{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:51:39Z","timestamp":1782978699890,"version":"3.54.5"},"reference-count":84,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ophthalmic Research Institute of Australia (ORIA)"},{"name":"Australian Government\u2019s National Collaborative Research Infrastructure Scheme (NCRIS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Neural regeneration and neuroprotection represent strategies for future management of neurodegenerative disorders such as Alzheimer\u2019s disease (AD) or glaucoma. However, the complex molecular mechanisms that are involved in neuroprotection are not clearly understood. A promising candidate that maintains neuroprotective signaling networks is neuroserpin (Serpini1), a serine protease inhibitor expressed in neurons which selectively inhibits extracellular tissue-type plasminogen activator (tPA)\/plasmin and plays a neuroprotective role during ischemic brain injury. Abnormal function of this protein has been implicated in several conditions including stroke, glaucoma, AD, and familial encephalopathy with neuroserpin inclusion bodies (FENIB). Here, we explore the potential biochemical roles of Serpini1 by comparing proteome changes between neuroserpin-deficient (NS\u2212\/\u2212) and control mice, in the retina (RE), optic nerve (ON), frontal cortex (FC), visual cortex (VC), and cerebellum (CB). To achieve this, a multiple-plex quantitative proteomics approach using isobaric tandem mass tag (TMT) technology was employed followed by functional enrichment and protein\u2013protein interaction analysis. We detected around 5000 proteins in each tissue and a pool of 6432 quantified proteins across all regions, resulting in a pool of 1235 differentially expressed proteins (DEPs). Principal component analysis and hierarchical clustering highlighted similarities and differences in the retina compared to various brain regions, as well as differentiating NS\u2212\/\u2212 proteome signatures from control samples. The visual cortex revealed the highest number of DEPs, followed by cerebellar regions. Pathway analysis unveiled region-specific changes, including visual perception, focal adhesion, apoptosis, glutamate receptor activation, and supramolecular fiber organization in RE, ON, FC, VC, and CB, respectively. These novel findings provide comprehensive insights into the region-specific networking of Serpini1 in the central nervous system, further characterizing its potential role as a neuroprotective agent. Data are available via ProteomeXchange with identifier PXD046873.<\/jats:p>","DOI":"10.3390\/proteomes12010007","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T05:43:30Z","timestamp":1710395010000},"page":"7","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Quantitative Proteomics Reveal Region-Specific Alterations in Neuroserpin-Deficient Mouse Brain and Retina: Insights into Serpini1 Function"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9145-6155","authenticated-orcid":false,"given":"Shahab","family":"Mirshahvaladi","sequence":"first","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitin","family":"Chitranshi","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ardeshir","family":"Amirkhani","sequence":"additional","affiliation":[{"name":"Australian Proteome Analysis Facility, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashi","family":"Rajput","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devaraj","family":"Basavarajappa","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshana","family":"Vander Wall","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Pascovici","sequence":"additional","affiliation":[{"name":"Australian Proteome Analysis Facility, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Godinez","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Galliciotti","sequence":"additional","affiliation":[{"name":"Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4291-413X","authenticated-orcid":false,"given":"Joao A.","family":"Paulo","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veer","family":"Gupta","sequence":"additional","affiliation":[{"name":"School of Medicine, Deakin University, Geelong, VIC 3125, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart L.","family":"Graham","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0202-7843","authenticated-orcid":false,"given":"Vivek","family":"Gupta","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Mirzaei","sequence":"additional","affiliation":[{"name":"Macquarie Medical School, Macquarie University, Sydney, NSW 2109, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/B978-0-12-802395-2.00021-3","article-title":"Concepts and classification of neurodegenerative diseases","volume":"Volume 145","author":"Kovacs","year":"2018","journal-title":"Handbook of Clinical Neurology"},{"key":"ref_2","unstructured":"Patterson, C. (2018). World Alzheimer Report 2018, Alzheimer\u2019s Disease International (ADI)."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/ajag.12488","article-title":"Population ageing and the labour force: 2000\u20132015 and 2015\u20132030","volume":"36","author":"Temple","year":"2017","journal-title":"Australas. J. Ageing"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1034\/j.1600-0560.2002.56.x","article-title":"The 2030 problem: Caring for aging baby boomers","volume":"37","author":"Knickman","year":"2002","journal-title":"Health Serv. