{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T21:07:33Z","timestamp":1781730453549,"version":"3.54.5"},"reference-count":52,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T00:00:00Z","timestamp":1649980800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2020-931"],"award-info":[{"award-number":["075-15-2020-931"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Processes"],"abstract":"<jats:p>Around the globe, only 30\u201350% of the amount of oil estimated to be in reservoirs (\u201coriginal oil in place\u201d) can be obtained using primary and secondary oil recovery methods. Enhanced oil recovery methods are required in the oil processing industry, and the use of microbially produced amphiphilic molecules (biosurfactants) is considered a promising efficient and environmentally friendly method. In the present study, biosurfactants produced by the Pseudomonas putida PP021 isolate were extracted and characterized, and their potential to enhance oil recovery was demonstrated. It was found that the cell-free biosurfactant-containing supernatant decreased the air\u2013water interface tension from 74 to 28 mN m\u22121. Using TLC and FTIR methods, the biosurfactants produced by the isolate were classified as mono- and di-rhamnolipid mixtures. In the isolates\u2019 genome, the genes rhlB and rhlC, encoding enzymes involved in the synthesis of mono- and di-rhamnolipids, respectively, were revealed. Both genes were expressed when the strain was cultivated on glycerol nitrate medium. As follows from the sand-packed column and core flooding simulations, biosurfactants produced by P. putida PP021 significantly enhance the degree of recovery, resulting in additional 27% and 21%, respectively.<\/jats:p>","DOI":"10.3390\/pr10040779","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T02:39:31Z","timestamp":1650335971000},"page":"779","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Di- and Mono-Rhamnolipids Produced by the Pseudomonas putida PP021 Isolate Significantly Enhance the Degree of Recovery of Heavy Oil from the Romashkino Oil Field (Tatarstan, Russia)"],"prefix":"10.3390","volume":"10","author":[{"given":"Liliya","family":"Biktasheva","sequence":"first","affiliation":[{"name":"Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Gordeev","sequence":"additional","affiliation":[{"name":"Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Selivanovskaya","sequence":"additional","affiliation":[{"name":"Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5070-786X","authenticated-orcid":false,"given":"Polina","family":"Galitskaya","sequence":"additional","affiliation":[{"name":"Institute of Environmental Sciences, Kazan Federal University, 18 Kremlyovskaya St., 420008 Kazan, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"309159","DOI":"10.1155\/2014\/309159","article-title":"Microbial enhanced heavy oil recovery by the aid of inhabitant spore-forming bacteria: An insight review","volume":"2014","author":"Shibulal","year":"2014","journal-title":"Sci. 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