{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T10:54:41Z","timestamp":1782471281130,"version":"3.54.5"},"reference-count":57,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T00:00:00Z","timestamp":1660521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Research Foundation"},{"name":"Medical Faculty of the Martin-Luther-University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Background: Propofol is a short-acting anesthetic, which is often used for induction and maintenance of general anesthesia, sedation for mechanically ventilated adults and procedural sedation. Several side effects of propofol are known and a substantial number of patients suffer from post-operative delirium after propofol application. In this study, we analyzed the effect of propofol on the function and protein expression profile on a proteome-wide scale. Methods: We cultured human brain microvascular endothelial cells in absence and presence of propofol and analyzed the permeability of the blood-brain barrier (BBB) by fluorescein passage and protein abundance on a proteome-wide scale by mass spectrometry. Results: Propofol interfered with the function of the blood-brain barrier. This was not due to decreased adhesion of propofol-treated human brain microvascular endothelial cells. The proteomic analysis revealed that some key pathways in these cells were disturbed, such as oxygen metabolism, DNA damage recognition and response to stress. Conclusions: Propofol has strong effects on protein expression which could explain several side effects of propofol.<\/jats:p>","DOI":"10.3390\/proteomes10030028","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T23:44:03Z","timestamp":1660607043000},"page":"28","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Disturbance of Key Cellular Subproteomes upon Propofol Treatment Is Associated with Increased Permeability of the Blood-Brain Barrier"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5243-9857","authenticated-orcid":false,"given":"Timo","family":"L\u00e4ngrich","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Martin-Luther-Universit\u00e4t Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaya","family":"Bork","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Martin-Luther-Universit\u00e4t Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00fcdiger","family":"Horstkorte","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Martin-Luther-Universit\u00e4t Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Weber","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Martin-Luther-Universit\u00e4t Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2379-9623","authenticated-orcid":false,"given":"Britt","family":"Hofmann","sequence":"additional","affiliation":[{"name":"Klinik und Poliklinik f\u00fcr Herzchirurgie, Universit\u00e4tsklinikum Halle (Saale), Ernst-Grube-Str. 20, 06120 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matt","family":"Fuszard","sequence":"additional","affiliation":[{"name":"Core Facility\u2014Proteomic Mass Spectrometry, Proteinzentrum Charles Tanford, Kurt-Mothes-Stra\u00dfe 3a, 06120 Halle (Saale), Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1929-1384","authenticated-orcid":false,"given":"Heidi","family":"Olzscha","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologische Chemie, Martin-Luther-Universit\u00e4t Halle-Wittenberg, Hollystr. 1, 06114 Halle (Saale), Germany"},{"name":"Medical School Hamburg MSH, University of Applied Sciences and Medical University, Institute of Molecular Medicine, Am Sandtorkai 76, 20457 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1084\/jem.20171406","article-title":"Alzheimer\u2019s disease: A matter of blood-brain barrier dysfunction?","volume":"214","author":"Montagne","year":"2017","journal-title":"J. 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