of Ceratonia siliqua polysaccharides: Ultrasound-assisted extraction enhances gut microbial diversity and metabolic activity","volume":"311","author":"Arachchi","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"107383","DOI":"10.1016\/j.ultsonch.2025.107383","article-title":"Deep eutectic solvent extraction of polysaccharides from Gastrodiae elata with ultrasonic and enzymes assistance: Process optimization, structure, and antioxidant activity","volume":"119","author":"Zhou","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"147484","DOI":"10.1016\/j.ijbiomac.2025.147484","article-title":"Ultrasonic-assisted three-phase partitioning with deep eutectic solvents for extraction of Nostoc commune Vauch polysaccharide and its anti-ulcerative colitis activity","volume":"328","author":"Zhang","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"107715","DOI":"10.1016\/j.ultsonch.2025.107715","article-title":"Ultrasound-Assisted Deep Eutectic Solvent-Based Extraction of Polysaccharides from Okra: Optimization by Response Surface Methodology and Artificial Neural Network Modeling","volume":"124","author":"Imran","year":"2026","journal-title":"Ultrason. Sonochem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"107780","DOI":"10.1016\/j.ultsonch.2026.107780","article-title":"Optimization of deep eutectic solvents-based ultrasonic-assisted three-phase partitioning extraction of polysaccharides from Pleurotus tuber-regium and its anti-ulcerative colitis activity","volume":"127","author":"Dong","year":"2026","journal-title":"Ultrason. Sonochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1080\/10826068.2024.2436952","article-title":"Recent advancements in ultrasound-assisted biomolecule extraction from prokaryotic and eukaryotic cells: A review","volume":"56","author":"Sethi","year":"2026","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"107441","DOI":"10.1016\/j.ultsonch.2025.107441","article-title":"Optimization of ultrasonic-assisted extraction for enhancing the cosmetic potential and structural characterization of polysaccharide-rich extract from waste stem of Trollius Chinensis Bunge","volume":"120","author":"Liu","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"107376","DOI":"10.1016\/j.ultsonch.2025.107376","article-title":"Ultrasound-Assisted sequential processing of barley straw using binary acidic and hydrated ternary deep eutectic solvents for nanocellulose production","volume":"118","author":"Pradhan","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"116283","DOI":"10.1016\/j.rser.2025.116283","article-title":"Potential challenges and guidelines for ultrasound and ultrasound-assisted enzymatic treatment of seaweed biomass","volume":"226","author":"Minardi","year":"2026","journal-title":"Renew. Sustain. Energy Rev."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"107445","DOI":"10.1016\/j.ultsonch.2025.107445","article-title":"Molecular dynamics simulation study of ultrasound induced cavitation","volume":"120","author":"Lin","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"8587","DOI":"10.1080\/10408398.2023.2201834","article-title":"Mechanistic and synergistic aspects of ultrasonics and hydrodynamic cavitation for food processing","volume":"64","author":"Tang","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"20277","DOI":"10.1021\/acsomega.4c11498","article-title":"Unraveling the Thermal Effects of High-Intensity Ultrasound: A Practical Guide to Acoustic Power Determination and Heat Management","volume":"10","author":"Ferraz","year":"2025","journal-title":"ACS Omega"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1039\/D4FD00031E","article-title":"Observation of cavitation dynamics in viscous deep eutectic solvents during power ultrasound sonication","volume":"253","author":"Jacobson","year":"2024","journal-title":"Faraday Discuss."