{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:41:58Z","timestamp":1765827718684,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T00:00:00Z","timestamp":1539820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Proteomes"],"abstract":"<jats:p>Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative proteomic approach to find new, potential plasma AAA biomarker candidates. Pre-fractionated plasma samples from twelve patients with AAA and eight matched controls without aneurysm were analyzed by mass spectrometry applying a tandem mass tag (TMT) technique. Eight proteins were differentially regulated in patients compared to controls, including decreased levels of the enzyme bleomycin hydrolase. The down-regulation of this enzyme was confirmed in an extended validation study using an enzyme-linked immunosorbent assay (ELISA). The TMT-based proteomic approach thus identified novel potential plasma biomarkers for AAA.<\/jats:p>","DOI":"10.3390\/proteomes6040043","type":"journal-article","created":{"date-parts":[[2018,10,18]],"date-time":"2018-10-18T10:55:41Z","timestamp":1539860141000},"page":"43","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of Potential Plasma Biomarkers for Abdominal Aortic Aneurysm Using Tandem Mass Tag Quantitative Proteomics"],"prefix":"10.3390","volume":"6","author":[{"given":"Anders E.","family":"Henriksson","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Sundsvall County Hospital, SE-851 86 Sundsvall, Sweden"},{"name":"Department of Surgery, Sundsvall County Hospital, SE-851 86 Sundsvall, Sweden"},{"name":"Department of Natural Sciences, Mid Sweden University, SE-851 70 Sundsvall, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Lindqvist","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sundsvall County Hospital, SE-851 86 Sundsvall, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1704-2333","authenticated-orcid":false,"given":"Carina","family":"Sihlbom","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rgen","family":"Bergstr\u00f6m","sequence":"additional","affiliation":[{"name":"Proteomics Core Facility, University of Gothenburg, SE-405 30 Gothenburg, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Bylund","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, Mid Sweden University, SE-851 70 Sundsvall, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,10,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1016\/S0140-6736(05)66459-8","article-title":"Abdominal aortic aneurysm","volume":"365","author":"Sakalihasan","year":"2005","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.ejvs.2005.03.009","article-title":"A review of biological factors implicated in abdominal aortic aneurysm rupture","volume":"30","author":"Choke","year":"2005","journal-title":"Eur. 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