{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:13:14Z","timestamp":1790662394320,"version":"4.1.0"},"reference-count":58,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:00:00Z","timestamp":1760054400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022077","name":"INRAE","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"crossref"}]},{"name":"EU FP7 Marie Curie Actions ITN (Initial Training Network) EID (European Industrial Doctorates) DISCo project","award":["FP7-PEOPLE-607611"],"award-info":[{"award-number":["FP7-PEOPLE-607611"]}]},{"name":"R\u00e9gion Auvergne FRI-IRP (Fond R\u00e9gional Innovation\u2014Institut de Recherche Pharmabiotique) CoMBa project"},{"name":"ANR (Agence National de la Recherche Agronomique) PathoFood project","award":["ANR-17-CE21-0002"],"award-info":[{"award-number":["ANR-17-CE21-0002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Background: EHEC O157:H7 causes severe gastrointestinal illness by first colonizing the large intestine. It intimately attaches to the epithelial lining, orchestrating distinctive \u201cattaching and effacing\u201d lesions that disrupt the host\u2019s cellular landscape. While much is known about the well-established virulence factors, there are much to learn about the surface proteins\u2019 roles in a living host. Methods: This study presents the first in vivo characterisation of the surface proteome, i.e., proteosurfaceome, of Escherichia coli O157:H7 EDL933 during intestinal infection, revealing spatial and temporal adaptations critical for colonisation and survival. Using a murine ileal loop model, surface proteomic profiles were analysed at early (3 h) and late (10 h) infection stages across the ileum and colon. Results: In total, 272 proteins were identified, with only 13 shared across all conditions, reflecting substantial niche-specific adaptations. Gene ontology enrichment analyses highlighted dominant roles in metabolic, cellular, and binding functions, while subcellular localisation prediction uncovered cytoplasmic moonlighting proteins with surface activity. Comparative analyses revealed dynamic changes in protein abundance. Conclusions: These findings indicate a coordinated shift from stress adaptation and virulence to nutrient acquisition and persistence and provide a comprehensive view of EHEC O157:H7 surface proteome dynamics during infection, highlighting key adaptive proteins that may serve as targets for future therapeutic and vaccine strategies.<\/jats:p>","DOI":"10.3390\/proteomes13040052","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:50:16Z","timestamp":1760107816000},"page":"52","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Temporal and Spatial Profiling of Escherichia coli O157:H7 Surface Proteome: Insights into Intestinal Colonisation Dynamics In Vivo"],"prefix":"10.3390","volume":"13","author":[{"given":"Ricardo","family":"Monteiro","sequence":"first","affiliation":[{"name":"Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade-i3S, Universidade do Porto, 4150-564 Porto, Portugal"},{"name":"INRAE, Universit\u00e9 Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France"},{"name":"GSK, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Chafsey","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Cordonnier","sequence":"additional","affiliation":[{"name":"M2iSH, UMR 1071 INSERM, University of Clermont Auvergne, 63001 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-9712","authenticated-orcid":false,"given":"Valentin","family":"Ageorges","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Viala","sequence":"additional","affiliation":[{"name":"INRAE, Plateforme d\u2019Exploration du M\u00e9tabolisme\u2014Composante Prot\u00e9omique (PFEMcp), 63122 Saint-Gen\u00e8s-Champanelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0478-2575","authenticated-orcid":false,"given":"Michel","family":"H\u00e9braud","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France"},{"name":"INRAE, Plateforme d\u2019Exploration du M\u00e9tabolisme\u2014Composante Prot\u00e9omique (PFEMcp), 63122 Saint-Gen\u00e8s-Champanelle, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9910-2709","authenticated-orcid":false,"given":"Val\u00e9rie","family":"Livrelli","sequence":"additional","affiliation":[{"name":"M2iSH, UMR 1071 INSERM, University of Clermont Auvergne, 63001 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3400-8949","authenticated-orcid":false,"given":"Alfredo","family":"Pezzicoli","sequence":"additional","affiliation":[{"name":"GSK, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7800-1404","authenticated-orcid":false,"given":"Mariagrazia","family":"Pizza","sequence":"additional","affiliation":[{"name":"Centre for Bacterial Resistance Biology, Department of Life Sciences, Imperial College, London SW7 2AZ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2986-6417","authenticated-orcid":false,"given":"Micka\u00ebl","family":"Desvaux","sequence":"additional","affiliation":[{"name":"INRAE, Universit\u00e9 Clermont Auvergne, UMR0454 MEDIS, 63000 Clermont-Ferrand, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4014\/jmb.0908.08007","article-title":"A brief overview of Escherichia coli O157:H7 and its plasmid O157","volume":"20","author":"Lim","year":"2010","journal-title":"J. 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