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"137519","DOI":"10.1016\/j.ijbiomac.2024.137519","article-title":"Progress in the preparation, structure and bio-functionality of Dictyophora indusiata polysaccharides: A review","volume":"283","author":"He","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"107210","DOI":"10.1016\/j.ultsonch.2024.107210","article-title":"Ultrasound-assisted extraction of polysaccharide from Allium chinense G. Don epidermal waste: Evaluation of extraction mechanism, physicochemical properties, and bioactivities","volume":"112","author":"Li","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"107477","DOI":"10.1016\/j.ultsonch.2025.107477","article-title":"Comprehensive evaluation of extraction process, physicochemical properties and bioactivities of hawk tea polysaccharides: Comparison of ultrasonic and hot water extraction","volume":"120","author":"Wang","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"109717","DOI":"10.1016\/j.bej.2025.109717","article-title":"An investigation of kinetics and mass transfer parameters during ultrasound-assisted extraction of fucoidan from the brown seaweed Ecklonia maxima","volume":"219","author":"Mapholi","year":"2025","journal-title":"Biochem. Eng. J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"107608","DOI":"10.1016\/j.ultsonch.2025.107608","article-title":"Comparison on structural characterization, chain conformation, and neuroprotective activity of polysaccharides extracted from Albizia julibrissin Durazz. bark with hot water and ultrasound","volume":"122","author":"Duan","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"127967","DOI":"10.1016\/j.seppur.2024.127967","article-title":"Ultrasound assisted intensified enzymatic extraction of total phenolic compounds from pomegranate peels","volume":"350","author":"Patil","year":"2024","journal-title":"Sep. Purif. Technol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"114453","DOI":"10.1016\/j.indcrop.2021.114453","article-title":"Structure and functionality of cassava starch in different deep eutectic solvents\/water mixtures: A comparative study","volume":"177","author":"Zeng","year":"2022","journal-title":"Ind. Crops Prod."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"150920","DOI":"10.1016\/j.ijbiomac.2026.150920","article-title":"Microstructure and emulsion stabilization properties of multi-ultrasound assisted amphiphilic starch\u2013lipid complexes","volume":"349","author":"Shahid","year":"2026","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"107535","DOI":"10.1016\/j.ultsonch.2025.107535","article-title":"Ultrasound-assisted deep eutectic solvent extraction of polysaccharides from Cercis chinensis bark: Optimization, kinetics and antioxidant activities","volume":"121","author":"Shu","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Guo, Y., Song, W., Hu, L., Liu, R., Hamid, I.A., and Quan, J. (2025). Kinetics and Thermodynamics of Ultrasound-Assisted Extraction of Taxanes from Taxus chinensis by Natural Deep Eutectic Solvents. Processes, 13.","DOI":"10.2139\/ssrn.5384453"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"19870","DOI":"10.1039\/D6RA01724J","article-title":"Integrated sonication-assisted deep eutectic solvent for extraction of antibacterial compounds from cacao shells","volume":"16","author":"Kusumastuti","year":"2026","journal-title":"RSC Adv."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"35537","DOI":"10.1007\/s11356-021-14485-2","article-title":"Insights into the relationships between physicochemical properties, solvent performance, and applications of deep eutectic solvents","volume":"28","author":"Zhang","year":"2021","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"138185","DOI":"10.1016\/j.cej.2022.138185","article-title":"Parametrical investigation of acoustic cavitation and extraction enhancement in ultrasonic microreactors","volume":"450","author":"Zhao","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"115663","DOI":"10.1016\/j.trac.2019.115663","article-title":"Ultrasound technology for the extraction of biologically active molecules from plant, animal and marine sources","volume":"122","author":"Ojha","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.procbio.2024.09.005","article-title":"Extraction of polyphenols from Apocynum venetum leaves using customized deep eutectic solvents: Process optimization and antioxidant evaluation","volume":"147","author":"Lv","year":"2024","journal-title":"Process Biochem."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1016\/j.ijbiomac.2019.10.042","article-title":"Study on immunostimulatory activity and extraction process optimization of polysaccharides from Caulerpa lentillifera","volume":"143","author":"Zhang","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"10304","DOI":"10.1007\/s11694-026-04309-7","article-title":"Extraction, physicochemical properties and biological activities of polysaccharides from Cistanche tubulosa: Comparison of different extraction methods","volume":"20","author":"Wu","year":"2026","journal-title":"J. Food Meas. Charact."