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1146\/annurev-publhealth-040218-043758","article-title":"Causes and Patterns of Dementia: An Update in the Era of Redefining Alzheimer\u2019s Disease","volume":"40","author":"James","year":"2019","journal-title":"Annu. Rev. Public Health"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S18","DOI":"10.1038\/nrn1434","article-title":"Oxidative stress in neurodegeneration: Cause or consequence?","volume":"10","author":"Andersen","year":"2004","journal-title":"Nat. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jns.2012.06.004","article-title":"Mitochondrial disturbances, excitotoxicity, neuroinflammation and kynurenines: Novel therapeutic strategies for neurodegenerative disorders","volume":"322","author":"Toldi","year":"2012","journal-title":"J. Neurol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13024-022-00524-0","article-title":"Solving neurodegeneration: Common mechanisms and strategies for new treatments","volume":"17","author":"Wareham","year":"2022","journal-title":"Mol. Neurodegener."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.2174\/1568026616666161222121229","article-title":"Synopsis on managment strategies for neurodegenerative disorders: Challenges from bench to bedside in successful drug discovery and development","volume":"17","year":"2017","journal-title":"Curr. Top. Med. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"33","DOI":"10.2741\/1778","article-title":"Neuroserpin","volume":"11","author":"Galliciotti","year":"2006","journal-title":"Front. Biosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"33062","DOI":"10.1074\/jbc.272.52.33062","article-title":"Neuroserpin, a brain-associated inhibitor of tissue plasminogen activator is localized primarily in neurons. Implications for the regulation of motor learning and neuronal survival","volume":"272","author":"Hastings","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1160\/TH03-12-0766","article-title":"Neuroserpin: A selective inhibitor of tissue-type plasminogen activator in the central nervous system","volume":"91","author":"Yepes","year":"2004","journal-title":"Thromb. Haemost."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gelderblom, M., Neumann, M., Ludewig, P., Bernreuther, C., Krasemann, S., Arunachalam, P., Gerloff, C., Glatzel, M., and Magnus, T. (2013). Deficiency in serine protease inhibitor neuroserpin exacerbates ischemic brain injury by increased postischemic inflammation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0063118"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"650","DOI":"10.1101\/lm.045864.117","article-title":"The serine protease inhibitor neuroserpin is required for normal synaptic plasticity and regulates learning and social behavior","volume":"24","author":"Reumann","year":"2017","journal-title":"Learn. Mem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1002\/jnr.21592","article-title":"Neuroserpin regulates N-cadherin-mediated cell adhesion independently of its activity as an inhibitor of tissue plasminogen activator","volume":"86","author":"Lee","year":"2008","journal-title":"J. Neurosci. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4746","DOI":"10.1073\/pnas.081071198","article-title":"Genome-wide expression analysis reveals dysregulation of myelination-related genes in chronic schizophrenia","volume":"98","author":"Hakak","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1471-4159.2009.05894.x","article-title":"Plasminogen activator activity is inhibited while neuroserpin is up-regulated in the Alzheimer disease brain","volume":"109","author":"Fabbro","year":"2009","journal-title":"J. Neurochem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"8412","DOI":"10.1038\/s41598-017-08688-2","article-title":"Glaucoma is associated with plasmin proteolytic activation mediated through oxidative inactivation of neuroserpin","volume":"7","author":"Gupta","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3778","DOI":"10.1007\/s12035-022-02817-3","article-title":"Serpin signatures in prion and Alzheimer\u2019s diseases","volume":"59","author":"Zattoni","year":"2022","journal-title":"Mol. Neurobiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1016\/j.ymthe.2023.03.008","article-title":"Neuroserpin gene therapy inhibits retinal ganglion cell apoptosis and promotes functional preservation in glaucoma","volume":"31","author":"Chitranshi","year":"2023","journal-title":"Mol. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nature19949","article-title":"Mass-spectrometric exploration of proteome structure and function","volume":"537","author":"Aebersold","year":"2016","journal-title":"Nature"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2020.03.004","article-title":"After another decade: LC\u2013MS\/MS became routine in clinical diagnostics","volume":"82","author":"Seger","year":"2020","journal-title":"Clin. Biochem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1080\/14789450.2020.1731309","article-title":"Progress and pitfalls of using isobaric mass tags for proteome profiling","volume":"17","author":"Dayon","year":"2020","journal-title":"Expert. Rev. Proteom."