{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:40:20Z","timestamp":1785404420386,"version":"3.56.0"},"reference-count":15,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FICIC","award":["PI87\/05"],"award-info":[{"award-number":["PI87\/05"]}]},{"award":["PI87\/05"],"award-info":[{"award-number":["PI87\/05"]}],"id":[{"id":"https:\/\/ror.org\/00xmdg797","id-type":"ROR","asserted-by":"publisher"}]},{"name":"FUNDACI\u00d3N 2000","award":["YR-2000-05"],"award-info":[{"award-number":["YR-2000-05"]}]},{"name":"FUNDACI\u00d3N 2000","award":["ID-2000-05"],"award-info":[{"award-number":["ID-2000-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reports"],"abstract":"<jats:p>Uterine leiomyosarcoma (LMS) is a rare and aggressive malignancy with diagnostic challenges, particularly in cases with overlapping histological features with atypical leiomyoma or smooth muscle tumors of uncertain malignant potential. We report a comparative immunohistochemical analysis of LMS, leiomyoma, and adjacent myometrium obtained from a 40-year-old woman with discordant pathological diagnoses. LMS tissue showed increased Ki67 and NTS\/NTSR1 immunoreactivity together with a distinctive cytoplasmic localization of estrogen receptor beta (ER\u03b2), in contrast to the predominantly nuclear localization observed in leiomyoma and normal myometrium. Importantly, focal areas within adjacent morphologically non-neoplastic myometrium exhibited an immunophenotype resembling LMS, including cytoplasmic ER\u03b2 localization and increased Ki67 and NTS\/NTSR1 expression. These observations suggest a potential association between ER\u03b2 subcellular localization, NTS\/NTSR1 signaling, and molecular alterations occurring during uterine smooth muscle tumorigenesis. However, given the single-case nature of this report, these findings should be considered exploratory and require validation in larger studies. The diagnostic message conveyed by these images may assist in the interpretation of diagnostically challenging cases and provide a basis for future investigation.<\/jats:p>","DOI":"10.3390\/reports9030199","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:03:56Z","timestamp":1782349436000},"page":"199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cytoplasmic ER\u03b2 Localization and NTS\/NTSR1 Expression in Uterine Leiomyosarcoma: An Immunohistochemical Insight"],"prefix":"10.3390","volume":"9","author":[{"given":"Yurena","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Microbiolog\u00eda, Biolog\u00eda Celular y Gen\u00e9tica, \u00c1rea de Biolog\u00eda Celular, Facultad de Ciencias, Secci\u00f3n de Biolog\u00eda, Universidad de La Laguna, 38200 La Laguna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Montes","family":"de Oca","sequence":"additional","affiliation":[{"name":"Hospital Quir\u00f3nsalud Tenerife, 38006 Santa Cruz de Tenerife, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idaira","family":"Dorta","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Microbiolog\u00eda, Biolog\u00eda Celular y Gen\u00e9tica, \u00c1rea de Biolog\u00eda Celular, Facultad de Ciencias, Secci\u00f3n de Biolog\u00eda, Universidad de La Laguna, 38200 La Laguna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-3257","authenticated-orcid":false,"given":"Ricardo","family":"Reyes","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Microbiolog\u00eda, Biolog\u00eda Celular y Gen\u00e9tica, \u00c1rea de Biolog\u00eda Celular, Facultad de Ciencias, Secci\u00f3n de Biolog\u00eda, Universidad de La Laguna, 38200 La Laguna, Spain"},{"name":"Instituto de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSP), 38200 La Laguna, Spain"},{"name":"Instituto de Tecnolog\u00edas Biom\u00e9dicas (ITB), 38206 La Laguna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4367-4139","authenticated-orcid":false,"given":"Aixa R.","family":"Bello","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Microbiolog\u00eda, Biolog\u00eda Celular y Gen\u00e9tica, \u00c1rea de Biolog\u00eda Celular, Facultad de Ciencias, Secci\u00f3n de Biolog\u00eda, Universidad de La Laguna, 38200 La Laguna, Spain"},{"name":"Instituto de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSP), 38200 La Laguna, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1053\/j.semdp.2022.01.006","article-title":"Leiomyoma with nuclear atypia: Rare diseases that present a common diagnostic problem","volume":"39","author":"Wei","year":"2022","journal-title":"Semin. 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Pathol."},{"key":"ref_5","first-page":"680","article-title":"Uterine leiomyosarcoma","volume":"4","author":"Gabriel","year":"2018","journal-title":"Oncol. Res. Treat."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1641","DOI":"10.2147\/IJWH.S388278","article-title":"Leiomyoma with bizarre nuclei: A current update","volume":"14","author":"Guo","year":"2022","journal-title":"Int. J. Women\u2019s Health"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.ygyno.2005.05.019","article-title":"The expression of Ki67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study","volume":"99","author":"Akhan","year":"2005","journal-title":"Gynecol. Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s12964-020-00569-y","article-title":"The role of neurotensin and its receptors in non-gastrointestinal cancers: A review","volume":"18","author":"Nikolaou","year":"2020","journal-title":"Cell Commun. Signal."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bertrand, N., Mougel, R., Riley, G., Bruand, M., Gauchotte, G., and Agopiantz, M. (2024). Neurotensin and its involvement in female hormone-sensitive cancers. Int. J. Mol. Sci., 25.","DOI":"10.20944\/preprints202409.2086.v1"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/j.ygyno.2011.05.025","article-title":"Cytoplasmic expression of estrogen receptor beta (ER\u03b2) predicts poor clinical outcome in advanced serous ovarian cancer","volume":"122","author":"Zannoni","year":"2011","journal-title":"Gynecol. 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