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"143969","DOI":"10.1016\/j.ijbiomac.2025.143969","article-title":"Effect of various extraction methods on the physicochemical properties, antioxidant, and anti-inflammatory activities of mung bean (Vigna radiata L.) skin polysaccharides","volume":"311","author":"Ding","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/j.ijbiomac.2020.02.018","article-title":"Comparison of structural characterization and antioxidant activity of polysaccharides from jujube (Ziziphus jujuba Mill.) fruit","volume":"149","author":"Ji","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.ijbiomac.2020.02.315","article-title":"An insight into anti-inflammatory effects of natural polysaccharides","volume":"153","author":"Hou","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"145366","DOI":"10.1016\/j.ijbiomac.2025.145366","article-title":"Trends in polysaccharide-based hydrogels for skin anti-aging and skin antioxidant","volume":"319","author":"Cheong","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"124352","DOI":"10.1016\/j.molliq.2024.124352","article-title":"Temperature-switchable deep eutectic solvent extraction of polysaccharides from wolfberry fruits: Process optimization, structure characterization, and antioxidant activity","volume":"398","author":"Cao","year":"2024","journal-title":"J. Mol. Liq."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Wang, M., Veeraperumal, S., Zhong, S., and Cheong, K.-L. (2023). Fucoidan-Derived Functional Oligosaccharides: Recent Developments, Preparation, and Potential Applications. Foods, 12.","DOI":"10.3390\/foods12040878"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"104437","DOI":"10.1016\/j.algal.2025.104437","article-title":"Structuring native red seaweed extracts for 3D printing: Rheological synergies with xanthan and locust bean gums","volume":"92","author":"Oliveira","year":"2025","journal-title":"Algal Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"149051","DOI":"10.1016\/j.ijbiomac.2025.149051","article-title":"From waste to wealth: A review of emerging approaches for utilizing dragon fruit (pitaya) waste polysaccharides in value-added bioproducts","volume":"334","author":"Cheong","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1016\/j.ijbiomac.2021.11.004","article-title":"Effect of inulin on pasting, thermal, rheological properties and in vitro digestibility of pea starch gel","volume":"193","author":"Ji","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"110717","DOI":"10.1016\/j.cep.2026.110717","article-title":"Time-dependent extraction of pectins from jabuticaba peel using hydrothermal, microwave, and ultrasound methods: Physicochemical and morphological characterization","volume":"221","author":"Pinheiro","year":"2026","journal-title":"Chem. Eng. Process.-Process Intensif."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s40538-024-00601-0","article-title":"Date pomace polysaccharide: Ultrasonic-assisted deep eutectic solvent extraction, physicochemical properties, biological activities, gut microbiota modulation, and rheological properties","volume":"11","author":"Bamigbade","year":"2024","journal-title":"Chem. Biol. Technol. Agric."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/j.procbio.2020.03.009","article-title":"Synergized subcritical-ultrasound-assisted aqueous two-phase extraction, purification, and characterization of Lentinus edodes polysaccharides","volume":"95","author":"Chikari","year":"2020","journal-title":"Process Biochem."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"112285","DOI":"10.1016\/j.microc.2024.112285","article-title":"Optimization of polysaccharide extraction from pumpkin seeds using ultrasonic-assisted enzymatic method","volume":"208","author":"Hu","year":"2025","journal-title":"Microchem. J."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"102049","DOI":"10.1016\/j.scp.2025.102049","article-title":"Natural deep eutectic solvent-ultrasonic-enzyme combination assisted extraction: Establishment of an integrated method and application on Astragalus polysaccharides","volume":"46","author":"Ye","year":"2025","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"123276","DOI":"10.1016\/j.carbpol.2025.123276","article-title":"Marine algal polysaccharides: Multifunctional bioactive ingredients for cosmetic formulations","volume":"353","author":"Lu","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_90","first-page":"102300","article-title":"Deep eutectic solvent extraction of polysaccharides from Lycium barbarum residue: Optimization, composition analysis, and antioxidant activity","volume":"23","author":"Ma","year":"2025","journal-title":"J. Agric. Food Res."