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1021\/acs.jproteome.8b00016","article-title":"Proteome-wide evaluation of two common protein quantification methods","volume":"17","author":"Paulo","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1016\/j.drudis.2017.03.001","article-title":"Technological advances and proteomic applications in drug discovery and target deconvolution: Identification of the pleiotropic effects of statins","volume":"22","author":"Banfi","year":"2017","journal-title":"Drug Discov. Today"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1002\/elps.201200481","article-title":"Application of proteomics to cerebrovascular disease","volume":"33","author":"Ning","year":"2012","journal-title":"Electrophoresis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1038\/s41386-020-00840-3","article-title":"Systems-based proteomics to resolve the biology of Alzheimer\u2019s disease beyond amyloid and tau","volume":"46","author":"Rayaprolu","year":"2021","journal-title":"Neuropsychopharmacology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"19.12.1","DOI":"10.1002\/0471143030.cb1912s44","article-title":"Overview: Generation of gene knockout mice","volume":"44","author":"Hall","year":"2009","journal-title":"Curr. Protoc. Cell Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e90017","DOI":"10.7554\/eLife.90017","article-title":"Comparing mouse and human brains","volume":"12","author":"Wong","year":"2023","journal-title":"eLife"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"eabo0924","DOI":"10.1126\/science.abo0924","article-title":"Connectomic comparison of mouse and human cortex","volume":"377","author":"Loomba","year":"2022","journal-title":"Science"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"627896","DOI":"10.3389\/fnana.2021.627896","article-title":"Neuroserpin is strongly expressed in the developing and adult mouse neocortex but its absence does not perturb cortical lamination and synaptic proteome","volume":"15","author":"Kement","year":"2021","journal-title":"Front. Neuroanat."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/S1044-7431(03)00077-0","article-title":"Impaired explorative behavior and neophobia in genetically modified mice lacking or overexpressing the extracellular serine protease inhibitor neuroserpin","volume":"23","author":"Madani","year":"2003","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Wu, J., Cai, Y., Wu, X., Ying, Y., Tai, Y., and He, M. (2021). Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation. Bio Protoc., 11.","DOI":"10.21769\/BioProtoc.3876"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/0042-6989(73)90123-5","article-title":"Dependence of fast components of the electroretinogram of the isolated rat retina on the ionic environment","volume":"13","author":"Winkler","year":"1973","journal-title":"Vis. Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, K.W. (2011). Neuroproteomics, Humana Press.","DOI":"10.1007\/978-1-61779-111-6"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2480","DOI":"10.1021\/acs.jproteome.8b00235","article-title":"Comparison of In-Solution, FASP, and S-Trap Based Digestion Methods for Bottom-Up Proteomic Studies","volume":"17","author":"Ludwig","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/nbt.2839","article-title":"ProteomeXchange provides globally coordinated proteomics data submission and dissemination","volume":"32","author":"Deutsch","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/978-1-4939-6740-7_5","article-title":"TMT one-stop shop: From reliable sample preparation to computational analysis platform","volume":"1549","author":"Mirzaei","year":"2017","journal-title":"Proteome Bioinform."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Akhmedov, M., Martinelli, A., Geiger, R., and Kwee, I. (2020). Omics Playground: A comprehensive self-service platform for visualization, analytics and exploration of Big Omics Data. NAR Genom. Bioinform., 2.","DOI":"10.1093\/nargab\/lqz019"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1093\/bioinformatics\/btz931","article-title":"ShinyGO: A graphical gene-set enrichment tool for animals and plants","volume":"36","author":"Ge","year":"2019","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/acs.jproteome.8b00702","article-title":"Cytoscape StringApp: Network analysis and visualization of proteomics data","volume":"18","author":"Doncheva","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"157","DOI":"10.12688\/f1000research.22280.1","article-title":"Visualize omics data on networks with Omics Visualizer, a Cytoscape App","volume":"9","author":"Legeay","year":"2020","journal-title":"F1000Research"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1007\/s00441-023-03744-y","article-title":"Quantitative proteomics of sperm tail in asthenozoospermic patients: Exploring the molecular pathways affecting sperm motility","volume":"392","author":"Mirshahvaladi","year":"2023","journal-title":"Cell Tissue Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Taylor, S.C., and Posch, A. (2014). The Design of a Quantitative Western Blot Experiment. BioMed Res. Int., 2014.","DOI":"10.1155\/2014\/361590"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/978-1-4939-7057-5_22","article-title":"Quantitative comparisons of large numbers of human plasma samples using TMT10plex labeling","volume":"1619","author":"Liu","year":"2017","journal-title":"Serum\/Plasma Proteom. Methods Protoc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1074\/mcp.RA119.001472","article-title":"Multibatch TMT reveals false positives, batch effects and missing values","volume":"18","author":"Brenes","year":"2019","journal-title":"Mol. Cell. Proteom."