{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T10:50:50Z","timestamp":1785927050589,"version":"3.56.0"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T00:00:00Z","timestamp":1751500800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reports"],"abstract":"<jats:p>Backgoround: The genus Pseudomonas encompasses metabolically versatile bacteria widely distributed in diverse environments, including clinical settings. Among these, Pseudomonas kurunegalensis is a recently described environmental species with limited clinical characterization. Objective and Methods: In this study, we report the genomic and phenotypic characterization of a P. kurunegalensis isolate, Pam1317368, recovered from a catheterized urine sample of a post-renal transplant patient without symptoms of urinary tract infection. Initial identification by MALDI-TOF MS misclassified the isolate as Pseudomonas monteilii. Whole-genome sequencing and average nucleotide identity (ANI) analysis (\u226595%) confirmed its identity as P. kurunegalensis. The methodology included genomic DNA extraction, Illumina sequencing, genome assembly, ANI calculation, antimicrobial susceptibility testing, resistance gene identification and phylogenetic analysis. Results: Antimicrobial susceptibility testing revealed multidrug resistance, including carbapenem resistance mediated by the metallo-\u03b2-lactamase gene VIM-2. Additional resistance determinants included genes conferring resistance to fluoroquinolones and aminoglycosides. Phylogenetic analysis placed the isolate within the P. kurunegalensis clade, closely related to environmental strains. Conclusions: Although the clinical significance of this finding remains unclear, the presence of clinically relevant resistance genes in an environmental Pseudomonas species isolated from a human sample highlights the value of genomic surveillance and accurate species-level identification in clinical microbiology.<\/jats:p>","DOI":"10.3390\/reports8030104","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T08:22:07Z","timestamp":1751530927000},"page":"104","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of Pseudomonas kurunegalensis by Whole-Genome Sequencing from a Clinical Sample: New Challenges in Identification"],"prefix":"10.3390","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9715-1604","authenticated-orcid":false,"given":"David","family":"Badenas-Alzugaray","sequence":"first","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Valour","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5263-4414","authenticated-orcid":false,"given":"Alexander","family":"Tristancho-Bar\u00f3","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossi","family":"N\u00fa\u00f1ez-Medina","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3888-8952","authenticated-orcid":false,"given":"Ana Mar\u00eda","family":"Milagro-Beamonte","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3709-1690","authenticated-orcid":false,"given":"Carmen","family":"Torres-Manrique","sequence":"additional","affiliation":[{"name":"Area of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Gilaberte-Ang\u00f3s","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1753-5418","authenticated-orcid":false,"given":"Ana Isabel","family":"L\u00f3pez-Calleja","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7294-245X","authenticated-orcid":false,"given":"Antonio","family":"Rezusta-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Research Group on Infections Difficult to Diagnose and Treat, Institute for Health Research Arag\u00f3n, Miguel Servet University Hospital, 50009 Zaragoza, Spain"},{"name":"Clinical Microbiology Service, Miguel Servet University Hospital, 50009 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1111\/j.1574-6976.2011.00269.x","article-title":"Pseudomonas genomes: Diverse and adaptable","volume":"35","author":"Silby","year":"2011","journal-title":"FEMS Microbiol. 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