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Gu, H., Liang, L., Wei, Y., Wang, J., Ma, Y., Shi, J., and Li, B. (2025). Green Extraction of Tea Polysaccharides Using Ultrasonic-Assisted Deep Eutectic Solvents and an Analysis of Their Physicochemical and Antioxidant Properties. Foods, 14.","DOI":"10.3390\/foods14152601"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"5391379","DOI":"10.1155\/2020\/5391379","article-title":"A Review of Isolation, Chemical Properties, and Bioactivities of Polysaccharides from Bletilla striata","volume":"2020","author":"Ji","year":"2020","journal-title":"BioMed Res. Int."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.ijbiomac.2019.09.244","article-title":"Structural characterization of a galacturonic acid-rich polysaccharide from Ziziphus Jujuba cv. Muzao","volume":"147","author":"Ji","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_94","doi-asserted-by":"crossref","unstructured":"Li, X., Wang, J., Huang, G., Jia, Z., Xu, M., and Chen, W. (2025). Ultrasonic-Assisted Extraction of Polysaccharides from Schizochytrium limacinum Meal Using Eutectic Solvents: Structural Characterization and Antioxidant Activity. Foods, 14.","DOI":"10.3390\/foods14111901"},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Chen, Y., Du, H., Qu, W., Sun, C., Chen, Q., Du, Y., Zhang, Z., Guo, Y., Wang, C., and Huang, J. (2025). Comparison of Polygonatum sibiricum Polysaccharides from Different Extraction Methods. Foods, 14.","DOI":"10.3390\/foods14132188"},{"key":"ref_96","doi-asserted-by":"crossref","unstructured":"Wang, M., and Cheong, K.-L. (2023). Preparation, Structural Characterisation, and Bioactivities of Fructans: A Review. Molecules, 28.","DOI":"10.3390\/molecules28041613"},{"key":"ref_97","first-page":"3068829","article-title":"Optimization and Preliminary Physicochemical Characterization of Pectin Extraction from Watermelon Rind (Citrullus lanatus) with Citric Acid","volume":"2022","author":"Karina","year":"2022","journal-title":"Int. J. Food Sci."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"107254","DOI":"10.1016\/j.ultsonch.2025.107254","article-title":"Ultrasound-assisted deep eutectic solvent extraction of flavonol glycosides from Ginkgo biloba: Optimization of efficiency and mechanism","volume":"114","author":"Lai","year":"2025","journal-title":"Ultrason Sonochem."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"120895","DOI":"10.1016\/j.indcrop.2025.120895","article-title":"Ultrasonic cavitation assisted deep eutectic solvent extraction of pectin from waste sweet lime peel: Statistical optimization and energetic analysis","volume":"228","author":"Rai","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"111578","DOI":"10.1016\/j.foodhyd.2025.111578","article-title":"Deep eutectic solvents\/water systems in different pH for extracting pectin from mango peel: Analysis of physicochemical and structural properties","volume":"168","author":"Zhang","year":"2025","journal-title":"Food Hydrocoll."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"106045","DOI":"10.1016\/j.ultsonch.2022.106045","article-title":"The effect of sonication-synergistic natural deep eutectic solvents on extraction yield, structural and physicochemical properties of pectins extracted from mango peels","volume":"86","author":"Chen","year":"2022","journal-title":"Ultrason. Sonochem."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"107256","DOI":"10.1016\/j.ultsonch.2025.107256","article-title":"Sustainable pectin extraction from Riang husk using ultrasound-assisted extraction with deep eutectic solvents and its potential in antipollution products","volume":"114","author":"Saelee","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"146432","DOI":"10.1016\/j.foodchem.2025.146432","article-title":"Deep eutectic solvent-assisted aqueous extraction of pectin and essential oil from Citrus reticulata peel: Physicochemical characteristics and emulsifying properties","volume":"495","author":"Chen","year":"2025","journal-title":"Food Chem."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"109779","DOI":"10.1016\/j.carres.2025.109779","article-title":"A comprehensive review on marine algal polysaccharide-mediated siRNA delivery systems for biofuel production","volume":"560","author":"Dinakarkumar","year":"2025","journal-title":"Carbohydr. Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"102120","DOI":"10.1016\/j.biteb.2025.102120","article-title":"Structural and chemical properties of kappa-carrageenan extracted from macroalgae by deep eutectic solvents and sustainable biopolymer films produced thereof","volume":"30","author":"Alfatah","year":"2025","journal-title":"Bioresour. Technol. Rep."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"90","DOI":"10.22146\/ajche.12272","article-title":"Facilitating Ulvan Extraction from Ulva lactuca via Deep Eutectic Solvent and Peracetic Acid Treatment","volume":"24","author":"Wahyu","year":"2024","journal-title":"ASEAN J. Chem. Eng."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"121111","DOI":"10.1016\/j.indcrop.2025.121111","article-title":"Extraction of polysaccharides from quinoa (Chenopodium quinoa Willd) bran using deep eutectic solvent: Isolation, structural characterization, and hypoglycemic activity","volume":"230","author":"Ren","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"129494","DOI":"10.1016\/j.foodchem.2021.129494","article-title":"Effect of ultrasound-assisted alkaline treatment on functional property modifications of faba bean protein","volume":"354","author":"Alavi","year":"2021","journal-title":"Food Chem."},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Wang, Y., Xu, F., Cheng, J., Wu, X., Xu, J., Li, C., Li, W., Xie, N., Wang, Y., and He, L. (2022). Natural Deep Eutectic Solvent-Assisted Extraction, Structural Characterization, and Immunomodulatory Activity of Polysaccharides from Paecilomyces hepiali. Molecules, 27.","DOI":"10.3390\/molecules27228020"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"117686","DOI":"10.1016\/j.lwt.2025.117686","article-title":"Effects of different extraction methods on structure and functional characteristics of soluble dietary fiber from Dendrocalamus brandisii Munro shoots","volume":"223","author":"Tian","year":"2025","journal-title":"LWT"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"14807","DOI":"10.1021\/acs.jmedchem.4c01550","article-title":"Deep Eutectic Solvents Enhancing Drug Solubility and Its Delivery","volume":"67","author":"Sharma","year":"2024","journal-title":"J. Med. Chem."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.ijbiomac.2022.05.182","article-title":"Ultrasound-induced changes in rheological behavior and hydrophobic microdomains of Lignosus rhinocerotis polysaccharide","volume":"213","author":"Cai","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/s41538-026-00750-6","article-title":"Polysaccharides and the colon mucus barrier: A review of biophysical interactions and functional impacts","volume":"10","author":"Cheong","year":"2026","journal-title":"npj Sci. Food"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"100877","DOI":"10.1016\/j.scp.2022.100877","article-title":"Study on extraction and antioxidant activity of polysaccharides from Radix Bupleuri by natural deep eutectic solvents combined with ultrasound-assisted enzymolysis","volume":"30","author":"Wang","year":"2022","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_115","doi-asserted-by":"crossref","unstructured":"Luo, L., Fan, W., Qin, J., Guo, S., Xiao, H., and Tang, Z. (2023). Study on Process Optimization and Antioxidant Activity of Polysaccharide from Bletilla striata Extracted via Deep Eutectic Solvents. Molecules, 28.","DOI":"10.3390\/molecules28145538"},{"key":"ref_116","doi-asserted-by":"crossref","unstructured":"Nie, J., Chen, D., and Lu, Y. (2020). Deep Eutectic Solvents Based Ultrasonic Extraction of Polysaccharides from Edible Brown Seaweed Sargassum horneri. J. Mar. Sci. Eng., 8.","DOI":"10.3390\/jmse8060440"},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"101166","DOI":"10.1016\/j.scp.2023.101166","article-title":"Effect of deep eutectic solvent extraction on Auricularia auricula polysaccharide solubilization and antioxidant potential","volume":"34","author":"Yao","year":"2023","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"128317","DOI":"10.1016\/j.molliq.2025.128317","article-title":"Machine learning for deep eutectic solvents: Advances in property prediction and molecular design","volume":"437","author":"Sharma","year":"2025","journal-title":"J. Mol. Liq."