},{"key":"ref_48","first-page":"542","article-title":"Evaluation of advanced precursor determination for tandem mass tag (TMT)-based quantitative proteomics across instrument platforms","volume":"18","author":"Myers","year":"2018","journal-title":"J. Proteome Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Dupree, E.J., Jayathirtha, M., Yorkey, H., Mihasan, M., Petre, B.A., and Darie, C.C. (2020). A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field. Proteomes, 8.","DOI":"10.3390\/proteomes8030014"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/978-3-319-41448-5_3","article-title":"Sample Preparation for Mass Spectrometry-Based Proteomics; from Proteomes to Peptides","volume":"919","author":"Rogers","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"eabk0734","DOI":"10.1126\/sciadv.abk0734","article-title":"The Human Proteoform Project: Defining the human proteome","volume":"7","author":"Smith","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1038\/nchembio.2576","article-title":"How many human proteoforms are there?","volume":"14","author":"Aebersold","year":"2018","journal-title":"Nat. Chem. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1146\/annurev-anchem-071015-041550","article-title":"Progress in Top-Down Proteomics and the Analysis of Proteoforms","volume":"9","author":"Toby","year":"2016","journal-title":"Annu. Rev. Anal. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1056\/NEJM199604043341407","article-title":"Knockout Mice","volume":"334","author":"Majzoub","year":"1996","journal-title":"N. Engl. J. Med."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.2353\/ajpath.2010.100466","article-title":"Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death independently of tissue-type plasminogen activator inhibition","volume":"177","author":"Wu","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_56","first-page":"146","article-title":"Tissue plasminogen activator-independent roles of neuroserpin in the central nervous system","volume":"7","author":"Ma","year":"2012","journal-title":"Neural Regen. Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.semcdb.2016.09.007","article-title":"Physiological and pathological functions of neuroserpin: Regulation of cellular responses through multiple mechanisms","volume":"62","author":"Lee","year":"2017","journal-title":"Semin. Cell Dev. Biol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1006\/geno.1996.4514","article-title":"Human neuroserpin (PI12): cDNA cloning and chromosomal localization to 3q26","volume":"40","author":"Schrimpf","year":"1997","journal-title":"Genomics"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1042\/BJ20061170","article-title":"Identification of a novel targeting sequence for regulated secretion in the serine protease inhibitor neuroserpin","volume":"402","author":"Ishigami","year":"2007","journal-title":"Biochem. J."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1146\/annurev-vision-082114-035334","article-title":"Functional circuitry of the retina","volume":"1","author":"Demb","year":"2015","journal-title":"Annu. Rev. Vis. Sci."},{"key":"ref_61","unstructured":"Selhorst, J.B., and Chen, Y. (2009). Seminars in Neurology, Thieme Medical Publishers."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"9402","DOI":"10.1523\/JNEUROSCI.1671-18.2018","article-title":"More than Just a \u201cMotor\u201d: Recent Surprises from the Frontal Cortex","volume":"38","author":"Ebbesen","year":"2018","journal-title":"J. Neurosci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/S0959-4388(03)00102-8","article-title":"Mouse visual cortex","volume":"13","year":"2003","journal-title":"Curr. Opin. Neurobiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3389\/fnsys.2013.00083","article-title":"New roles for the cerebellum in health and disease","volume":"7","author":"Reeber","year":"2013","journal-title":"Front. Syst. Neurosci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/s41586-023-06569-5","article-title":"Spatial atlas of the mouse central nervous system at molecular resolution","volume":"622","author":"Shi","year":"2023","journal-title":"Nature"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/s41586-023-06818-7","article-title":"The molecular cytoarchitecture of the adult mouse brain","volume":"624","author":"Langlieb","year":"2023","journal-title":"Nature"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"5411","DOI":"10.1016\/j.cell.2023.09.028","article-title":"The proteomic landscape of synaptic diversity across brain regions and cell types","volume":"186","author":"Blok","year":"2023","journal-title":"Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s12031-016-0807-7","article-title":"Neuroserpin attenuates H 2 O 2-induced oxidative stress in hippocampal neurons via AKT and BCL-2 signaling pathways","volume":"61","author":"Cheng","year":"2017","journal-title":"J. Mol. Neurosci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1016\/j.neuint.2013.08.010","article-title":"Neuroserpin up-regulation in the Alzheimer\u2019s disease brain is associated with elevated thyroid hormone receptor-\u03b21 and HuD expression","volume":"63","author":"Subhadra","year":"2013","journal-title":"Neurochem. Int."