{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:45:33Z","timestamp":1792511133491,"version":"4.5.0"},"reference-count":117,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003327","name":"Cooperative Research Centres, Australian Government Department of Industry","doi-asserted-by":"publisher","award":["CRCPEIGHT000074"],"award-info":[{"award-number":["CRCPEIGHT000074"]}],"id":[{"id":"10.13039\/501100003327","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Background: Increasing summer seawater temperatures pose challenges for Atlantic salmon aquaculture, while brain region-specific responses to elevated temperature remain poorly understood. Methods: Atlantic salmon in the warm treatment (WT) underwent thermal ramping from 15 \u00b0C to 19 \u00b0C, with mortality observed at the end of week 4 (15.78%). The WT temperature was subsequently reduced to 18 \u00b0C in week 5, while the control treatment (CT) was adjusted from 15 \u00b0C to 14 \u00b0C, and the WT (18 \u00b0C) and CT (14 \u00b0C) conditions were maintained thereafter. At the end of the trial (week 10), six brain regions, including the cerebellum (CBE), hypothalamus (HYP), medulla oblongata (MED), optic tectum (OPT), pituitary gland (PIT), and telencephalon (TEL), were analysed using data-independent acquisition mass spectrometry-based proteomics. Results: Over 9000 protein groups were identified per brain region, and exploratory differential abundance analysis revealed predominantly region-specific protein abundance changes. Increased SERPINH1 (HSP47) abundance was observed in HYP, PIT, and TEL, suggesting roles in protein-folding and stress regulation. Functional enrichment analyses indicated differential regulation of translation, transcription, energy metabolism, and metabolic pathways across brain regions in the WT group. Conclusions: This study provides a brain region-specific proteomic resource for Atlantic salmon and advances understanding of molecular responses associated with recovery from a temperature reduction (19 \u00b0C to 18 \u00b0C) and subsequent thermal adjustment at 18 \u00b0C.<\/jats:p>","DOI":"10.3390\/proteomes14030037","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T15:05:42Z","timestamp":1784819142000},"page":"37","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proteomic Profiling Reveals Region-Specific Brain Responses During Acclimation to Elevated Water Temperature in Atlantic Salmon (Salmo salar L.)"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0865-4332","authenticated-orcid":false,"given":"Manojkumar","family":"Chandraprakasham","sequence":"first","affiliation":[{"name":"Institute for Marine & Antarctic Studies, University of Tasmania, Hobart, TAS 7001, Australia"},{"name":"Agriculture & Food, Commonwealth Scientific & Industrial Research Organisation, Hobart, TAS 7004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2985-4205","authenticated-orcid":false,"given":"Gianluca","family":"Amoroso","sequence":"additional","affiliation":[{"name":"Institute for Marine & Antarctic Studies, University of Tasmania, Hobart, TAS 7001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5210-1282","authenticated-orcid":false,"given":"Chris G.","family":"Carter","sequence":"additional","affiliation":[{"name":"Institute for Marine & Antarctic Studies, University of Tasmania, Hobart, TAS 7001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-493X","authenticated-orcid":false,"given":"Chloe J.","family":"English","sequence":"additional","affiliation":[{"name":"School of the Environment, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lambertus","family":"Koster","sequence":"additional","affiliation":[{"name":"Agriculture & Food, Bribie Island Research Centre, Commonwealth Scientific & Industrial Research Organisation, Woorim, QLD 4507, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0152-4394","authenticated-orcid":false,"given":"Richard","family":"Wilson","sequence":"additional","affiliation":[{"name":"Central Science Laboratory, Science and Engineering, University of Tasmania, Hobart, TAS 7001, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0312-7317","authenticated-orcid":false,"given":"Richard S.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Agriculture & Food, Commonwealth Scientific & Industrial Research Organisation, Hobart, TAS 7004, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Mendoza-Porras","sequence":"additional","affiliation":[{"name":"Institute for Marine & Antarctic Studies, University of Tasmania, Hobart, TAS 7001, Australia"},{"name":"Agriculture & Food, Bribie Island Research Centre, Commonwealth Scientific & Industrial Research Organisation, Woorim, QLD 4507, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,23]]},"reference":[{"key":"ref_1","unstructured":"FAO (2024). 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