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"144649","DOI":"10.1016\/j.foodchem.2025.144649","article-title":"Intelligent transformation of ultrasound-assisted novel solvent extraction plant active ingredients: Tools for machine learning and deep learning","volume":"486","author":"Meng","year":"2025","journal-title":"Food Chem."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"135079","DOI":"10.1016\/j.foodchem.2022.135079","article-title":"Evaluating the status quo of deep eutectic solvent in food chemistry. Potentials and limitations","volume":"406","author":"Boateng","year":"2023","journal-title":"Food Chem."},{"key":"ref_121","first-page":"201","article-title":"Deep eutectic solvent-based ultrasonic assisted extraction of polysaccharides and antioxidants from Astragalus hamosus L. seedpod","volume":"9","author":"Tabaraki","year":"2025","journal-title":"Trends Phytochem. Res."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.strusafe.2005.03.010","article-title":"Probabilistic finite element analysis using ANSYS","volume":"28","author":"Reh","year":"2006","journal-title":"Struct. Saf."},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Cysewski, P., Jeli\u0144ski, T., and Przyby\u0142ek, M. (2024). Exploration of the Solubility Hyperspace of Selected Active Pharmaceutical Ingredients in Choline- and Betaine-Based Deep Eutectic Solvents: Machine Learning Modeling and Experimental Validation. Molecules, 29.","DOI":"10.3390\/molecules29204894"},{"key":"ref_124","first-page":"e01536","article-title":"Machine learning-driven optimization of Deep Eutectic Solvents: Accelerating physicochemical properties modeling","volume":"45","author":"Saini","year":"2025","journal-title":"Sustain. Mater. Technol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"117373","DOI":"10.1016\/j.microc.2026.117373","article-title":"Ultrasound-mediated deep eutectic solvent extraction of Gymnema sylvestre polysaccharides: Intensified multidimensional optimization and sustainable green metrics assessment","volume":"223","author":"Hao","year":"2026","journal-title":"Microchem. J."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"3958","DOI":"10.1039\/D3GC04533A","article-title":"DESignSolvents: An open platform for the search and prediction of the physicochemical properties of deep eutectic solvents","volume":"26","author":"Odegova","year":"2024","journal-title":"Green Chem."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"109065","DOI":"10.1016\/j.compchemeng.2025.109065","article-title":"ChemBERTa embeddings and ensemble learning for prediction of density and melting point of deep eutectic solvents with hybrid features","volume":"196","author":"Wu","year":"2025","journal-title":"Comput. Chem. Eng."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"136990","DOI":"10.1016\/j.foodchem.2023.136990","article-title":"Natural deep eutectic solvents for the extraction of lentinan from shiitake mushroom: COSMO-RS screening and ANN-GA optimizing conditions","volume":"430","author":"Wang","year":"2024","journal-title":"Food Chem."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1039\/D2GC04574E","article-title":"Machine learning-assisted data-driven optimization and understanding of the multiple stage process for extraction of polysaccharides and secondary metabolites from natural products","volume":"25","author":"Ma","year":"2023","journal-title":"Green Chem."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"147074","DOI":"10.1016\/j.ijbiomac.2025.147074","article-title":"Sustainable food protein extraction from biomass using deep eutectic solvents: A review","volume":"323","author":"Saleem","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"107635","DOI":"10.1016\/j.ultsonch.2025.107635","article-title":"Optimization of ultrasonic extraction and kinetic modeling of jieduquyuziyin prescription polysaccharides via explainable machine learning","volume":"122","author":"Yao","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"107447","DOI":"10.1016\/j.ultsonch.2025.107447","article-title":"Optimization of ultrasound-assisted extraction of polysaccharides from Akebia Fruit using an artificial neural network model: Characteristics and antioxidant activity","volume":"120","author":"Chen","year":"2025","journal-title":"Ultrason. Sonochem."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"107654","DOI":"10.1016\/j.ultsonch.2025.107654","article-title":"Artificial neural network model-based optimization of Polygonum perfoliatum L. 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