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Ali, N.H., Al-Kuraishy, H.M., Al-Gareeb, A.I., Alnaaim, S.A., Alexiou, A., Papadakis, M., Saad, H.M., and Batiha, G.E.-S. (2023). The probable role of tissue plasminogen activator\/neuroserpin axis in Alzheimer\u2019s disease: A new perspective. Acta Neurol. Belg., 1\u201312.","DOI":"10.1007\/s13760-023-02403-x"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1126\/science.281.5381.1309","article-title":"Mitochondria and apoptosis","volume":"281","author":"Green","year":"1998","journal-title":"Science"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/j.1469-7793.2000.00057.x","article-title":"Mitochondria and calcium: From cell signalling to cell death","volume":"529","author":"Duchen","year":"2000","journal-title":"J. Physiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3950","DOI":"10.1529\/biophysj.105.070037","article-title":"New insights into the mechanism of permeation through large channels","volume":"89","author":"Komarov","year":"2005","journal-title":"Biophys. J."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.brainres.2014.05.021","article-title":"Voltage-dependent anion channels (VDACs) promote mitophagy to protect neuron from death in an early brain injury following a subarachnoid hemorrhage in rats","volume":"1573","author":"Li","year":"2014","journal-title":"Brain Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1126\/science.1083995","article-title":"VDAC2 inhibits BAK activation and mitochondrial apoptosis","volume":"301","author":"Cheng","year":"2003","journal-title":"Science"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"5509","DOI":"10.1002\/pmic.200900472","article-title":"Proteomic DIGE analysis of the mitochondria-enriched fraction from aged rat skeletal muscle","volume":"9","author":"Ohlendieck","year":"2009","journal-title":"Proteomics"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2837","DOI":"10.1016\/j.febslet.2010.04.066","article-title":"Swapping of the N-terminus of VDAC1 with VDAC3 restores full activity of the channel and confers anti-aging features to the cell","volume":"584","author":"Reina","year":"2010","journal-title":"FEBS Lett."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.molmed.2005.02.009","article-title":"Alzheimer\u2019s disease: A\u03b2, tau and synaptic dysfunction","volume":"11","author":"LaFerla","year":"2005","journal-title":"Trends Mol. Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.4103\/1673-5374.386406","article-title":"Amyloid-beta and tau protein beyond Alzheimer\u2019s disease","volume":"19","author":"Abyadeh","year":"2024","journal-title":"Neural Regen. Res."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1212\/01.wnl.0000271077.82508.a0","article-title":"Plasma and CSF serpins in Alzheimer disease and dementia with Lewy bodies","volume":"69","author":"Nielsen","year":"2007","journal-title":"Neurology"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/j.1749-6632.2002.tb04151.x","article-title":"Gene expression related to synaptogenesis, neuritogenesis, and MAP kinase in behavioral sensitization to psychostimulants","volume":"965","author":"Ujike","year":"2002","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4130","DOI":"10.1038\/s41467-018-06642-y","article-title":"Enhanced validation of antibodies for research applications","volume":"9","author":"Edfors","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1800222","DOI":"10.1002\/pmic.201800222","article-title":"The art of validating quantitative proteomics data","volume":"18","author":"Handler","year":"2018","journal-title":"Proteomics"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"610798","DOI":"10.3389\/fgene.2020.610798","article-title":"State of the field in multi-omics research: From computational needs to data mining and sharing","volume":"11","author":"Krassowski","year":"2020","journal-title":"Front. Genet."}],"container-title":["Proteomes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2227-7382\/12\/1\/7\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:13:14Z","timestamp":1760105594000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2227-7382\/12\/1\/7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,14]]},"references-count":84,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,3]]}},"alternative-id":["proteomes12010007"],"URL":"https:\/\/doi.org\/10.3390\/proteomes12010007","relation":{},"ISSN":["2227-7382"],"issn-type":[{"value":"2227-7382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,14]]}}}